Secretory production of recombinant human chymase as an active form in Pichia pastoris

Yeast

Volumn 16, Issue 4, 2000, Pages 315-323

  Nakakubo, Hiroshi ^a   Fukuyama, Hajime ^a   Nakajima, Masahide ^a   Imada, Teruaki ^a   Uno, Shusei ^a   Shiota, Naotaka ^b   Takai, Shinji ^b   Miyazaki, Mizuo ^b   Nakamura, Norifumi ^a  

^a Yoshitomi Pharmaceutical Industries Ltd   (Japan)

^b OSAKA MEDICAL COLLEGE   (Japan)

Author keywords

Chymase; Expression; Glycosylation; Pichia pastoris; Secretion

Indexed keywords

ANGIOTENSIN; ANGIOTENSIN I; ANILIDE; CHYMASE; COMPLEMENTARY DNA; RECOMBINANT ENZYME;

ARTICLE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME RELEASE; HUMAN; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

PICHIA PASTORIS;

EID: 0034653183     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0061(20000315)16:4<315::AID-YEA527>3.0.CO;2-4     Document Type: Article

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