Crystal structure of a deletion mutant of human thymidylate synthase Δ(7-29) and its ternary complex with Tomudex and dUMP

Protein Science

Volumn 10, Issue 5, 2001, Pages 988-996

  Almog, R ^a   Waddling, C A ^a   Maley, F ^a   Maley, G F ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Crystal structure; dUMP; Ternary complex; Thymidylate synthase; Tomudex

Indexed keywords

DEOXYURIDINE PHOSPHATE; RALTITREXED; THYMIDYLATE SYNTHASE;

ARTICLE; COMPLEX FORMATION; CRYSTAL STRUCTURE; DRUG DESIGN; ENZYME ACTIVE SITE; ENZYME INHIBITOR INTERACTION; ENZYME SUBSTRATE COMPLEX; HUMAN; MOLECULAR INTERACTION; PRIORITY JOURNAL;

BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; DEOXYURACIL NUCLEOTIDES; DRUG DESIGN; ENZYME INHIBITORS; ESCHERICHIA COLI; HUMANS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; QUINAZOLINES; SEQUENCE DELETION; STRUCTURE-ACTIVITY RELATIONSHIP; THIOPHENES; THYMIDYLATE SYNTHASE;

ESCHERICHIA COLI;

EID: 0035061447     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.47601     Document Type: Article

References (29)

1. The structural mechanism for half-the-sites reactivity in an enzyme, thymidylate synthase, involves a relay of changes between subunits
2. The crystal structure of thymidylate synthase from Pneumocystis carinii reveals a fungal insert important for drug design
3. Trafficing of the C5 methylene intermediate in thymidylate synthase
4. Crystallography and NMR system: A new software suite for macromolecular structure determination
5. The catalytic mechanism and structure of thymidylate synthase
6. Collaborative Computing Project No. 4. The CCP4 suite: Programs for protein crystallography
7. SETOR: Hardware-highlighted three-dimensional solid model representation of macromolecules
8. Water-mediated substrate/product discrimination: The product complex of thymidylate synthase at 1.83 Å
9. Refined structure of substrate-bound and phosphate-bound thymidylate synthase from Lactobacillus casei
10. Crystal structure of thymidylate synthase A from Bacillus subtilis
11. Calculation of protein extinction coefficients from amino acid sequence data
12. Improved methods for the building of protein models in electron-density maps and the location of errors in these maps
13. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
14. PROCHECK: A program to check the stereochemical quality of protein structures
15. The purification and properties of thymidylate synthetase from chick embryo extract
16. Complete restoration of activity to inactive mutants of Escherichia coli thymidylate synthase: Evidence that E. Coli thymidylate synthase is a half-the-sites activity enzyme
17. Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2′-deoxyuridylate and 10-propargyl-5, 8-dideazafolate
18. Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate
19. AmoRe: An automated package for molecular replacement
20. Processing of X-ray diffraction data collected in oscillation mode
21. High-level expression of human thymidylate synthase
22. Plastic adaption toward mutations in proteins: Structural comparison of thymidylate synthases
23. Human thymidylate synthase is in the closed conformation when complexed with dUMP and Raltitrexed, an antifolate drug
24. Binding of the anticancer drug ZD1694 to E. Coli thymidylate synthase: Assessing specificity and affinity
25. An essential role for water in an enzyme reaction mechanism: The crystal structure of the thymidylate synthase mutant E58Q
26. Crystal structure of human thymidylate synthase: A structural mechanism for guiding substrates into the active site
27. Crystal structures of rat thymidylate synthase inhibited by Tomudex, a potent anticancer drug
28. Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis
29. Structure-based design of inhibitors specific for bacterial thymidylate synthase