A synthetic mimic of a discontinuous binding site on interleukin-10

Nature Biotechnology

Volumn 17, Issue 3, 1999, Pages 271-275

  Reineke, Ulrich ^a,c   Sabat, Robert ^a   Misselwitz, Rolf ^b,d   Welfle, Heinz ^d   Volk, Hans Dieter ^a   Schneider Mergener, Jens ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^c Jerini Bio Tools GmbH   (Germany)

^d MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Antibody epitope; Interleukin 10; Mini protein; Peptide library; Protein mimics

Indexed keywords

DISULFIDE; EPITOPE; INTERLEUKIN 10;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING SITE; GENE MAPPING; PEPTIDE ANALYSIS; PRIORITY JOURNAL; RECEPTOR AFFINITY; RECEPTOR BINDING; RECEPTOR INTRINSIC ACTIVITY; RECEPTOR SENSITIVITY;

AMINO ACID SUBSTITUTION; BINDING SITES; BINDING, COMPETITIVE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HUMANS; INTERLEUKIN-10; KINETICS; LEUKOCYTES, MONONUCLEAR; LIPOPOLYSACCHARIDES; PEPTIDE BIOSYNTHESIS; PEPTIDE MAPPING; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0033037586     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/7018     Document Type: Article

References (27)

1. Peters, K.P., Fauck, J. & Frömmel, C. The automatic search for ligand binding sites in proteins of known three-dimensional structure using only geometric criteria. J. Mol. Biol. 256, 201-213 (1996).
2. Tian, S.-S. et al. A small, nonpeptidyl mimic of granulocyte-colony-stimulating factor. Science 281, 257-259 (1998).
3. Ballinger, M.D. & Wells, J.A. Will any dimer do? Nat. Struct. Biol. 5, 938-940 (1998).
4. Li, B. et al. Minimization of a polypeptide hormone. Science 270, 1657-1660 (1995).
5. Braisted, A.C. & Wells, J.A. Minimizing a binding domain from protein A. Proc. Natl. Acad. Sci. USA 93, 5688-5692 (1996).
6. Wrighton, N.C. et al. Small peptides as potent mimetics of the protein hormone erythropoietin. Science 273, 458-463 (1996).
7. Reineke, U. & Schneider-Mergener, J. Protein mimicry: a new dimension for peptides as lead compounds? Angew. Chem. Int. Edit. Engl. 37, 769-771 (1998).
8. Wrighton, N.C. et al. Increased potency of an erythropoietin peptide mimetic through covalent dimerization. Nat. Biotechnol. 15, 1261-1265 (1997).
9. Geysen, H.M., Rodda, S.J. & Mason, T.J. A priori delineation of a peptide which mimics a discontinuous antigenic determinant. Mol. Immunol. 23, 709-715 (1986).
10. Zhang, X. et al. Synthetic CD4 exocyclics inhibit binding of human immunodeficiency virus type 1 envelope to CD4 and virus replication in T lymphocytes. Nat. Biotechnol. 15, 150-154 (1997).
11. Takasaki, W., Kajino, Y., Kajino, K., Murali, R. & Greene, M.I. Structure-based design and characterization of exocyclic peptidomimetics that inhibit TNFα binding to its receptor. Nat. Biotechnol. 15, 1266-1270 (1997).
12. Reineke, U., Sabat, R., Volk, H.D. & Schneider-Mergener, J. Mapping of the interleukin-10/interleukin-10 receptor combining site. Protein Sci. 7, 951-960 (1998).
13. Atassi, M.Z. & Zablocki, W. Can an antibody-combining site be mimicked synthetically? J. Biol. Chem. 252, 8784-8787 (1977).
14. Frank, R. Spot synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48, 9217-9232 (1992).
15. Kramer, A. et al. Combinatorial cellulose-bound peptide libraries: screening tools for the identification of peptides that bind ligands with predefined specificity. METHODS (Comp. Meth. Enzymol.) 6, 388-395 (1994).
16. Frank, R. et al. in Combinatorial Peptide and Nonpeptide Libraries (ed. Jung, G.) 363-386 (Wiley-VCH Verlag, Weinheim, Germany, 1996).
17. Kramer, A. & Schneider-Mergener, J. Synthesis and screening of peptide libraries on continuous cellulose membrane supports. Methods. Mol. Biol. 87, 25-39 (1998).
18. Sabat, R., Seifert, M., Volk, H.D. & Glaser, R.W. Neutralizing murine monoclonal anti-interleukin-10 antibodies enhance binding of antibodies against a different epitope. Mol. Immunol. 33, 1103-1111 (1996).
19. Rüdiger, S., Germeroth, L., Schneider-Mergener, J. & Bukau, B. Substrate specificity of the DnaK chaperone determined by screening cellulose-bound peptide libraries. EMBO J. 16, 1501-1507 (1997).
20. Zdanov, A., Schalk-Hihi, C. & Wlodawer, A. Crystal structure of human interleukin-10 at 1.6 Å resolution and a model of a complex with its soluble receptor. Protein Sci. 5, 1955-1962. (1996).
21. Kramer, A. et al. Molecular base for the binding promiscuity of a monoclonal anti-p24(HIV-1) antibody. Cell 91, 799-809 (1997).
22. Tam, J.P., Wu, C.-R., Liu, W. & Zhang, J.-W. Disulfide bond formation in peptides by dimethyl sulfoxide. Scope and applications. J. Am. Chem. Soc. 113, 6657-6662 (1991).
23. Kotenko, S.V. et al. Identification and functional characterization of a second chain of the interleukin-10 receptor complex. EMBO J. 16, 5894-5903 (1997).
24. Zdanov, A., Schalk-Hihi, C., Menon, S., Moore, K.W. & Wlodawer, A. Crystal structure of Epstein-Barr virus protein BCRF1, a homolog of cellular interleukin-10. J. Mol. Biol. 268, 460-467 (1997).
25. Malin, R. et al. Identification of technetium-99m binding peptides using combinatorial cellulose-bound peptide libraries. J. Am. Chem. Soc. 117, 11821-11822 (1995).
26. Reineke, U. et al. Mapping protein-protein contact sites using cellulose-bound peptide scans. Mol. Divers. 1, 141-148 (1996).
27. Friguet, B., Chaffotte, A.F., Djavadi-Ohaniance, L. & Goldberg, M.E. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods 77, 305-319 (1985).