-
1
-
-
0022386929
-
Changing the binding specificity of a repressor by redesigning an ex-helix
-
Wharton RP, Ptashne M: Changing the binding specificity of a repressor by redesigning an ex-helix. Nature 1985, 316:601-605.
-
(1985)
Nature
, vol.316
, pp. 601-605
-
-
Wharton, R.P.1
Ptashne, M.2
-
2
-
-
0022558297
-
Replacing the complementarity-determining regions in a human antibody with those from a mouse
-
Jones PT, Dear PH, Foote J, Neuberger MS, Winter G: Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature 1986, 321:522-525.
-
(1986)
Nature
, vol.321
, pp. 522-525
-
-
Jones, P.T.1
Dear, P.H.2
Foote, J.3
Neuberger, M.S.4
Winter, G.5
-
3
-
-
0026520387
-
Converting trypsin to chymotrypsin: The role of surface loops
-
Hedstrom L, Szilagyi L, Rutter W: Converting trypsin to chymotrypsin: the role of surface loops. Science 1992, 255:1249-1253.
-
(1992)
Science
, vol.255
, pp. 1249-1253
-
-
Hedstrom, L.1
Szilagyi, L.2
Rutter, W.3
-
4
-
-
0030600761
-
Attempts to convert chymotrypsin to trypsin
-
Venekei I, Szilagyi L, Gràf L, Rutter W: Attempts to convert chymotrypsin to trypsin. FEBS Lett 1996, 379:143-147.
-
(1996)
FEBS Lett
, vol.379
, pp. 143-147
-
-
Venekei, I.1
Szilagyi, L.2
Gràf, L.3
Rutter, W.4
-
5
-
-
0023394374
-
Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering
-
Wells JA, Cunningham BC, Graycar TP, Estell DA: Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering. Proc Natl Acad Sci USA 1987, 84:5167-5171.
-
(1987)
Proc Natl Acad Sci USA
, vol.84
, pp. 5167-5171
-
-
Wells, J.A.1
Cunningham, B.C.2
Graycar, T.P.3
Estell, D.A.4
-
6
-
-
0028874551
-
Designing subtilisin BPN′ to cleave substrates containing dibasic residues
-
Ballinger MD, Tom J, Wells JA: Designing subtilisin BPN′ to cleave substrates containing dibasic residues. Biochemistry 1995, 34:13312-13319.
-
(1995)
Biochemistry
, vol.34
, pp. 13312-13319
-
-
Ballinger, M.D.1
Tom, J.2
Wells, J.A.3
-
7
-
-
0029913564
-
Furilisin: A variant of subtilisin BPN′ engineered for cleaving tribasic substrates
-
Ballinger MD, Tom J, Wells JA: Furilisin: a variant of subtilisin BPN′ engineered for cleaving tribasic substrates. Biochemistry 1996, 35:13579-13585. One of the most successful 'transfers' of specificity from a eukaryotic protease to a structurally homologous bacterial protease. The engineered protease can be of practical utility in protein processing and is easily expressed in E. coli, in contrast to the eukaryotic one.
-
(1996)
Biochemistry
, vol.35
, pp. 13579-13585
-
-
Ballinger, M.D.1
Tom, J.2
Wells, J.A.3
-
8
-
-
0027190966
-
Structural differences between wild-type NADP-dependent glutathione reductase from Escherichia coli and a redesigned NAD-dependent mutant
-
Mittl PRE, Berry A, Scrutton NS, Perham RN, Schultz GE: Structural differences between wild-type NADP-dependent glutathione reductase from Escherichia coli and a redesigned NAD-dependent mutant J Mol Biol 1993, 231:191-195.
