Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression

Molecular and General Genetics

Volumn 253, Issue 1-2, 1996, Pages 1-10

  Fujii, I ^a   Ono, Y ^a   Tada, H ^a   Gomi, K ^b   Ebizuka, Y ^a   Sankawa, U ^a,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

^c UNIVERSITY OF TOYAMA   (Japan)

Author keywords

6 methylsalicylic acid; 6 methylsalicylic acid synthase; Aspergillus terreus; Expression; Polyketide synthase gene

Indexed keywords

FUNGAL DNA; FUNGAL PROTEIN; POLYKETIDE; SALICYLIC ACID DERIVATIVE; SYNTHETASE;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIDULANS; ASPERGILLUS TERREUS; DNA FLANKING REGION; DNA LIBRARY; EXPRESSION VECTOR; FUNGAL GENETICS; GENE EXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT GENE; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; ASPERGILLUS; ASPERGILLUS NIDULANS; BASE SEQUENCE; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CLONING, MOLECULAR; GENE LIBRARY; GENES, FUNGAL; LIGASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MULTIENZYME COMPLEXES; OXIDOREDUCTASES; SALICYLIC ACIDS; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFORMATION, GENETIC;

ASPERGILLUS TERREUS; COLLETOTRICHUM LAGENARIUM; EMERICELLA NIDULANS; FUNGI; PENICILLIUM GRISEOFULVUM;

EID: 0029857563     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050289     Document Type: Article

References (46)

