Expression of gpsA encoding biosynthetic sn-glycerol 3-phosphate dehydrogenase suppresses both the LB- phenotype of a secB null mutant and the cold-sensitive phenotype of a secG null mutant

Molecular Microbiology

Volumn 26, Issue 5, 1997, Pages 1013-1021

  Shimizu, Hisayo ^a   Nishiyama, Ken Ichi ^a   Tokuda, Hajime ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOLIPID;

ARTICLE; BACTERIUM MUTANT; CELL MEMBRANE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; MEMBRANE COMPONENT; MEMBRANE STRUCTURE; NONHUMAN; PHOSPHOLIPID SYNTHESIS; PLASMID; PRIORITY JOURNAL; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030709234     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.6392003.x     Document Type: Article

References (58)

1. Altman, E., Kumamoto, C.A., and Emr, S.D. (1991) Heat-shock proteins can substitute for SecB function during protein export in Escherichia coli. EMBO J 10: 239-245.
2. Bell, R.M. (1974) Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol-3-phosphate acyltransferase Km mutant. J Bacteriol 117: 1065-1076.
3. Bell, R.M., and Cronan, J.E., Jr. (1975) Mutants of Escherichia coli defective in membrane phospholipid synthesis: phenotypic suppression of sn-glycerol-3-phosphate acyltransferase Km mutants by loss of feedback inhibition of the biosynthetic sn-glycerol-3-phosphate dehydrogenase. J Biol Chem 250: 7153-7158.
4. Bost, S., and Belin, D. (1995) A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery. EMBO J 14: 4412-4421.
5. Bost, S., and Belin, D. (1997) prl mutations in the Escherichia coli secG gene. J Biol Chem 272: 4087-4093.
6. Cabello, F., Timmis, K., and Cohen, S.N. (1976) Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature 259: 285-290.
7. Casadaban, M.J. (1976) Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage λ and μ. J Mol Biol 104: 541-555.
8. Clark, D., Lightner, V., Edgar, R., Modrich, P., Cronan, J.E., Jr, and Bell, R.M. (1980) Regulation of phospholipid biosynthesis in Escherichia coli: cloning of the structural gene for the biosynthetic sn-glycerol-3-phosphate dehydrogenase. J Biol Chem 255: 714-717.
9. Collier, D.N. (1993) SecB: a molecular chaperone of Escherichia coli protein secretion pathway. Adv Prot Chem 44: 151-193.
10. Collier, D.N., Bankaitis, V.A., Weiss, J.B., and Bassford, P.J., Jr. (1988) The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein. Cell 53: 273-283.
11. Cronan, J.E., Jr. (1978) Molecular biology of bacterial membrane lipids. Annu Rev Biochem 47: 163-189.
12. De Kruijff, B. (1994) Anionic phospholipids and protein translocation. FEBS Lett 346: 78-82.
13. Dittmer, J.C., and Lester, R.L. (1964) A simple, specific spray for the detection of phospholipids on thin layer chromatograms. J Lipid Res 5: 126-127.
14. Dowhan, W. (1997) Molecular basis for membrane phospholipid diversity: why are there so many lipids? Annu Rev Biochem 66: 199-232.
15. Driessen, A.J.M. (1994) How proteins cross the bacterial cytoplasmic membrane. J Membrane Biol 142: 145-159.
16. Duong, F., and Wickner, W. (1997) Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme. EMBO J 16: 2756-2768.
17. Economou, A., and Wickner, W. (1994) SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78: 835-843.
18. Economou, A., Pogliano, J.A., Beckwith, J., Oliver, D.B., and Wickner, W. (1995) SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF. Cell 83: 1171-1181.
19. Hanada, M., Nishiyama, K., and Tokuda, H. (1994) Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12). J Biol Chem 269: 23625-23631.
20. Hanada, M., Nishiyama, K., and Tokuda, H. (1996) SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low temperature. FEBS Lett 381: 25-28.
21. Hartl, F.-U., Lecker, S., Schiebel, E., Hendrick, J.P., and Wickner, W. (1990) The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane. Cell 63: 269-279.
22. Ito, K. (1996) The major pathways of protein translocation across membranes. Genes Cells 1: 337-346.
23. Kito, M., and Pizer, L.I. (1969) Purification and regulatory properties of the biosynthetic L-glycerol 3-phosphate dehydrogenase from Escherichia coli. J Biol Chem 244: 3316-3323.
24. Kohara, Y., Akiyama, K., and Isono, K. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50: 495-508.
25. Kontinen, V.P., and Tokuda, H. (1995) Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in a secG deletion mutant of Escherichia coli at low temperature. FEBS Lett 364: 157-160.
26. Kontinen, V.P., Helander, I.M., and Tokuda, H. (1996) The secG deletion mutation of Escherichia coli is suppressed by expression of a novel regulatory gene of Bacillus subtilis. FEBS Lett 389: 281-284.
27. Kumamoto, C.A. (1990) SecB protein: a cytoplasmic export factor that associates with nascent exported proteins. J Bioenerg Biomembr 22: 337-351.