-
(1993)
J Mol Biol
, vol.231
, pp. 191-195
-
-
Mittl, P.R.E.1
Berry, A.2
Scrutton, N.S.3
Perham, R.N.4
Schultz, G.E.5
-
9
-
-
0025019734
-
Redesigning of the coenzyme specificity of a dehydrogenase by protein engineering
-
Scrutton NS, Berry A, Perham RN: Redesigning of the coenzyme specificity of a dehydrogenase by protein engineering. Nature 1990, 343:38-43.
-
(1990)
Nature
, vol.343
, pp. 38-43
-
-
Scrutton, N.S.1
Berry, A.2
Perham, R.N.3
-
10
-
-
0027480616
-
Creation of an NADP-dependant pyruvate dehydrogenase multienzyme complex by protein engineering
-
Bocanegra JA, Scruton NS, Perham RN: Creation of an NADP-dependant pyruvate dehydrogenase multienzyme complex by protein engineering. Biochemistry 1993, 32:2737-2740.
-
(1993)
Biochemistry
, vol.32
, pp. 2737-2740
-
-
Bocanegra, J.A.1
Scruton, N.S.2
Perham, R.N.3
-
11
-
-
0030002725
-
Redesigning secondary structure to invert coenzyme specificity in isopropylmalate dehydrogenase
-
Chen R, Greer A, Dean AM: Redesigning secondary structure to invert coenzyme specificity in isopropylmalate dehydrogenase. Proc Natl Acad Sci USA 1996, 93:12171-12176. A remarkable example of intervention in an enzyme active site, showing that rational engineering of secondary structures to produce enzymes with novel properties is feasible.
-
(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 12171-12176
-
-
Chen, R.1
Greer, A.2
Dean, A.M.3
-
12
-
-
0027401045
-
Use of a zinc-finger consensus sequence framework and specificity rules to design specific DNA binding proteins
-
Desjarlais JR, Berg JM: Use of a zinc-finger consensus sequence framework and specificity rules to design specific DNA binding proteins. Proc Natl Acad Sci USA 1993, 90:2256-2260.
-
(1993)
Proc Natl Acad Sci USA
, vol.90
, pp. 2256-2260
-
-
Desjarlais, J.R.1
Berg, J.M.2
-
13
-
-
0029102175
-
Phage display: Protein engineering by direct evolution
-
O'Neil KT, Hoess RH: Phage display: protein engineering by direct evolution. Curr Opin Struct Biol 1995, 5:443-449.
-
(1995)
Curr Opin Struct Biol
, vol.5
, pp. 443-449
-
-
O'Neil, K.T.1
Hoess, R.H.2
-
14
-
-
0025214021
-
Engineering human prolactin to bind to the human growth hormone receptor
-
Cunningham BC, Henner DJ, Wells JA: Engineering human prolactin to bind to the human growth hormone receptor. Science 1990, 247:1461-1465.
-
(1990)
Science
, vol.247
, pp. 1461-1465
-
-
Cunningham, B.C.1
Henner, D.J.2
Wells, J.A.3
-
15
-
-
0024580993
-
A covalent angiogenin/ribonuclease hybrid with a fourth disulfide bond generated by regional mutagenesis
-
Harper JW, Vallee BL: A covalent angiogenin/ribonuclease hybrid with a fourth disulfide bond generated by regional mutagenesis. Biochemistry 1989, 28:1875-1884.
-
(1989)
Biochemistry
, vol.28
, pp. 1875-1884
-
-
Harper, J.W.1
Vallee, B.L.2
-
16
-
-
0029092077
-
Replacing a surface loop endows ribonuclease A with angiogenic activity
-
Raines RT, Toscano MTP, Nierengarten DM, Ha JH, Auerbach R: Replacing a surface loop endows ribonuclease A with angiogenic activity. J Biol Chem 1995, 270:17180-17184.
-
(1995)
J Biol Chem
, vol.270
, pp. 17180-17184
-
-
Raines, R.T.1
Toscano, M.T.P.2
Nierengarten, D.M.3
Ha, J.H.4
Auerbach, R.5
-
17
-
-
0028960069
-
Engineering of fibroblast growth factor: Alteration of receptor binding specificity
-
Seddon AP, Aviezer D, Li L-Y, Böhlen P, Yayon A: Engineering of fibroblast growth factor: alteration of receptor binding specificity. Biochemistry 1995, 34:731-736.
