The amino acid residues affecting the activity and azole susceptibility of rat CYP51 (sterol 14-demethylase P450)

Journal of Biochemistry

Volumn 129, Issue 5, 2001, Pages 761-768

  Nitahara, Yuko ^a   Kishimoto, Kae ^b   Yabusaki, Yoshiyasu ^b   Gotoh, Osamu ^c   Yoshida, Yuzo ^d   Horiuchi, Tadao ^a   Aoyama, Yuri ^a  

^a SOKA UNIVERSITY   (Japan)

^b SUMITOMO CHEMICAL CO LTD   (Japan)

^c SAITAMA CANCER CENTER   (Japan)

^d MUKOGAWA WOMEN'S UNIVERSITY   (Japan)

Author keywords

Azole antifungal agents; CYP51; Moleculer modeling; Point mutation; Sterol 14 demethylase P450

Indexed keywords

AMINO ACID; ANTIFUNGAL AGENT; KETOCONAZOLE; PYRROLE DERIVATIVE; STEROL 14ALPHA DEMETHYLASE; THREONINE;

AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG PROTEIN BINDING; DRUG SENSITIVITY; ESCHERICHIA COLI; MOLECULAR MODEL; MUTANT; NONHUMAN; POINT MUTATION; PROTEIN DEGRADATION; PROTEIN STRUCTURE; RAT;

AMINO ACID SUBSTITUTION; ANIMALS; AZOLES; CATALYSIS; COMPUTER SIMULATION; CONSERVED SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME ACTIVATION; ISOLEUCINE; MODELS, MOLECULAR; OXIDOREDUCTASES; POINT MUTATION; PROTEIN STRUCTURE, TERTIARY; RATS; SEQUENCE ALIGNMENT;

ANIMALIA; ESCHERICHIA COLI;

EID: 0034992094     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002917     Document Type: Article

References (49)

1. 14DMand by intact microsomes
2. 14DM)
3. Sterol biosynthesis
4. 14DM) is one of the most ancient and conserved P450 species
5. Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: II. Evolutionary analysis of protein and gene structures
6. Sterol 14-demethylase P450 (CYP51) provides a breakthrough for the discussion on the evolution of cytochrome P450 gene superfamily
7. 14DM) of Saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol
8. Properties and structural requirements for substrate specificity of cytochrome P-450-dependent obtusifoliol 14α-demethylase from maize (Zea mays) seedlings
9. 14DM) of yeast: Difference between the substrate recognition by yeast and plant sterol 14α-demethylase
10. Purification and characterization of rat sterol 14-demethylase P450 (CYP51) expressed in Escherichia coli
11. Biochemical target for antifungal azole derivatives: Hypothesis on the mode of action
12. 14DMpurified from Saccharomyces cerevisiae microsomes
13. Mode of action studies. Basis of the search of new antifungal drugs
14. Effects of buthiobate, a fungiside, on cytochrome P-450 of rat liver microsomes
15. Interaction of azole derivatives with cytochrome P-450 isozymes in yeast, fungi, plants and mammalian cells
16. 14DM) of yeast
17. Cytochrome P-450 of fungi: Primary target for azole antifungal agents
18. 14DMfrom yeast
19. Some properties of a detergent-solubilized NADPH-cytochrome c (cytochrome P-450) reductase purified by biospecific affinity chromatography
20. The carbon monooxide-binding pigment of liver microsomes: I. Evidence for its hemoprotein nature
21. Protein measurement with the folin phenol reagent
22. Molecular modeling of rat sterol 14-demethylase P450 (CYP51)
23. BM-3, a prototype for microsomal P450's
24. Significant improvement in accuracy of multiple protein sequence alignments by iterative refinement as assessed by reference to structural alignments
25. High-regulation crystal structure of cytochrome P-450cam
26. Crystal structure and refinement of cytochrome P450terp at 2.3 Å resolution
27. Structure of cytochrome P450eryF involved in erythromycin biosynthesis
28. Crystal structure of nitric oxide reductase from denitrifying fungus Fusarium oxysporum
29. Evolution, structure, and gene regulation of cytochrome P-450
30. Evolution and differentiation of P450 genes
31. Nitrogenous ligation at the sixth coordination position of the Thr-301 to Lys-mutated P450IIC2 heme iron
32. Molecular properties and reaction mechanisms
33. Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: Possible role of the hydroxy amino acid in oxygen activation
34. Crystal structure of the cytochrome P-450cam active site mutant Thr252Ala
35. Proton and electron transfer mechanism in dioxygen activation by cytochrome P450cam
36. A role of Asp-251 in cytochrome P-450cam oxygen activation
37. Role of Thr-252 in cytochrome P450cam: A study with unnatural amino acid mutagenesis
38. Understanding the role of the essential Asp251 in cytochrome p450cam using site-directed mutagenesis, crystallography, and kinetic solvent isotope effect
39. Role of Glu318 at the putative distal site in the catalytic function of cytochrome P450d
40. The P450 superfamily: A group of versatile hemoproteins contributing to the oxidation of various small molecules
41. A model study on aromatase cytochrome P450 reaction: Transformation of 4-androsten-19-al-3,17-dione to 10β-hydroxyetr-4-ene-3,17-dione
42. A site-directed mutagenesis study of human placental aromatase
43. Functional domains of human aromatase cytochrome P450 characterized by linear alignment and site-directed mutagenesis
44. 14DM(lanosterol 14-demethylase)
45. The mutation T315A in Candida albicans sterol 14α-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity
46. 14DM) and 7-ethoxycoumarin O-deethylase P450 of rat liver microsomes
47. Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450
48. Characterization and catalytic properties of the sterol 14α-demethylase from Mycobacterium tuberculosis
49. CYP51-like gene of Mycobacterium tuberculosis actually encodes a P450 similar to eukaryotic CYP51