Ca1+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy

Neuron

Volumn 18, Issue 6, 1997, Pages 857-863

  Lang, Thorsten ^a   Wacker, Irene ^a   Steyer, Jürgen ^a   Kaether, Christoph ^b   Wunderlich, Ilse ^a   Soldati, Thierry ^a   Gerdes, Hans Herman ^b   Almers, Wolfhard ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b INF 364   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; CHROMOGRANIN B; DOCKING PROTEIN; DOPAMINE BETA MONOOXYGENASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NEUROPEPTIDE Y; PEPTIDE; POTASSIUM ION; CALCIUM; CHROMOGRANIN; DIAGNOSTIC AGENT; PHOTOPROTEIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE TRANSPORT; CELL STRAIN; CONTROLLED STUDY; EXOCYTOSIS; FLUORESCENCE MICROSCOPY; NEURITE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; RAT; SECRETORY GRANULE; ANIMAL; DEGRANULATION; HUMAN; METABOLISM; METHODOLOGY; PHYSIOLOGY; SECRETION; VIDEORECORDING;

ANIMALS; CALCIUM; CELL DEGRANULATION; CHROMOGRANINS; DOPAMINE BETA-HYDROXYLASE; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; NEURITES; NEUROPEPTIDE Y; PC12 CELLS; PEPTIDES; RATS; RECOMBINANT FUSION PROTEINS; VIDEO RECORDING;

EID: 0030612594     PISSN: 08966273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0896-6273(00)80325-6     Document Type: Article

References (24)

1. Banerjee, A., Martin, T.F.J., and DasGupta, B.R. (1993). Nerve growth factor induces sensitivity to botulinum neurotoxin type A in norepinephrine-secreting PC12 cells. Neurosci. Lett. 164, 93-96.
2. Bauerfeind, R., Jelinek, R., and Huttner, W. (1995). Synaptotagmin I- and II-deficient PC12 cells exhibit calcium-independent, depolarization-induced neurotransmitter release from synaptic-like microvesicles. FEBS Lett. 364, 328-334.
3. Betz, W.J., and Bewick, G.S. (1992). Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction. Science 255, 200-203.
4. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W., and Prasher, D.C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
5. Cole, N.B., Smith, C.L., Sciaky, N., Terasaki, M., Edidin, M., and Lippincott-Schwartz, J. (1996). Diffusional mobility of Golgi proteins in membranes of living cells. Science 273, 797-801.
6. Cormack, B.P., Valdivia, R.H., and Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.
7. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A., and Tsien, R.Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455.
8. Gerdes, H.-H., and Kaether, C. (1996). Green fluorescent protein: applications in cell biology. FEBS Lett. 389, 44-47.
9. Gerisch, G., Albrecht, R., Heizer, C., Hodgkinson, S., and Maniak, M. (1995). Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostelium cells monitored using a green fluorescent protein-coronin fusion protein. Current Biol. 5, 1280-1285.
10. Halban, P.A., and Irminger, J.-C. (1994). Sorting and processing of secretory proteins. Biochem. J. 299, 1-18.
11. Hay, J.C., and Martin, T.F.J. (1992). Resolution of regulated secretion into sequential MgATP-dependent and calcium-dependent stages mediated by distinct cytosolic proteins. J. Cell Biol. 119, 139-151.
12. Heumann, R., Kachel, V., and Thoenen, H. (1983). Relationship between NGF-mediated volume increase and "priming effect" in fast and slow reacting clones of PC12 pheochromocytoma cells. Role of cAMP. Exp. Cell Res. 145, 179-190.
13. Kaether, C., and Gerdes, H.-H. (1995). Visualization of protein transport along the secretory pathway using green fluorescent protein. FEBS Lett. 369, 267-271.
14. Kraszewski, K., Mundigl, O., Daniell, L., Verderio, C., Matteoli, M., and de Camilli, P. (1995). Synaptic vesicle dynamics in living cultured hippocampal neurons visualized with CY3-conjugated antibodies directed against the lumenal domain of synaptotagmin. J. Neurosci. 15, 4328-4342.
15. 2+-dependent noradrenaline release from permeabilised PC12 cells is blocked by botulinum neurotoxin A or its light chain. FEBS Lett. 261, 323-326.
16. Milgram, S.L., Chang, E.Y., and Mains, R.E. (1996). Processing and routing of a membrane-anchored form of pro-neuropeptide Y. Mol. Endocrinol. 10, 837-846.
17. Moore, H.-P.H., Walker, M.D., Lee, F., and Kelly, R.B. (1983). Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35, 531-538.
18. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M., and Pozzan, T. (1995). Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Current Biol. 5, 635-642.
19. Ryan, T.A., Reuter, H., Wendland, B., Schweizer, F.E., Tsien, R.W., and Smith, S.J. (1993). The kinetics of synaptic vesicle recycling measured at single presynaptic boutons. Neuron 11, 713-724.
20. Stout, A.L., and Axelrod, D. (1989). Evanescent field excitation of fluorescence by epi-illumination microscopy. Applied Optics 28, 5237-5242.
21. Tooze, S.A., and Huttner, W.B. (1990). Cell-free protein sorting in the regulated and constitutive secretory pathways. Cell 60, 837-847.
22. van der Hoff, M.J.B., Moorman, A.F.M., and Lamers, W.H. (1992). Electroporation in "intracellular" buffer increases cell survival. Nucleic Acids Res. 20, 2902-2908.
23. Wacker, I., Kaether, C., Krömer, A., Migala, A., Almers, W., and Gerdes, H.-H. (1997). Microtubule-dependent transport of secretory vesicles visualized in real time with a GFP-tagged secretory protein. J. Cell Sci., in press.
24. Wu, L.G., and Betz, W.J. (1996). Nerve activity but not intracellular calcium determines the time course of endocytosis at the frog neuromuscular junction. Neuron 17, 769-779.