-
(1995)
Biochemistry
, vol.34
, pp. 731-736
-
-
Seddon, A.P.1
Aviezer, D.2
Li, L.-Y.3
Böhlen, P.4
Yayon, A.5
-
18
-
-
0024558027
-
Immune response to cholera toxin epitope inserted in Salmonella flagellin
-
Newton SMC, Jacob CO, Stocker BAD: Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science 1989, 244:70-72.
-
(1989)
Science
, vol.244
, pp. 70-72
-
-
Newton, S.M.C.1
Jacob, C.O.2
Stocker, B.A.D.3
-
19
-
-
0024595598
-
Fimbriae of Bacteroides nodosus: Protein engineering of the structural subunit for the production of an exogenous peptide
-
Jennings PA, Bills MM, Irving DO, Mattrick JS: Fimbriae of Bacteroides nodosus: protein engineering of the structural subunit for the production of an exogenous peptide. Protein Eng 1989, 2:365-369.
-
(1989)
Protein Eng
, vol.2
, pp. 365-369
-
-
Jennings, P.A.1
Bills, M.M.2
Irving, D.O.3
Mattrick, J.S.4
-
20
-
-
14744281085
-
A genetic system to elicit and monitor anti-peptide antibodies without peptide synthesis
-
Martineau P, Charbit A, Leclerc C, Werts C, O'Callaghan D, Hofnung M: A genetic system to elicit and monitor anti-peptide antibodies without peptide synthesis. Biotechnology 1991, 9:170-172.
-
(1991)
Biotechnology
, vol.9
, pp. 170-172
-
-
Martineau, P.1
Charbit, A.2
Leclerc, C.3
Werts, C.4
O'Callaghan, D.5
Hofnung, M.6
-
21
-
-
0027129063
-
Antigenized antibodies
-
Zanetti M: Antigenized antibodies. Nature 1992, 355:476-477.
-
(1992)
Nature
, vol.355
, pp. 476-477
-
-
Zanetti, M.1
-
22
-
-
0027398099
-
Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule
-
Zaghouani H, Steinman R, Nonacs R, Shah H, Gerhard W, Bona C: Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule. Science 1993, 259:224-227.
-
(1993)
Science
, vol.259
, pp. 224-227
-
-
Zaghouani, H.1
Steinman, R.2
Nonacs, R.3
Shah, H.4
Gerhard, W.5
Bona, C.6
-
23
-
-
0027191391
-
Structural and functional analyses of the Agr-Gly-Asp sequence introduced into human lysozyme
-
Yamada T, Matsushima M, Inaka K, Ohkubo T, Uyeda A, Maeda T, Titani K, Sekiguchi K, Kikuchi M: Structural and functional analyses of the Agr-Gly-Asp sequence introduced into human lysozyme. J Biol Chem 1993, 268:10588-10592.
-
(1993)
J Biol Chem
, vol.268
, pp. 10588-10592
-
-
Yamada, T.1
Matsushima, M.2
Inaka, K.3
Ohkubo, T.4
Uyeda, A.5
Maeda, T.6
Titani, K.7
Sekiguchi, K.8
Kikuchi, M.9
-
24
-
-
0028135311
-
Functional analysis and modeling of a conformationally constrained Arg-Gly-Asp sequence inserted into human lysozyme
-
Yamada T, Uyeda A, Kidera A, Kikuchi M: Functional analysis and modeling of a conformationally constrained Arg-Gly-Asp sequence inserted into human lysozyme. Biochemistry 1994, 33:11678-11683.
