Sfil endonuclease activity is strongly influenced by the non-specific sequence in the middle of its recognition site

Nucleic Acids Research

Volumn 29, Issue 7, 2001, Pages 1476-1483

  Williams, S A ^a   Halford, S E ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSINE; DNA; DNA BASE; DOUBLE STRANDED DNA; ENDONUCLEASE; GUANINE; RESTRICTION ENDONUCLEASE; SPACER DNA; ENDODEOXYRIBONUCLEASE SFII; OLIGONUCLEOTIDE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; BASE PAIRING; CONFORMATION; CONTROLLED STUDY; DNA CLEAVAGE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME SUBSTRATE; MEASUREMENT; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; PRIORITY JOURNAL; STEADY STATE; BINDING COMPETITION; BINDING SITE; ENZYME SPECIFICITY; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PROTEIN BINDING;

BASE SEQUENCE; BINDING SITES; BINDING, COMPETITIVE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; OLIGONUCLEOTIDES; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

EID: 0035297698     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. DNA recognition by proteins with the helix-turn-helix motif
2. REBASE-restriction enzymes and methylases
3. Effect of non-contacted bases on the affinity of 434 operator for 434 repressor and Cro
4. DNA twisting and the affinity of bacteriophage 434 operator for bacteriophage 434 repressor
5. Crystal structure of CAP-DNA complex: The DNA is bent by 90°
6. DNA sequence determinants of CAP-induced bending and protein binding affinity
7. Type II restriction endonucleases
8. Type I restriction systems: Sophisticated molecular machines
9. Reactions of BglI and other type II restriction endonucleases with discontinuous recognition sequences
10. Crystal structure of restriction endonuclease BglI bound to its interrupted recognition sequence
11. The DNA sequence recognised by BglI
12. DNA bending and kinking-sequence dependence and function
13. A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus
14. Specificity from the synapsis of DNA elements by the SfiI endonuclease
15. The SfiI restriction endonuclease makes a four-strand DNA break at two copies of its recognition sequence
16. DNA excision by the SfiI restriction endonuclease
17. Recombination by resolvase to analyse DNA communications by the SfiI restriction endonuclease
18. DNA looping by the SfiI restriction endonuclease
19. Alternative geometries of DNA looping: An analysis using the SfiI endonuclease
20. The Cfr10I restriction enzyme is functional as a tetramer
21. Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA
22. DNA cleavage by the EcoRV restriction endonuclease: Roles of divalent metal ions in specificity and catalysis
23. 2+ ions
24. Reactions of the EcoRV restriction endonuclease with fluorescent oligodeoxynucleotides: Identical equilibrium constants for binding to specific and non-specific DNA
25. Sequence-dependent DNA structure: Dinucleotide conformation maps
26. Sequence-dependent DNA structure: Tetranucleotide conformation maps
27. DNA recognition and bending
28. The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments
29. Co-crystal structure of TBP recognizing the minor groove of a TATA element
30. The structure of an oligo(dA)·oligo(dT) tract and its biological implications
31. MPD and DNA bending in crystals and in solution
32. The kinetic mechanism of the EcoRI endonuclease
33. One- and three-dimensional pathways for proteins to reach specific DNA sites
34. Sequence-specific recognition of double helical nucleic acids by proteins
35. Stereochemical outcome of the hydrolysis reaction catalyzed by the EcoRV restriction endonuclease
36. A new method for determining the stereochemistry of DNA cleavage reactions: Application to the SfiI and HpaII restriction endonucleases and to the MuA transposase
37. The role of metals in catalysis by the restriction endonuclease BamHI
38. Structure of BamHI endonuclease bound to DNA: Partial folding and unfolding on DNA binding
39. Chemical basis for enzyme catalysis