Transgene expression in the brain stem effected by intramuscular injection of polyethylenimine/DNA complexes

Molecular Therapy

Volumn 3, Issue 5, 2001, Pages 658-664

  Wang, Shu ^a   Ma, Nan ^a   Gao, Shujun J ^a   Yu, Hanry ^a   Leong, Kam W ^a,b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b Johns Hopkins University   (United States)

Author keywords

Endocytosis; Gene transfer; Hypoglossal system; Motor neuron diseases; Polyethylenimine; Retrograde axonal transport

Indexed keywords

CHITOSAN; LIPID; LUCIFERASE; PLASMID DNA; POLYETHYLENEIMINE; POLYLYSINE; PROTEIN BCL 2; VIRUS VECTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLOOD BRAIN BARRIER; BRAIN STEM; COMPLEX FORMATION; CYTOMEGALOVIRUS; DNA DETERMINATION; DNA VECTOR; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENE TRANSFER; GENETIC TRANSFECTION; HYPOGLOSSAL NERVE; INTERNALIZATION; MALE; MOTONEURON; NERVE ENDING; NERVE FIBER; NONHUMAN; PERIKARYON; POLYMERASE CHAIN REACTION; PROMOTER REGION; RAT; REPORTER GENE; ROUS SARCOMA ONCOVIRUS; TONGUE; TRANSGENE; WESTERN BLOTTING;

ANIMALIA; CYTOMEGALOVIRUS; ONCOVIRINAE; ROUS SARCOMA VIRUS;

EID: 0034990933     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2001.0324     Document Type: Article

References (30)

1. Gene therapy in the CNS
2. A latent, nonpathogenic HSV-1-derived vector stably expresses β-galactosidase in mouse neurons
3. Specific and efficient gene transfer strategy offers new potentialities for the treatment of motor neuron diseases
4. CNS gene delivery by retrograde transport of recombinant replication-defective adenoviruses
5. Characterization of adenoviral gene expression in spinal cord after remote vector delivery
6. Targeting and gene expression in spinal cord motor neurons following intramuscular inoculation of an HSV-1 vector
7. Herpes simplex virus 1-mediated transfer of preproenkephalin A in rat dorsal root ganglia
8. Antihyperalgesic effects of infection with a preproenkephalin-encoding herpes virus
9. Development and optimization of herpes simplex virus vectors for multiple long-term gene delivery to the peripheral nervous system
10. Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector
11. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
12. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: Polyethylenimine
13. 2+ channel structure and function with antisense oligonucleotides
14. Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system
15. Recent techniques for tracing pathways in the central nervous system of developing and adult mammals
16. DNA-polycation nanospheres as non-viral gene delivery vehicles
17. Bcl-2 expression regulates cell sensitivity to S100b-mediated apoptosis
18. A simple quantitative dot-immunobinding assay for glial and neuronal marker proteins in SDS-solubilized brain tissue extracts
19. Fibre composition of the hypoglossal nerve in the rat
20. Determinants of in vivo MR imaging of slow axonal transport
21. Transport of cytoskeletal elements in the squid giant axon
22. Ionization of poly(ethylenimine) and poly(allylamine) at various pH's
23. Quantitative aspects of drug and toxicant-induced astrogliosis
24. Utilization of the herpes simplex virus type 1 latency-associated regulatory region to drive stable reporter gene expression in the nervous system
25. Cellular and humoral immune responses to viral antigens create barriers to lung-directed gene therapy with recombinant adenoviruses
26. Gene delivery to spinal motor neurons
27. Axon-mediated gene transfer of retinal ganglion cell in vivo
28. Poly(ethylenimine)-mediated transfection: A new paradigm for gene delivery
29. Axonal transport and the neuronal cytoskeleton
30. Prolonged in vivo gene expression driven by a tyrosine hydroxylase promoter in a defective herpes simplex virus amplicon vector