The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes

EMBO Journal

Volumn 17, Issue 10, 1998, Pages 2926-2937

  Wagner, John D O ^a   Jankowsky, Eckhard ^a   Company, Mahshid ^a   Pyle, Anna Marie ^a   Abelson, John N ^a,b  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b Columbia University ^*  (United States)

Author keywords

DEAD; H box proteins; mRNA splicing; RNA helicase; Splicing factors

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; DOUBLE STRANDED RNA; MESSENGER RNA; MUTANT PROTEIN; PHOSPHATE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; RNA SPLICING; RNA STRUCTURE; SPLICEOSOME;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; DEAD-BOX RNA HELICASES; FUNGAL PROTEINS; GENETIC COMPLEMENTATION TEST; HYDROLYSIS; MUTATION; PHENOTYPE; RECOMBINANT FUSION PROTEINS; RIBONUCLEOPROTEIN, U4-U6 SMALL NUCLEAR; RNA HELICASES; RNA SPLICING; RNA, DOUBLE-STRANDED; RNA, MESSENGER; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE PROTEINS; SPLICEOSOMES;

EID: 0032525149     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.10.2926     Document Type: Article

References (44)

1. Amaratunga.M. and Lohman.T.M. (1993) Esclierichia coli rep helicase unwinds DNA by an active mechanism. Biochemistry, 32, 6815-6820.
2. ArenasJ.E. and AbelsonJ.N. (1997) Prp43: an RNA helicase-Iike factor involved in spliceosome disassembly. Proc. Natl Acad. Sci. USA, 94, 11798-11802.
3. Auble.D.T., Wang.D., Post.K.W. and Hahn,S. (1997) Molecular analysis of the SNF2/SWI2 protein family member MOT1, an ATP-driven enzyme that dissociates TATA-binding protein from DNA. Mol. Cell. Biol, 17, 4842-4851.
4. Brody, E. and AbelsonJ. (1985) The 'spliceosome': yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. Science. 228, 963-967.
5. Brow, D.A. and Guthrie.C. (1988) Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature, 334, 213-218.
6. Brys, A. and Schwer.B. (1996) Requirement for SLU7 in yeast premRNA splicing is dictated by the distance between the branchpoint and the 3' splice site. RNA, 2, 707-717.
7. Chen J.H. and Lin,R.J. (1990) The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors. Nucleic Acids Res., 18, 6447.
8. Chene, S.C. and AbelsonJ. (1987) Spliceosome assembly in yeast. Genes Der., l, 1014-1027.
9. Claude, A., Arenas.J. and HurwitzJ. (1991) The isolation and characterization of an RNA helicase from nuclear extracts of HeLa cells. J. Biol. Client., 266, 10358-10367.
10. Company, M., Arenas J. and AbelsonJ. (1991) Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes. Nature, 349, 487-193.
11. Ghetti, A., Company.M. and AbelsonJ. (1995) Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs. RNA, 1, 132-145.
12. Gorbalenya, A.E., Koonin.E.V, Donchenko.A.P. and Blinov.V.M. (1989) Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res., 17, 4713-4730.
13. Gross, C.H. and Shuman.S. (1996) Vaccinia virus RNA helicase-nucleic acid specificity in duplex unwinding. J. Virol, 70, 2615-2619.
14. Horowitz, D.S. and Abelson, J. (1993) Stages in the second reaction of pre-mRNA splicing: the final step is ATP independent. Genes Der., 7, 320-329.
15. Kadare.G. and Haenni,A.L. (1997) Virus-encoded RNA helicases. J. Virol., 71, 2583-2590.
16. Kim,S.H. and Lin.R.J. (1993) Pre-mRNA splicing within an assembled yeast spliceosome requires an RNA-dependent ATPase and ATP hydrolysis. Proc. Natl Acad. Sci. USA, 90, 888-892.
17. Kim,S.H. and Lin.R.J. (1996) Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol. Cell. Biol, 16, 6810-6819.
18. Kim.S.H., SmithJ., Claude.A. and Lin,R.J. (1992) The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. EMBO J., 11, 2319-2326.