-
(1994)
Biochemistry
, vol.33
, pp. 11678-11683
-
-
Yamada, T.1
Uyeda, A.2
Kidera, A.3
Kikuchi, M.4
-
25
-
-
0028947622
-
Structure of a conformationally constrained Arg-Gly-Asp sequence inserted into human lysozyme
-
Yamada T, Song H, Inaka K, Shimada Y, Kikuchi M, Matsushima M: Structure of a conformationally constrained Arg-Gly-Asp sequence inserted into human lysozyme. J Biol Chem 1995, 270:5687-5690.
-
(1995)
J Biol Chem
, vol.270
, pp. 5687-5690
-
-
Yamada, T.1
Song, H.2
Inaka, K.3
Shimada, Y.4
Kikuchi, M.5
Matsushima, M.6
-
26
-
-
0027446694
-
3 by RGD sequences installed into a presentation scaffold
-
3 by RGD sequences installed into a presentation scaffold. Protein Eng 1993, 6:745-754.
-
(1993)
Protein Eng
, vol.6
, pp. 745-754
-
-
Lee, G.1
Chan, W.2
Hurle, M.R.3
DesJarlais, R.L.4
Watson, F.5
Sathe, G.M.6
Wetzel, R.7
-
27
-
-
0027485980
-
Expression of conformationally constrained adhesion peptide in an antibody CDR loop and inhibition of natural killer cell cytotoxic activity by an antibody antigenized with the RGD motif
-
Zanetti M, Filaci G, Lee RH, del Guercio P, Rossi F, Bacchetta R, Stevenson F, Barnaba V, Billetta R: Expression of conformationally constrained adhesion peptide in an antibody CDR loop and inhibition of natural killer cell cytotoxic activity by an antibody antigenized with the RGD motif. EMBO J 1993, 12:4375-4384.
-
(1993)
EMBO J
, vol.12
, pp. 4375-4384
-
-
Zanetti, M.1
Filaci, G.2
Lee, R.H.3
Del Guercio, P.4
Rossi, F.5
Bacchetta, R.6
Stevenson, F.7
Barnaba, V.8
Billetta, R.9
-
28
-
-
0028884260
-
A paradigm for drug discovery using a conformation from the crystal structure of a presentation scaffold
-
Zhao B, Helms LR, DesJarlais RL, Abdel-Meguid SS, Wetzel R: A paradigm for drug discovery using a conformation from the crystal structure of a presentation scaffold. Nat Struct Biol 1995, 2:1131-1137.
-
(1995)
Nat Struct Biol
, vol.2
, pp. 1131-1137
-
-
Zhao, B.1
Helms, L.R.2
DesJarlais, R.L.3
Abdel-Meguid, S.S.4
Wetzel, R.5
-
29
-
-
0029059477
-
Incorporation of glutamine repeats makes protein oligomerize: Implication for neurodegenerative diseases
-
Stott K, Blackburn JM, Butler PJG, Perutz M: Incorporation of glutamine repeats makes protein oligomerize: implication for neurodegenerative diseases. Proc Natl Acad Sci USA 1995, 92:6509-6513.
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 6509-6513
-
-
Stott, K.1
Blackburn, J.M.2
Butler, P.J.G.3
Perutz, M.4
-
30
-
-
0024556397
-
Transfer of a β-turn structure to a new protein context
-
Hynes TR, Kautz RA, Goodman MA, Gill JF, Fox RO: Transfer of a β-turn structure to a new protein context Nature 1989, 339:73-76.
-
(1989)
Nature
, vol.339
, pp. 73-76
-
-
Hynes, T.R.1
Kautz, R.A.2
Goodman, M.A.3
Gill, J.F.4
Fox, R.O.5
-
31
-
-
0027288623
-
Modularity of protein function: Chimeric interleukin 1β containing specific protease inhibitor loops retains function of both molecules
-
Wolfson AJ, Kanaoka M, Lau F, Ringe D, Young P, Lee J, Blumenthal J: Modularity of protein function: chimeric interleukin 1β containing specific protease inhibitor loops retains function of both molecules. Biochemistry 1993, 32:5327-5331.
