A functional protein pore with a 'retro' transmembrane domain

Protein Science

Volumn 8, Issue 6, 1999, Pages 1257-1267

  Cheley, Stephen ^a   Braha, Orit ^a   Lu, Xiaofeng ^a   Conlan, Sean ^a   Bayley, Hagan ^a,b  

^a TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Channel; Membrane protein; Pore; Retro sequence; Reversed sequence; hemolysin; barrel

Indexed keywords

HEMOLYSIN; MEMBRANE PROTEIN;

ARTICLE; CHANNEL GATING; MEMBRANE CHANNEL; MEMBRANE PERMEABILITY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; PRIORITY JOURNAL;

EID: 0032993061     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.6.1257     Document Type: Article

References (46)

1. Bezrukov SM, Vodyanoy I, Brutyan RA, Kasianowicz JJ. 1996. Dynamics and free energy of polymer partitioning into a nanoscale pore. Macromolecules 29:8517-8522.
2. Bhakdi S, Füssle R, Tranum-Jensen J. 1981. Staphylococcal α-toxin: Oligomerization of hydrophilic monomers to form amphiphilic hexamers induced through contact with deoxycholate micelles. Proc Natl Acad Sci USA 78:5475-5479.
3. Bhakdi S, Muhly M, Füssle R. 1984. Correlation between toxin binding and hemolytic activity in membrane damage by staphylococcal α-toxin. Infect Immun 46:318-323.
4. Braha O, Walker B, Cheley S, Kasianowicz JJ, Song L, Gouaux JE, Bayley H. 1997. Designed protein pores as components for biosensors. Chem Biol 4:497-505.
5. Chang C-Y, Niblack B, Walker B, Bayley H. 1995. A photogenerated pore-forming protein. Chem Biol 2:391-400.
6. Cheley S, Malghani MS, Song L, Hobaugh M, Gouaux JE, Yang J, Bayley H. 1997. Spontaneous oligomerization of a staphylococcal α-hemolysin conformationally constrained by removal of residues that form the transmembrane β-barrel. Protein Eng 10:1433-1443.
7. Chorev M, Goodman M. 1993. A dozen years of retro-inverso peptidomimetics. Acc Chem Res 26:266-273.
8. Chorev M, Goodman M. 1995. Recent developments in retro peptides and proteins - An ongoing topochemical exploration. Trends Biotechnol 13:438-445.
9. Christopher JA. 1998. SPOCK: The structural properties observation and calculation kit (program manual). College Station, Texas: Center for Macromolecular Design, Texas A&M University.
10. Derossi D, Calvet S, Trembleau A, Brunissen A, Chassaing G, Prochiantz A. 1996. Cell internalization of the third helix of the Antennapedia homeo-domain is receptor-independent. J Biol Chem 271:18188-18193.
11. Füssle R, Bhakdi S, Sziegoleit A, Tranum-Jensen J, Kranz T, Wellensiek H-J. 1981. On the mechanism of membrane damage by Staphylococcus aureus α-toxin. J Cell Biol 91:83-94.
12. Gouaux E. 1998. α-Hemolysin from S. aureus: An archetype of α-barrel, channel-forming toxins. J Struct Biol 121:110-122.
13. Gouaux JE, Braha O, Hobaugh MR, Song L, Cheley S, Shustak C, Bayley H. 1994. Subunit stoichiometry of staphylococcal α-hemolysin in crystals and on membranes: A heptameric transmembrane pore. Proc Natl Acad Sci USA 91:12828-12831.
14. Guptasarma P. 1992. Reversal of peptide backbone direction may result in the mirroring of protein structure. FEBS Lett 310:205-210.
15. Heinz DW, Baase WA, Matthews BW. 1992. Folding and function of a T4 lysozyme containing 10 consecutive alanines illustrate the redundancy of information in an amino acid sequence. Proc Natl Acad Sci USA 89:3751-3755.
16. Howorka S, Bayley H. 1998. Improved protocol for high-throughput cysteine scanning mutagenesis. Biotechniques 25:766-772.
17. Hutchinson EG, Sessions RB, Thornton JM, Woolfson DN. 1998. Determinants of strand register in antiparallel β-sheets of proteins. Protein Sci 7:2287-2300.
18. Ido Y, Vindigni A, Chang K, Stramm L, Chance R, Heath WF, DiMarchi RD, Di Cera E, Williamson JR. 1997. Prevention of vascular and neural dysfunction in diabetic rats by C-peptide. Science 277:563-567.
19. Jones DH, Howard BH. 1991. A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction. Biotechniques 10:62-66.
20. Kasianowicz JJ, Brandin E, Branton D, Deamer DW. 1996. Characterization of individual polynucleotide molecules using a membrane channel. Proc Natl Acad Sci USA 93:13770-13773.
21. Korchev YE, Bashford CL, Alder GM, Kasianowicz JJ, Pasternak CA. 1995. Low conductance states of a single ion channel are not "closed." J Membrane Biol 147:233-239.
