Development of new assays and improved procedures for the purification of recombinant human chymase

European Journal of Biochemistry

Volumn 268, Issue 22, 2001, Pages 5885-5893

  Ferry, Gilles ^a,b   Gillet, Ludovic ^a   Bruneau, Vincent ^a   Banales, Jesus M ^a   Beauverger, Philippe ^a   Cogé, Francis ^a   Galizzi, Jean Pierre ^a   Scalbert, Elizabeth ^c   Okamoto, Takehiro ^b   Urata, Hidenori ^b   Boutin, Jean A ^a  

^a INSTITUT DE RECHERCHES SERVIER   (France)

^b FUKUOKA UNIVERSITY   (Japan)

^c INSTITUT DE RECHERCHES INTERNATIONALES SERVIER   (France)

Author keywords

Chymase; Inhibition; Purification; Recombinant enzyme; Renin angiotensin sytem

Indexed keywords

1,10 PHENANTHROLINE; APROTININ; BIPYRIDINE; CAPTOPRIL; CHYMASE; CHYMOSTATIN; DIPEPTIDYL PEPTIDASE I; ENZYME ANTIBODY; ENZYME INHIBITOR; ENZYME PRECURSOR; IMMUNOGLOBULIN; LISINOPRIL; RECOMBINANT ENZYME; SD 906; SERINE PROTEINASE INHIBITOR; SOYBEAN TRYPSIN INHIBITOR; UNCLASSIFIED DRUG;

ANGIOGENESIS; ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IC 50; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; RELATIVE DENSITY;

ANIMALS; BASE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHYMASES; COS CELLS; CRICETINAE; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; HUMANS; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES;

EID: 0035171924     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02544.x     Document Type: Article

References (49)

1. Angiotensine II receptors and angiotensin II receptor antagonists
2. Identification of a highly specific chymase as the major angiotensin II-forming enzyme in the human heart
3. Cellular localization and regional distribution of an angiotensin II-forming chymase in the heart
4. Activation of two angiotensin-generating systems in the balloon-injured artery
5. The chymase-angiotensin system in human: Biochemistry, molecular biology and potential role in cardiovascular diseases
6. Structure and functions of human angiotensin I converting enzyme (kininase II)
7. Dipeptide processing activates recombinant human pro-chymase
8. Human prochymase activation: A novel role for heparin in zymogen processing
9. Recombinant expression of human mast cell Proteases chymase and tryptase
10. Production of active recombinant human chymase from a construct containing the enterokinase cleavage site of trypsinogen in place of the native propeptide sequence
11. Characterization of recombinant human chymase expressed in Escherichia coli
12. Production of crystallizable human chymase from a Bacillus subtilis system
13. Secretory production of recombinant human chymase as an active form in Pichia pastoris
14. The oligosaccharides of glycoproteins: Bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties
15. Characterization of chymase from human vascular tissues
16. Vasoconstrictor action of angiotensin I-convertase and the synthetic substrate (Pro11, D-Ala12)-angiotensin I
17. Purification and characterization of angiotensin II-generating chymase from hamster cheek pouch
18. Induction of chymase that forms angiotensin II in the monkey atherosclerotic aorta
19. Cloning of the gene and cDNA for human heart chymase
20. Highly efficient inhibition of human chymase by a (2)-macroglobulin
21. The sequence and reactive site of ecotin. A general inhibitor of pancreatic serine proteases from Escherichia coli
22. A liquid chromatography-assisted assay for angiotensin-converting enzyme (peptidyl dipeptidase) in serum
23. Purification and characterization of a kinin- and angiotensin II-forming enzyme in the dog heart
24. Hemorphins derived from hemoglobin have an inhibitory action on angiotensin converting enzyme activity
25. Sensitive method for quantitation of angiotensin-converting enzyme (ACE) activity in tissue
26. Rapid estimation of angiotensin I converting enzyme activity by high-speed liquid chromatography
27. Converting enzyme activity in liver damage
28. Kinetics of the plasma and lung angiotensin I converting enzymes
29. Serum angiotensin converting enzyme activity in sarcoidosis as measured by a simple radiochemical assay
30. A simple radioassay for angiotensin-converting enzyme
31. Elevation of serum angiotensin-converting-enzyme (ACE) level in sarcoidosis
32. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung
33. Inhibition of human chymase by suramin
34. A sensitive fluorimetric assay for serum angiotensin-converting enzyme
35. Substituted 3-(phenylsulfonyl)-1-phenylimidazolidine-2,4-dione derivatives as novel nonpeptide inhibitors of human heart chymase
36. Selection of a histidine-containing inhibitor of gelatinases through deconvolution of combinatorial tetrapeptide libraries
37. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
38. Chymase: Its pathophysiological roles and inhibitors
39. Rapid and specific conversion of precursor interleukin 1 beta (IL-1 beta) to an active IL-1 species by human mast cell chymase
40. Cleavage of type I procollagen by human mast cell chymase initiates collagen fibril formation and generates a unique carboxyl-terminal propeptide
41. Dog mast cell alpha-chymase activates progelatinase B by cleaving the Phe88-Gln89 and Phe91-Glu92 bonds of the catalytic domain
42. Molecular and cellular mechanisms of angiotensin II mediated cardiovascular and renal diseases
43. Increased expression of mast cell chymase in the lungs of patients with congenital heart disease associated with early pulmonary vascular disease
44. Inhibition of chymase reduces vascular proliferation in dog grafted veins
45. Two distinct forms of human mast cell chymases; differences in affinity for heparin and in distribution in skin, heart and other tissues
46. Assay of tyrosine protein kinase activity from HL-60 cytosol by high-performance liquid chromatography
47. Activation of MMP-9 by neutrophil elastase in an in vivo model of acute lung injury
48. A zinc chelator inhibiting gelatinases exerts potent in vitro anti-invasive effects