-
(1993)
Biochemistry
, vol.32
, pp. 5327-5331
-
-
Wolfson, A.J.1
Kanaoka, M.2
Lau, F.3
Ringe, D.4
Young, P.5
Lee, J.6
Blumenthal, J.7
-
32
-
-
0030007648
-
Changing the structural context of a functional β-hairpin. Synthesis and characterisation of a chimera containing the curar emimetic loop of a snake toxin in the scorpion α/β scaffold
-
Drakopoulou E, Zinn-Justin S, Guenneugues M, Gilquin B, Ménez A, Vita C: Changing the structural context of a functional β-hairpin. Synthesis and characterisation of a chimera containing the curar emimetic loop of a snake toxin in the scorpion α/β scaffold. J Biol Chem 1996, 271:11979-11987. This work, together with [33], clearly indicates that the scorpion toxin α/α scaffold is quite permissive for sequence mutations and stable even after multiple substitutions. It represents a very interesting host structure to display, within a well defined structural context, solvent-exposed β hairpin and/or α helical motifs from structurally unrelated but biologically relevant proteins. The transfer operation to this stable fold may result in novel proteins presenting useful properties as ligands (antagonists and agonists) of membrane receptors and/or as inhibitors of protein-protein interacrions.
-
(1996)
J Biol Chem
, vol.271
, pp. 11979-11987
-
-
Drakopoulou, E.1
Zinn-Justin, S.2
Guenneugues, M.3
Gilquin, B.4
Ménez, A.5
Vita, C.6
-
33
-
-
0029050766
-
Scorpion toxins as natural scaffold for protein engineering
-
Vita C, Roumestand C, Toma F, Ménez A: Scorpion toxins as natural scaffold for protein engineering. Proc Natl Acad Sci USA 1995, 92:6404-6408.
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 6404-6408
-
-
Vita, C.1
Roumestand, C.2
Toma, F.3
Ménez, A.4
-
34
-
-
0029895602
-
Transfer of a β-hairpin from the functional site of snake curaremimetic toxins to the α/β scaffold of scorpion toxins: Three-dimensional solution structure of the chimeric protein
-
Zinn-Justin S, Guenneugues M, Drakopoulou E, Gilquin B, Vita C, Ménez A: Transfer of a β-hairpin from the functional site of snake curaremimetic toxins to the α/β scaffold of scorpion toxins: three-dimensional solution structure of the chimeric protein. Biochemistry 1996, 35:8535-8543.
-
(1996)
Biochemistry
, vol.35
, pp. 8535-8543
-
-
Zinn-Justin, S.1
Guenneugues, M.2
Drakopoulou, E.3
Gilquin, B.4
Vita, C.5
Ménez, A.6
-
35
-
-
0029584214
-
IIbβ3
-
IIbβ3. J Biol Chem 1996, 270:30486-30490. A remarkable success in the transfer of an active loop to a new structural scaffold. The new hybrid protein binds to the platelet integrin with nanomolar affinity. Stimulating perspectives in combining the loop transfer approach with the phage display methodology are disclosed.
-
(1996)
J Biol Chem
, vol.270
, pp. 30486-30490
-
-
Smith, J.W.1
Tachias, K.2
Madison, E.L.3
-
36
-
-
0028568028
-
Building synthetic antibodies as adhesive ligands for integrins
-
Smith JW, Hu D, Satterthwait A, Pinz-Sweeny S, Barbas CF: Building synthetic antibodies as adhesive ligands for integrins. J Biol Chem 1994, 269:32788-32795.
-
(1994)
J Biol Chem
, vol.269
, pp. 32788-32795
-
-
Smith, J.W.1
Hu, D.2
Satterthwait, A.3
Pinz-Sweeny, S.4
Barbas, C.F.5
|