22. Krasilnikov OV, Sabirov RZ, Ternovsky VI, Merzliak PG, Muratkhodjaev JN. 1992. A simple method for the determination of the pore radius of ions channels in planar lipid bilayer membranes. FEMS Microbiol Immunol 105:93-100.
23. Krasilnikov OV, Sabirov RZ, Ternovsky VI, Merzliak PG, Tashmukhamedov BA. 1988. The structure of S. aureus α-toxin induced ionic channel. Gen Physiol Biophys 7:467-473.
24. Lacroix E, Viguera AR, Serrano L. 1998. Reading protein sequences backwards. Folding Design 3:79-85.
25. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
26. Mackenzie KR, Engelman DM. 1998. Structure-based prediction of the stability of transmembrane helix-helix interactions - The sequence dependence of glycophorin A dimerization. Proc Natl Acad Sci USA 95:3583-3590.
27. Matthews BW, Weaver LH, Kester WR. 1974. The conformation of thermolysin. J Biol Chem 249:8030-8044.
28. McDonnell JM, Fushman D, Cahill SM, Sutton BJ, Cowburn D. 1997. Solution structures of Fc∈Rl α-chain mimics: A β-hairpin peptide and its retroenantiomer. J Am Chem Soc 119:5321-5328.
29. Menestrina G. 1986. Ionic channels formed by S. aureus alpha-toxin: Voltage-dependent inhibition by divalent and trivalent cations. J Membrane Biol 90:177-190.
30. Merrifield RB, Juvvadi P, Andreu D, Ubach J, Boman A, Boman HG. 1995. Retro and retroenantio analogs of cecropin-melittin hybrids. Proc Natl Acad Sci USA 92:3449-3453.
31. Middaugh CR, Thomson JA, Burke CJ, Mach H, Naylor AM, Bogusky MJ, Ryan JA, Pitzenberger SM, Ji H, Cordingley JS. 1993. Structure of synthetic peptide analogues of an eggshell protein of Schistosoma mansoni. Protein Sci 2:900-914.
32. Milton RCdeL, Milton SCF, Kent SBH. 1992. Total synthesis of a D-enzyme: The enantiomers of HIV-1 protease show demonstration of reciprocal chiral substrate specificity. Science 256:1445-1448.
33. Montal M, Mueller P. 1972. Formation of bimolecular membranes from lipid monolayers and study of their electrical properties. Proc Natl Acad Sci USA 69:3561-3566.
34. Nicholson H, Söderlind E, Tronrud DE, Matthews BW. 1989. Contributions of left-handed helical residues to the structure and stability of bacteriophage T4 lysozyme. J Mol Biol 210:181-193.
35. Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E. 1999. Crystal structure of staphyococcal lukF delineates conformational changes accompanying formation of a transmembrane channel. Nature Struct Biol 6:134-140.
36. Olszewski KA, Kolinski A, Skolnick J. 1996. Does a backwardly read protein sequence have a unique native state? Protein Eng 9:5-14.
37. Panchal RG, Cusack E, Cheley S, Bayley H. 1996. Tumor protease-activated, pore-forming toxins from a combinatorial library. Nature Biotechnol 14:852-856.
38. Regan L. 1993. What determines where α-helices begin and end? Proc Natl Acad Sci USA 90:10907-10908.
39. Schumacher TNM, Mayr LM, Minor DL, Milhollen MA, Burgess MW, Kim PS. 1996. Identification of D-peptide ligands through mirror-image phuge display. Science 271:1854-1857.
40. Song L, Hobaugh MR, Shustak C, Cheley S, Bayley H, Gouaux JE. 1996. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science 274:1859-1865.
41. Valeva A, Weisser A, Walker B, Kehoe M, Bayley H, Bhakdi S, Palmer M. 1996. Molecular architecture of a toxin pore: A 15-residue sequence lines the trans-membrane channel of staphylococcal alpha-toxin. EMBO J 15:1857-1864.
42. Wade D, Boman A, Wåhlin B, Drain CM, Andreu D, Boman HG, Merrifield RB. 1990. All-D amino acid-containing channel-forming antibiotic peptides. Proc Natl Acad Sci USA 87:4761-4765.
43. Walker B, Bayley H. 1995. Key residues for membrane binding, oligomerization, and pore-forming activity of staphylococcal α-hemolysin identified by cysteine scanning mutagenesis and targeted chemical modification. J Biol Chem 270:23065-23071.
44. Walker B, Braha O, Cheley S, Bayley H. 1995. An intermediate in the assembly of a pore-forming protein trapped with a genetically-engineered switch. Chem Biol 2:99-105.
45. Walker BJ, Bayley H. 1994. A pore-forming protein with a protease-activated trigger. Protein Eng 7:91-97.
46. Walker BJ, Krishnasastry M, Zorn L, Kasianowicz JJ, Bayley H. 1992. Functional expression of the α-hemolysin of S. aureus in intact E. call and in cell lysates. J Biol Chem 267:10902-10909.