Signal recognition particle mediated protein targeting in Escherichia coli

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 79, Issue 1, 2001, Pages 17-31

  Valent, Quido A ^a,b  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

Escherichia coli; Protein secretion; Protein targeting; Signal recognition particle

Indexed keywords

GUANINE NUCLEOTIDE; GUANOSINE TRIPHOSPHATE; MEMBRANE PROTEIN; RECEPTOR; RIBONUCLEOPROTEIN; SECRETORY PROTEIN; SIGNAL RECOGNITION PARTICLE;

AMINO ACID SEQUENCE; ARTICLE; CELL MEMBRANE; ENDOPLASMIC RETICULUM; ENZYME SPECIFICITY; ESCHERICHIA COLI; EUKARYOTE; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; PROTEIN INTERACTION; PROTEIN TARGETING; STRUCTURE ACTIVITY RELATION;

ENDOPLASMIC RETICULUM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EUKARYOTIC CELLS; MEMBRANE PROTEINS; PROTEIN TRANSPORT; SEQUENCE HOMOLOGY, AMINO ACID; SIGNAL RECOGNITION PARTICLE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA; PROKARYOTA;

EID: 0035034652     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010256109582     Document Type: Article

References (97)

1. The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase
2. Regulation by the ribosome of the GTPase of the signal-recognition particle during protein targeting
3. The primary pathway of protein export in E. coli
4. Alignment of conduits for the nascent polypeptide chain in the Ribosome-Sec61 complex
5. A two-step recognition of signal sequences determines the translocation efficiency of proteins
6. A common export pathway for proteins binding complex redox cofactors?
7. Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle
8. Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog
9. Protein targeting: Getting into the groove
10. The complete genome sequence of Escherichia coli K-12
11. The GTPase superfamily: A conserved switch for diverse cell functions
12. Translocation of proteins across the endoplasmic reticulum membrane
13. Enhancing effect of Bacillus subtilis Fth, a homologue of the SRP54 subunit of the mammalian signal recognition particle, on the binding of SecA to precursors of secretory proteins in vitro
14. The FtsQ protein of Escherichia coli: Membrane topology, abundance and cell division phenotypes due to overproduction and insertion mutations
15. The signal recognition particle-receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide
16. GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particle receptor
17. Requirement of GTP hydrolysis for dissociation of the signal recognition particle from its receptor
18. Leader peptidase
19. Directionality in protein translocation across membranes
20. Protein translocation into and across the bacterial plasma membrane and the plant thylakoid membrane
21. Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
22. Membrane association of FtsY, the E-coli SRP receptor
23. Kinetic partitioning-Potsing SecB to favor association with a rapidly tolding ligand
24. Titration of protein transport activity by incremental changes in signal peptide hydrophobicity
25. SecA, the peripheral pubunit of the Escherichia-coli precursor protein translocase is functional as a dimer
26. The Sec system
27. The SecDFyajC domain of preprotein translocase controls proprotein movement by regulating SecA membrane cycling
28. SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF
29. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
30. The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation
31. Structure of the conserved GTPase domain of the signal recognition particle
32. The identification of the Escherichia coli ftsY gene product: An unusual protein
33. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation
34. Hydrophobic region of signal peptides is a determinant for SRP recognition and protein translocation across the ER membrane
35. A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins
36. Membrane translocation of mitochondrially coded Cox2p: Distinct requirements for export of N and C termini and dependence on the conserved protein Oxalp
37. Oxalp mediates the export of the N- and C-termini of pCoxII from the mitochondrial matrix to the intermembrane space
38. Oxalp, an essential component of the N-tail protein export machinery in mitochondria
39. Chloroplast SRP54 interacts with a specific subset of thylakoid precursor proteins
40. Protein translocation at the ER membrane: A complex process becomes more so
41. A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane
42. Crystal structure of the signal sequence binding subunit of the signal recognition particle
43. Evidence for specificity at an early step in protein export in Escherichia coli
44. Highly selective binding of nascent polypeptides by an Escherichia coli chaperone protein in vivo
45. SeuB stabilizes a translocation-competent state of purified prePhoE protein
