Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore

Nature Biotechnology

Volumn 18, Issue 10 SUPPL., 2000, Pages 1091-1095

  Movileanu, Liviu ^a   Howorka, Stefan ^a   Braha, Orit ^a   Bay ley, Hagan ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Biosensor; Nanostructure; Polymer; Pore; Protein engineering

Indexed keywords

ALPHA HEMOLYSIN; BIOTIN; MACROGOL; MEMBRANE PROTEIN; POLYMER; STAPHYLOCOCCUS ALPHA TOXIN; STREPTAVIDIN; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BIOSENSOR; CELL MEMBRANE TRANSPORT; ION CURRENT; KINETICS; LIPID BILAYER; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ASSAY; SURFACE PLASMON RESONANCE; TIME;

ANTIBODIES, MONOCLONAL; BACTERIAL TOXINS; BIOSENSING TECHNIQUES; BIOTIN; ELECTRIC CONDUCTIVITY; HEMOLYSIN PROTEINS; ION CHANNELS; KINETICS; LIGANDS; LIPID BILAYERS; MODELS, MOLECULAR; MUTATION; PARTICLE SIZE; POLYETHYLENE GLYCOLS; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEINS; SIGNAL TRANSDUCTION; SOLUTIONS; STREPTAVIDIN;

EID: 0033776849     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/80295     Document Type: Article

References (14)

1. Heath, J.R., Kuekes, P.J., Snider, G.S. & Williams, R.S. A defect-tolerant computer architecture: opportunities for nanotechnology. Science 280, 1716-1721 (1998).
2. Davis, A.P. Synthetic molecular motors. Nature 401, 120-121 (1999).
3. Seeman, N.C. DNA engineering and its applications to nanotechnology. Trends Biotechnol. 17, 437-443 (1999).
4. Song, L. et al. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science 274, 1859-1865 (1996).
5. Gouaux, E. α-Hemolysin from Staphylococcus aureus: an archetype of β-barrel, channel-forming toxins. J. Struct. Biol. 121, 110-122 (1998).
6. Chang, C.-Y., Niblack, B., Walker, B. & Bayley, H. A photogenerated pore-forming protein. Chem. Biol. 2, 391-400 (1995).
7. Panchal, R.G., Cusack, E., Cheley, S. & Bayley, H. Tumor protease-activated, pore-forming toxins from a combinatorial library. Nat. Biotechnol. 14, 852-856 (1996).
8. Russo, M.J., Bayley, H. & Toner, M. Reversible permeabilization of plasma membranes with an engineered switchable pore. Nat. Biotechnol. 15, 278-282 (1997).
9. Braha, O. et al. Designed protein pores as components for biosensors. Chem. Biol. 4, 497-505 (1997).
10. Gu, L.-Q., Braha, O., Conlan, S., Cheley, S. & Bayley, H. Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter. Nature 398, 686-690 (1999).
11. Bezrukov, S.M., Vodyanoy, I. & Parsegian, V.A. Counting polymers moving through a single ion channel. Nature 370, 279-281 (1994).
12. Bezrukov, S.M., Vodyanoy, I., Brutyan, R.A. & Kasianowicz, J.J. Dynamics and free energy of polymer partitioning into a nanoscale pore. Macromolecules 29, 8517-8522 (1996).
13. Merzlyak, P.G. et al. Polymeric nonelectrolytes to probe pore geometry: application to the α-toxin transmembrane channel. Biophys. J. 77, 3023-3033 (1999).
14. Kasianowicz, J.J., Brandin, E., Branton, D. & Deamer, D.W. Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. USA 93, 13770-13773 (1996).