19. Lamm,G.M., Nicol.S.M., Fuller-Pace.F.V. and Lamond.A.I. (1996) p72: a human nuclear DEAD box protein hiahly related to p68. Nucleic Acids Res., 24, 3739-3747.
20. Lee.C.G. and HurwitzJ. (1992) A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3' to 5' direction. J. Biol. Chem., 267, 4398-M07.
21. Lin, R.J., Lustig, AJ. and Abelson J. (1987) Splicing of yeast nuclear pre-mRN m vitro requires a functional 40S spliceosome and several extrinsic factors. Genes Der., l, 7-18.
22. LodmellJ.S. and Dahlberg.A.E. (1997) A conformational switch in Escherichia coli 16S ribosomal RNA during decoding of messenger RNA. Science, 277, 1262-1267.
23. Lohman.T.M. and Bjornson.K.P. (1996) Mechanisms of helicase-catalyzed DNA unwinding. Anna. Rev. Biochem., 65, 169-214.
24. Moore.MJ., Quer.C.C. and Sharp.P.A. (1993) Splicing of precursors to mRNA by the spliceosome. In Gesteland.R.F. and AtkinsJ.F. (eds), The RNA World. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 303-357.
25. Nilsen.T.W. (1994) RNA-RNA interactions in the spliceosome-unraveling the ties that bind. Cell, 78, 1-4.
26. O'Day.C.L., Dalbadie-McFarland.G. and AbelsonJ. (1996) The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA. J. Biol. Chem., 271, 33261-33267.
27. Ohno.M. and Shimura.Y. (1996) A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Genes Dev. 10, 997-1007.
28. Py,B., Higgins,C.F., Krisch.H.M. and Carpousis.AJ. (1996) A DEAD-box RNA helicase in the Escherichia coli RNA degradosome. Nature, 381, 169-72.
29. Rozen.F., Edery.I., Meerovitch.K., Dever.T.E., Merrick.W.C. and Sonenberg.N. (1990) Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F. Mol. Cell. Biol., 10,1134-1144.
30. Schmid.S.R. and Linder.P. (1992) D-E-A-D protein family of putative RNA helicases. Mol. Microbiol, 6. 283-291.
31. Schwer.B. and Guthrie.C. (1991) PRP16 is an RNA-depcndent ATPase that interacts transiently with the spliceosome. Nature, 349, 494-499.
32. Schwer.B. and Guthrie.C. (1992a) A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. EMBO J., 11, 5033-5039.
33. Schwer.B. and Guthrie.C. (1992b) A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome. Mol. Cell. Biol, 12, 3540-3547.
34. Staley.J. and Guthrie.C. (1998) Mechanical gadgets in the spliceosome: clocks, motors, springs and things. Cell, 92, 315-326.
35. Studier,F.W., Rosenberg.A.H., Dunn,J.J. and Dubendorff.J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol, 185, 60-89.
36. Subramanya,H.S., Bird.L.E., BranniganJ.A. and Wigley.D.B. (1996) Crystal structure of a DExx box DNA helicase. Nature, 384, 379-383.
37. Teigelkamp.S., McGarvey.M., Plumpton.M. and Beggs.J.D. (1994) The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA. EMBO J., 13, 888-897.
38. Trotta,C.R., Miao.F, Arn.E.A., Stevens.S.W., Ho.C.K., Rauhut.R. and Abelson.J.N. (1997) The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases. Cell, 89, 849-858.
39. Vijayraghavan.U., Company.M. and AbelsonJ. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev., 3, 1206-1216.
40. WalkerJ.E., Saraste.M., Runswick.MJ. and Gay.N.J. (1982) Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J., 1, 945-951.
41. Wang.Y., WagnerJ.D.O. and Guthrie.C. (1998) Prplo, a DEAH-box splicing factor, unwinds RNA duplexes in vitro. Curr. Biol., 8,441-451.
42. Wassarman.D.A. and SteitzJ.A. (1991) RNA splicing. Alive with DEAD proteins. Nature, 349, 463-464.
43. Xu.D., Nouraini.S., Field.D., Tang.S.J. and FriesenJ.D. (1996) An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature, 381, 709-713.
44. Yean.S.L. and Lin.R.J. (1991) U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction. Mol. Cell. Biol., 11, 5571-5577.