46. The functioning of the SRP receptor FtsY in protein-targeting in E. coli correlated with its ability to bind and hydrolyse GTP
47. Three pure chaperone proteins of Escherichia coli - SecB. trigger factor and GroEL-form soluble complexes with precursor proteins in vitro
48. Both lumenal and cytosolic gating of the aqueous ER translocon pore are regulated from inside the ribosome during membrane protein integration
49. Signal sequence recognition by an Escherichia coli ribonucleoprotein complex
50. An alternative protein targeting pathway in Escherichia coli: Studies on the role of FtsY
51. Signal recognition particle (SRP), a ubiquitous initiator of protein translocation
52. The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal-recognition particle
53. Interaction of E. coli Fth/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor
54. GTP binding and hydrolysis by the signal recognition particle during initiation of protein translocation
55. N-tail translocation of mature beta-lactamase across the Escherichia coli cytoplasmic membrane
56. Crystal structure of the NG domain from the signal-recognition particle receptor FtsY
57. The N-domain of the signal recognition particle 54-kDa subunit promotes efficient signal sequence binding
58. Signal sequences specify the targeting route to the endoplasmic reticulum membrane
59. Structure of a methionine-rich segment of Escherichia coli Fth protein
60. The E. coli fth gene is necessary for viability and efficient protein export
61. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
62. Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain
63. Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases
64. Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
65. Protein synthesis - A ribosome at the end of the tunnel
66. The complete general secretory pathway in gram-negative bacteria
67. SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle
68. Signal sequence recognition and targeting of ribosomes to the endoplasmic reticulum by the signal recognition particle do not require GTP
69. Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome nascent chain complexes to the endoplasmic reticulum
70. A bacterium catches up
71. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes
72. E. coli 4.5S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle
73. Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains
74. The 54-kD protein of signal recognition particle contains a methionine-rich RNA binding domain
75. The GTPase activity of the Escherichia coli Fth protein is important for normal growth
76. A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli
77. SecA is not required for signal recognition particle-mediated targeting and initial membrane insertion of a nascent inner membrane protein
78. Expression of gpsA encoding biosynthetic sn-glycerol 3-phosphate dehydrogenase suppresses both the LB-phenotype of a SecB null mutant and the cold-sensitive phenotype of a SecG null mutant
79. Elongation arrest is not a prerequisite for secretory protein translocation across the microsomal membrane
80. Functional dissection of the signal recognition particle
81. Protein secretion: Getting folded proteins across membranes
82. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
83. Early events in preprotein recognition in Escherichia coli interaction of SRP and trigger factor with nascent polypeptides
84. Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
85. The E. coli SRP and SecB targeting pathways converge at the translocon
86. The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events
87. The leader peptides from bacteriorhodopsin and halorhodopsin are potential membrane spanning amphipathic helices
88. Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane
89. A novel and ubiquitous system for membrane targeting and secretion of cofactor-containing proteins
90. Noncoordinate translation level regulation of ribosomal and nonribosomal protein genes in the Escherichia coli trmD operon
91. Protein translocation across the membrane of the endoplasmic reticulum
92. Evolutionary conserved nucleotides within the E. coli 4.5S RNA are required for association with P48 and for optimal function in vivo
93. An amino-terminal domain containing hydrophobic and hydrophilic sequences binds the signal recognition particle receptor α subunit to the β subunit on the endoplasmic reticulum membrane
94. The NG domain of the prokaryotic signal recognition particle receptor. FtsY, is fully functional when fused to an unrelated integral membrane polypeptide
95. The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains and RNA binding site and can be crosslinked to a signal sequence
96. GTPase domain of the 54-kD subunit of the mammalian signal recognition particle is required for protein translocation but not for signal sequence binding