Ras-induced colony formation and anchorage-independent growth inhibited by elevated expression of Purα in NIH3T3 cells

Journal of Cellular Biochemistry

Volumn 81, Issue 4, 2001, Pages 621-638

  Barr, Sharon M ^a   Johnson, Edward M ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

CDK; Colony formation; Cyclin; Focus formation; PEST sequence; PURA; Ras

Indexed keywords

CYCLIN A; CYCLIN B1; CYCLIN D1; CYCLIN DEPENDENT KINASE 2; PROTEIN PUR ALPHA; RNA BINDING PROTEIN; SINGLE STRANDED DNA BINDING PROTEIN; UNCLASSIFIED DRUG;

ANCHORAGE INDEPENDENT GROWTH; ANIMAL CELL; ARTICLE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CELL STRAIN 3T3; COLONY FORMATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DELETION MUTANT; GROWTH INHIBITION; MOUSE; NONHUMAN; ONCOGENE K RAS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION;

3T3 CELLS; AMINO ACID MOTIFS; ANALYSIS OF VARIANCE; ANIMALS; CELL CYCLE; CELL DIVISION; CELL NUCLEUS; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; CYCLIN B; DNA-BINDING PROTEINS; GENE EXPRESSION; MICE; MUTATION; NERVE TISSUE PROTEINS; RAS PROTEINS; TRANSCRIPTION FACTORS; TRANSFORMATION, GENETIC;

EID: 0035003585     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcb.1099     Document Type: Article

References (62)

1. Ras links growth factor signaling to the cell cycle machinery via regulation of cyclin D1 and the Cdk inhibitor p27KIP1
2. Transforming p21ras mutants and c-Ets-2 activate the cyclin D1 promoter through distinguishable regions
3. Increased expression of cyclin D1 and the Rb tumor suppressor gene in c-K-ras transformed rat enterocytes
4. The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication
5. Sequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein
6. Reversal of terminal differentiation and control of DNA replication: Cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication
7. Evidence that replication of human neurotropic JC virus DNA in glial cells is regulated by the sequence-specific single-stranded DNA-binding protein Pur alpha
8. The single-stranded DNA binding protein, Pur-alpha, binds HIV-1 TAR RNA and activates HIV-1 transcription
9. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges
10. Expression and subcellular localization of cyclin D1 protein in epithelial ovarian tumour cells
11. Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization
12. Functional interactions of the retinoblastoma protein with mammalian D- type cyclins
13. K-ras modulates the cell cycle via both positive and negative regulatory pathways
14. Induction of cyclin D1 overexpression by activated ras
15. Self-association of Puralpha is mediated by RNA
16. SURVEY AND SUMMARY: Puralpha: A multifunctional single-stranded DNA- and RNA-binding protein
17. A 39-kD DNA-binding protein from mouse brain stimulates transcription of myelin basic protein gene in oligodendrocytic cells
18. MPF localization is controlled by nuclear export
19. V-src-induced-transcription of the avian clusterin gene
20. RNA-dependent DNA binding activity of the Pur factor, potentially involved in DNA replication and gene transcription
21. Alterations in Pur alpha levels and intracellular localization in the CV-1 cell cycle
22. Association of human Pur alpha with the retinoblastoma protein, Rb, regulates binding to the single-stranded DNA Pur alpha recognition element
23. Early nuclear events in the induction of lymphocyte proliferation by mitogens. Effects of concanavalin A on the phosphorylation and distribution of non-histone chromatin proteins
24. Ras induces anchorage-independent growth by subverting multiple adhesion-regulated cell cycle events
25. Differential phosphorylation and turnover of nuclear acidic proteins during the cell cycle of synchronized HeLa cells
26. Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4
27. Induction of p27Kip1 degradation and anchorage independence by Ras through the MAP kinase signaling pathway
28. The single-stranded DNA-binding proteins, puralpha, purbeta, and MSY1 specifically interact with an exon 3-derived mouse vascular smooth muscle alpha-actin messenger RNA sequence
29. Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Puralpha and Purbeta
30. Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated intermediates
31. Host control of HIV-1 parasitism in T cells by the nuclear factor of activated T cells
32. Activation of the JC virus Tat-responsive transcriptional control element by association of the Tat protein of human immunodeficiency virus 1 with cellular protein Pur alpha
33. Regulation of the human fas promoter by YB-1, Puralpha and AP-1 transcription factors
34. Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases
35. The PURA gene, encoding the single-stranded-DNA-binding protein Pur. Alpha as a marker for 5q31 alteration in myeloproliferative disorders, a potential early step in induction of AML
36. Casein kinase 2 binds and phosphorylates the nucleosome assembly protein-1 (NAP1) in Drosophila melanogaster
37. Ras transformation results in an elevated level of cyclin D1 and acceleration of G1 progression in NIH 3T3 cells
38. Localization of PURA, the gene encoding the sequence-specific single- stranded-DNA-binding protein Pur alpha, to chromosome band 5q31
39. A series of mammalian expression vectors and characterization of their expression of a reporter gene in stably and transiently transfected cells
40. Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells
41. Characterization of single-stranded cAMP response element binding protein (ssCRE-BP) from mouse cerebellum
42. Involvement of a single-stranded DNA binding protein, ssCRE-BP/Pur alpha, in morphine dependence
43. Expression of cyclin D1 and retinoblastoma protein in colorectal cancer
44. Production of high-titer helper-free retroviruses by transient transfection
45. Clinical and prognostic implications of chromosome 5q deletions: 96 high resolution studied patients
46. Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1
47. The KEN box: An APC recognition signal distinct from the D box targeted by Cdh1
48. Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport
49. Microtubules containing acetylated alpha-tubulin in mammalian cells in culture
50. Overexpression of mouse D-type cyclins accelerates G1 phase in rodent fibroblasts
51. PEST sequences and regulation by proteolysis
52. The transcriptional effect of WT1 is modulated by choice of expression vector
53. Ectopic expression of cyclin D1 but not cyclin E induces anchorage-independent cell cycle progression
54. Inhibitors of mammalian G1 cyclin-dependent kinases
55. Redistribution of U-snRNPs during mitosis
56. Cell cycle arrest and morphological alterations following microinjection of NIH3T3 cells with Pur alpha
57. Association of JC virus large T antigen with myelin basic protein transcription factor (MEF-1/Puralpha) in hypomyelinated brains of mice transgenically expressing T antigen
58. The retinoblastoma protein and cell cycle control
59. Regulation of the cell cycle machinery by oncogenic ras
60. Interaction of HIV- Tat with Pur-alpha in nuclei of human glial cells: Characterization of RNA-mediated protein-protein binding
61. Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1
62. DNA-binding protein Pur alpha and transcription factor YY1 function as transcription activators of the neuron-specific FE65 gene promoter