SNAREs are concentrated in cholesterol-dependent clusters that define docking and fusion sites for exocytosis

EMBO Journal

Volumn 20, Issue 9, 2001, Pages 2202-2213

  Lang, Thorsten ^a   Bruns, Dieter ^a   Wenzel, Dirk ^a   Riedel, Dietmar ^a   Holroyd, Phillip ^a   Thiele, Christoph ^b   Jahn, Reinhard ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

Author keywords

Cholesterol; Exocytosis; PC12 cells; SNAREs

Indexed keywords

CHOLESTEROL; DOCKING PROTEIN; HYBRID PROTEIN; SNARE PROTEIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN; TRITON X 100;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONCENTRATION RESPONSE; CONTROLLED STUDY; EXOCYTOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN SECRETION;

ANIMALS; BETA-CYCLODEXTRINS; CELL LINE; CELL MEMBRANE; CHOLESTEROL; CRICETINAE; CYCLODEXTRINS; EXOCYTOSIS; MEMBRANE FUSION; MEMBRANE MICRODOMAINS; MEMBRANE PROTEINS; MEMBRANES, ARTIFICIAL; NERVE TISSUE PROTEINS; QA-SNARE PROTEINS; RATS; RECOMBINANT FUSION PROTEINS; SECRETORY VESICLES; SNARE PROTEINS; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; VESICULAR TRANSPORT PROTEINS;

ANIMALIA; RUMEX;

EID: 0035341309     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.9.2202     Document Type: Article

References (40)

1. Expression of synaptosomal-associated protein SNAP-25 in endocrine anterior pituitary cells
2. Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events
3. A cell-free system for regulated exocytosis in PC12 cells
4. A marker of early amacrine cell development in rat retina
5. The molecular machinery for secretion is conserved from yeast to neurons
6. Structure and function of sphingolipid- and cholesterol-rich membrane rafts
7. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
8. Inhibition of transmitter release correlates with the proteolytic activity of tetanus toxin and botulinus toxin A in individual cultured synapses of Hirudo medicinalis
9. Quantal release of serotonin
10. 2+ and secretory-vesicle dynamics
11. A structural change occurs upon binding of syntaxin to SNAP-25
12. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor
13. Methodologies for the culture and experimental use of the PC12 rat pheochromocytoma cell line
14. Identification of SNAREs involved in regulated exocytosis in the pancreatic acinar cell
15. Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy
16. Lipid domain structure of the plasma membrane revealed by patching of membrane components
17. Resolution of regulated secretion into sequential MgATP-dependent and calcium-dependent stages mediated by distinct cytosolic proteins
18. Relationship between NGF-mediated volume increase and 'priming effect' in fast and slow reacting clones of PC12 pheochromocytoma cells. Role of cAMP
19. Membrane fusion and exocytosis
20. Cholesterol is required for surface transport of influenza virus hemagglutinin
21. A conserved membrane-spanning amino acid motif drives homomeric and supports heteromeric assembly of presynaptic SNARE proteins
22. 2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy
23. Structural analysis of the neuronal SNARE protein syntaxin 1A
24. Mechanisms of synaptic vesicle exocytosis
25. Stages of regulated exocytosis
26. Differential effects of α-, β- and γ-cyclodextrins on human erythrocytes
27. Extraction of cholesterol with methyl-β-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles
28. Retention of prominin in microvilli reveals distinct cholesterol-based lipid microdomains within the apical plasma membrane of epthelial cells
29. Mechanisms of intracellular protein transport
30. Interaction of influenza virus haemagglutinin with sphingolipid-cholesterol membrane domains via its transmembrane domain
31. Functional rafts in cell membranes
32. Transport, docking and exocytosis of single secretory granules in live chromaffin cells
33. Acute cholesterol depletion inhibits clathrin-coated pit budding
34. Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles
35. A technique for ultracryotomy of cell suspensions and tissues
36. A study of positive staining of ultrathin frozen sections
37. Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy
38. Membrane flux through the pore formed by a fusogenic viral envelope protein during cell fusion
39. SNAREpins: Minimal machinery for membrane fusion
40. Inhibition of SNARE complex assembly differentially affects kinetic components of exocytosis