28. Kumamoto, C.A., and Beckwith, J. (1983) Mutations in a new gene, secB, cause defective protein localization in Escherichia coli. J Bacteriol 154: 253-260.
29. Kumamoto, C.A., and Beckwith, J. (1985) Evidence for specificity at an early step in protein export in Escherichia coli. J Bacteriol 163: 267-274.
30. Kumamoto, C.A., and Nault, A.K. (1988) Characterization of the Escherichia coli protein-export gene secB. Gene 75: 167-175.
31. Kusters, R., De Vrije, T., Breukink, E., and de Kruijff, B. (1989) SecB protein stabilizes a translocation-competent state of purified prePhoE protein. J Biol Chem 264: 20827-27830.
32. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
33. Lecker, S.H., Driessen, A.J.M., and Wickner, W. (1990) ProOmpA contains secondary and tertiary structure prior to translocation and is shielded from aggregation by association with SecB protein. EMBO J 9: 2309-2314.
34. Lill, R., Dowhan, W., and Wickner, W. (1990) The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins. Cell 60: 271-280.
35. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with FoNn phenol reagent. J Biol Chem 193: 265-275.
36. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, p. 431.
37. Nishiyama, K., Hanada, M., and Tokuda, H. (1994) Disruption of the gene encoding p.12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature. EMBO J 13: 3272-3277.
38. Nishiyama, K., Mizushima., S., and Tokuda, H. (1992) The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli. J Biol Chem 267: 7170-7176.
39. Nishiyama, K., Mizushima, S., and Tokuda, H. (1993) A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli. EMBO J 12: 3409-3415.
40. Nishiyama, K., Suzuki, T., and Tokuda, H. (1996) Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell 85: 71-81.
41. Noda, A., Courtright, J.B., Denor, P.F., Webb, G., Kohara, Y., and Ishihama, A. (1991) Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones. Biotechniques 10: 474-477.
42. Pugsley, A.P. (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol Rev 57: 50-108.
43. Raetz, C.R.H. (1978) Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli. Microbiol Rev 42: 614-659.
44. Randall, L.L., Topping, T.B., and Hardy, S.J.S (1990) No specific recognition of leader peptide by SecB, a chaperone involved in protein export. Science 248: 860-863.
45. Ray, T.K., and Cronan, J.E., Jr. (1987) Acylation of glycerol-3-phosphate is the sole pathway of de novo phospholipid synthesis in Escherichia coli. J Bacteriol 169: 2896-2898.
46. Rietveld, A.G., Koorengevel, M.C., and de Kruijff, B. (1995) Non-bilayer lipids are required for efficient protein transport across the plasma membrane of Escherichia coli. EMBO J 14: 5506-5513.
47. Seoh, H.K., and Tai, P.C. (1997) Carbon source-dependent synthesis of SecB, a cytosolic chaperone involved in protein translocation across Escherichia coli membranes. J Bacteriol 179: 1077-1081.
48. Shibuya, I. (1992) Metabolic regulations and biological functions of phospholipids in Escherichia coli. Prog Lipid Res 31: 245-299.
49. Shibuya, I., Miyazaki, C., and Ohta, A. (1985) Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli. J Bacteriol 161: 1086-1092.
50. Shine, J., and Dalgarno, L. (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71: 1342-1346.
51. Sofia, H.J., Burland, V., Daniels, D.L., Plunkett III, G., and Blattner, F.R. (1994) Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 min. Nucleic Acids Res 22: 2576-2586.
52. Swidersky, U.E., Hoffschulte, H.K., and Muller, M. (1990) Determinants of membrane-targeting and transmembrane translocation during bacterial protein export. EMBO J 9: 1777-1785.
53. Tani, K., Tokuda, H., and Mizushima, S. (1990) Translocation of proOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. J Biol Chem 265: 17341-17347.
54. Tokuda, H. (1994) Biochemical characterization of the presecretory protein translocation machinery of Escherichia coli. FEBS Lett 346: 65-68.
55. Weiss, J.B., Ray, P.H., and Bassford, P.J., Jr. (1988) Purified SecB protein of Escherichia coli retards folding and promotes membrane translocation of maltose-binding protein in vitro. Proc Natl Acad Sci USA 88: 7903-7907.
56. Wickner, W., and Leonard, M.R. (1996) Escherichia coli preprotein translocase. J Biol Chem 271: 29514-29516.
57. Wild, J., Altman, E., Yura, T., and Gross, C.A. (1992) DnaK and DnaJ heat shock proteins participate in protein export in Escherichia Coli. Genes Dev 6: 1165-1172.
58. Wild, J., Walter, W.A., Gross, C.A., and Altman, E. (1993) Accumulation of secretory protein precursors in Escherichia coli induces the heat shock response. J Bacteriol 175: 3992-3997.