1. Beck J, Ripka S, Signer A, Schiltz E, Schweizer E (1990) The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Eur J Biochem 192:487-498
2. Bedford DJ, Schweizer E, Hopwood DA, Khosla C (1995) Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2). J Bacteriol 177:4544-4548
3. Bevitt DJ, Cortés J, Haydock SF, Leadlay PF (1992) 6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea. Eur J Biochem 204:39-49
4. Bibb MJ, Biró S, Motamedi H, Collins JF, Hutchinson CR (1989) Analysis of the nucleotide sequence of the Streptomyces glaucescens teml genes provides key information about the enzymology of polyketide antibiotic biosynthesis. EMBO J 8:2727-2736
5. Biel SW, Parrish FW (1986) Isolation DNA from fungal mycelia and sclerotia without the use of density gradient ultracentrifugation. Anal Biochem 154:21-25
6. Chargwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299
7. Chen ZG, Fujii I, Ebizuka Y, Sankawa U (1992) Emodin O-methyltransferase from Aspergillus terreus. Arch Microbiol 158:29-34
8. Chen ZG, Fujii I, Ebizuka Y, Sankawa U (1995) Emodinanthrone oxygenase from Aspergillua terreus. Phytochemistry 38:299-305
9. Cortes J.,Haydock SF, Roberts GA, Bevitt DJ, Leadlay PF (1990) An unusually large multifunctional polypeptide in the erythromycin-polyketide synthase of Succharopolyspora erythrea. Nature 346: 176-178
10. Dimroth P, Walter H, Lynen F (1970) Biosynthese von 6-methylsalicylsäure. Eur J Biochem 13:98-110
11. Donadio S, Katz L (1992) Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin biosynthesis in Saccharopolyspora erythrea. Gene 111:51-60
12. Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L (1991) Modular organization of genes required for complex polyketide biosynthesis. Science 252 : 675-679
13. Fincham JRS (1989) Transformation in fungi. Microbiol Rev 53:148-170
14. Fujii I, Ebizuka Y, Sankawa U (1982) Partial purification and some properties of emodin-O-methyltransferase from (+)-geodin producing strain of Aspegillus terreus. Chem Pharm Bull (Tokyo) 30:2283-2286
15. Fujii I, Iijima H, Ebizuka Y, Sankawa U (1983) Purification and characterization of dihydrogeodin oxidase from a fungal strain of Aspergillus terreus producing (+)-geodin. Chem Pharm Bull (Tokyo) 31:337-340
16. Fujii I, Iijima H, Tsukita S, Ebizuka Y, Sankawa U (1987) Purification and properties of dihydrogeodin oxidase from Aspergillus terreus. J Biochem 101:11-18
17. Fujii I, Ebizuka Y, Sankawa U (1988) A novel anthraquinone ring cleavage enzyme from Aspergillus terreus. J Biochem 103: 878-883
18. Fujii I, Chen ZG, Ebizuka Y, Sankawa U (1991) Identification of emodinanthrone oxygenase in fungus Aspergillus terreus. Biochem Int 25:1043-1049
19. 1 gene (rntA) from Aspergillus oryzae and its expression in Saccharomyces cererisiae and Aspergillus oryzae. Biosci Biotech Biochem 59:1869-1874
20. Gomi K, Iimura Y, Hara S (1987) Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene. Agric Biol Chem 51:2549-2555
21. Gurr S, Unkles SE, Kinghorn JR (1987) The structure and organization of nuclear genes of filamentous fungi. In: Kinghorn (ed) Gene structure in eukaryotic microbes. IRL Press, Oxford, pp 93-139
22. Hallam SE, Malpartida F, Hopwood DA (1988) DNA sequence, transcription and deduced function of a gene involved in polyketide antibiotic biosynthesis in Streptomyces coelicolor. Gene 74:305-320
23. Hopwood DA, Khosla C (1992) Genes for polyketide secondary metabolic pathways in microorganisms and plants. Ciba Found Symp 17:88-112
24. Hopwood DA, Sherman DH (1990) Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu Rev Genet 24:37-66
25. Huang KX, Fujii I, Ebizuka Y, Gomi K, Sankawa U (1995a) Molecular cloning and heterologous expression of the gene encoding dihydrogeodin oxidase, a multicopper blue enzyme from Anpergillus terreus. J Biol Chem 270:21495-21502
26. Huang KX, Iwakami N, Fujii I, Ebizuka Y, Sankawa U (1995b) Transformation of Penicillium islandicum and Penicilliium frequentans that produce anthraquinone-related compounds. Curr Genet 28:580-584
27. Hutchinson CR, Fujii I (1995) Polyketide synthase gene manipulation: a structure-function approach in engineering novel antibiotics. Annu Rev Microbiol 49:201-238
28. Malpartida F, Hallam SE, Kieser HM, Motamedi H, Hutchinson CR, Butler MJ, Sugden DA, Warren M, McKillop C, Bailey CR, Humphrey GO, Hopwood DA (1987) Homology between Streptomyces genes coding for synthesis of different polyketide used to clone antibiotic biosynthesis genes. Nature 325:818-821
29. Mayorga ME, Timberlake WE (1990) Isolation and molecular characterization of the Aspergillus nidulans wA gene. Genetics 126:73-79
30. Mayorga ME, Timberlake WE (1992) The developmentally regulated Aspergillus nidulans wA gene encodes a polypeptide homologous to polyketide and fatty acid synthases. Mol Gen Genet 235:205-212
31. O'Hagen D (1991) The polyketide metabolites. Ellis Horwood, NY
32. O'Hagen D (1992) Biosynthesis of polyketide metabolites. Nat Prod Rep 9:447-479
33. O'Hagen D (1993) Biosynthesis of fatty acid and polyketide metabolites. Nat Prod Rep 10:593-624
34. Pieper R, Luo G, Cane DE, Khosla C (1995) Cell-free synthesis of polyketides by recombinant erythromycin polyketide synthases. Nature 378:263-266
35. Pontecorvo P, Roper JA, Hammons LM, Macdonald KD, Bufton AW (1953) The genetics of Aspergillus nidulans. Adv Genet 5:141-238
36. Shen B, Hutchinson CR (1993) Enzymatic synthesis of a bacterial polyketide from acetyl and malonyl coenzyme A. Science 262:1535-1540
37. 2H] malonates. Biochemistry 31:9107-9116
38. Spencer JB, Jordan PM (1992b) Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum. Bioehem J 288:839-846
39. Tada S, Gomi K, Kitamoto K, Takahashi K, Tamura G, Hara S (1991) Construction of a fusion gene comprising the Takaamylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae. Mol Gen Genet 229:301-306
40. Takano Y, Kubo Y, Shimizu K, Mise K, Okuno T, Furusawa I (1995) Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis of Colletotrichum lagenarium. Mol Gen Genet 249:162-167
41. Tilburn J, Scazzocchia C, Taylor GG, Zabichy-Zissman JH, Lockington RA, Davies RW (1983) Transformation by integration in Aspergillus nidulans. Gene 26:205-221
42. Tsukamoto N, Fujii I, Ebizuka Y, Sankawa U (1992) Cloning of aklavinone biosynthesis genes from Streptomyces galilaeus. J Antibiot 45:1286-1294
43. Tsukamoto N, Fujii I, Ebizuka Y, Sankawa U (1994) Nucleotide sequence of the aknA region of the aklavinone biosynthesis gene cluster of Streptomyces galilaeus. J Bacteriol 176:2473-2475
44. Turner WB, Aldridge (1983) Fungal metabolites II. Academic Press, London
45. Woo ER, Fujii I, Ebizuka Y, Sankawa U, Kawaguchi A, Beale JM, Shibuya M, Mocek U, Floss HG (1989) Nonstereospecific proton removal in the enzymatic formation of orsellinic acid from chiral malonate. J Am Chem Soc 111:5498-5500
46. Yu JH, Leonard TJ (1995) Sterigmatocystin biosynthesis in Aspergillus nidulans requires a novel type I polyketide synthase. J Bacteriol 177:4792-4800