Interaction of a designed interleukin-10 epitope mimic with an antibody studied by isothermal titration microcalorimetry

Journal of Molecular Recognition

Volumn 14, Issue 2, 2001, Pages 89-98

  Welfle, Karin ^a   Misselwitz, Rolf ^a,c   Sabat, Robert ^b   Volk, Hans Dieter ^b   Schneider Mergener, Jens ^b,d   Reineke, Ulrich ^b,d   Welfle, Heinz ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d Jerini Bio Tools GmbH   (Germany)

Author keywords

Antigen antibody binding; Circular dichroism; Epitope mimic; Interleukin 10; Isothermal titration calorimetry; Miniprotein

Indexed keywords

ALPHA HELIX; BINDING AFFINITY; BINDING SITE; CALORIMETRY; CIRCULAR DICHROISM SPECTROSCOPY; EPITOPE; HEAT TREATMENT; INTERLEUKIN 10; ISOTHERMAL TITRATION CALORIMETRY; MOLECULAR RECOGNITION; MONOCLONAL ANTIBODY; PEPTIDE; TEMPERATURE; TRIFLUOROETHANOL;

ANTIGEN-ANTIBODY REACTIONS; BINDING SITES, ANTIBODY; BINDING, COMPETITIVE; CALORIMETRY; CIRCULAR DICHROISM; EPITOPES, B-LYMPHOCYTE; INTERLEUKIN-10; MOLECULAR MIMICRY; PEPTIDE LIBRARY;

EID: 0035020916     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.523     Document Type: Article

References (43)

1. Temperature dependence of the hydrophobic interaction in protein folding
2. Bound water molecules and conformational stabilization help mediate an antigen-antibody association
3. A conformationally homogeneous combinatorial peptide library
4. Minimizing a binding domain from protein A
5. Engineering antibodies for imaging and therapy
6. Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion
7. Determination of the helix and β form of proteins in aqueous solution by circular dichroism
8. The propagation of binding interactions to remote sites in proteins: Analysis of the binding of the monoclonal antibody D1.3 to lysozyme
9. Enthalpy and heat capacity changes for the proton dissociation of various buffer components in 0.1 M potassium chloride
10. Extraction of pharmacophore information from high-throughput screens
11. Uniquely folded mini-protein motifs
12. HER2 antibody Fab fragments
13. Constrained peptides as binding entities
14. Structure-based thermodynamic analysis of the dissociation of protein phosphatase-1 catalytic subunit and microcystin-LR docked complexes
15. Functional mimicry of a protein hormone by a peptide agonist: The EPO receptor complex at 2.8Å
16. The atomic structure of protein-protein recognition sites
17. Elucidating the folding problem of α-helical peptides using empirical parameters, II: Helix macropole effects and rational modification of the helical content of natural peptides
18. Elucidating the folding problem of α-helical peptides using empirical parameters III: Temperature and pH dependence
19. Development of the multiple sequence approximation within the Agadir model of α-helix formation. Comparison with Zimm-Bragg and Lifson-Roig formalisms
20. Solid model compounds and the thermodynamics of protein unfolding
21. Common features of protein unfolding and dissolution of hydrophobic compounds
22. Structural energetics of peptide recognition: Angiotensin II/antibody binding
23. Entropy in biological binding processes: Estimation of translational entropy loss
24. Configurational effects in antibody-antigen interactions studied by microcalorimetry
25. Measuring the conformational stability of a protein
26. Conformational stability and thermodynamics of folding of ribonucleases Sa, Sa2 and Sa3
27. A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric study
28. A set of constructed type spectra for the practical estimation of peptide secondary structure from circular dichroism
29. A synthetic mimic of a discontinuous binding site on interleukin-10
30. Neutralizing murine monoclonal anti-interleukin-10 antibodies enhance binding of antibodies against a different epitope
31. Parameters of helix-coil transition theory for alanine-based peptides of varying chain lengths in water
32. Coupling of local folding to site-specific binding of proteins to DNA
33. Protein-peptide interactions
34. Novel miniproteins engineered by the transfer of active sites to small natural scaffolds
35. Engineering novel proteins by transfer of active sites to natural scaffolds
36. Conformation, pH-induced conformational changes, and thermal unfolding of anti-p24 (HIV-1) monoclonal antibody CB4-1 and its Fab and Fc fragments
37. A ten-residue fragment of an antibody (mini-antibody) directed against lysozyme as ligand in immunoaffinity chromatography
38. Peptide and cyclopeptide libraries: Automated synthesis, analysis and receptor binding assays
39. Rapid measurement of binding constants and heats of binding using a new titration calorimeter
40. Small peptides as potent mimetics of the protein hormone erythropoietin