The multi-structural feature of the multidrug resistance gene product p-glycoprotein: Implications for its mechanism of action (Hypothesis)

Molecular Membrane Biology

Volumn 18, Issue 2, 2001, Pages 145-152

  Zhang, J T ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

ABC transporter; Biogenesis; Chemotherapy; Conformational changes; Multidrug resistance; P glycoprotein; Topology

Indexed keywords

ABC TRANSPORTER; ANTINEOPLASTIC AGENT; GENE PRODUCT; GLYCOPROTEIN P;

ARTICLE; DRUG TRANSPORT; GENE STRUCTURE; GENE TRANSFER; HYDROPHOBICITY; MULTIGENE FAMILY; PRIORITY JOURNAL; PROTEIN FOLDING; REGULATORY MECHANISM;

EID: 0034926792     PISSN: 09687688     EISSN: None     Source Type: Journal    
DOI: 10.1080/09687680110048831     Document Type: Article

References (74)

1. Multidrug resistance protein (Mdr)-alkaline phosphatase hybrids in Escherichia coli suggest a major revision in the topology of the C-terminal half of Mdr
2. Membrane topology of multidrug resistance protein expressed in Escherichia coli. N-terminal domain
3. Intracellular protein topogenesis
4. Biogenesis of polytopic membrane proteins: Membrane segments assemble within translocation channels prior to membrane integration
5. Two different regions of P-glycoprotein are photoaffinity-labeled by azidopine
6. mdr2 encodes P-glycoprotein expressed in the bile canalicular membrane as determined by isoform-specific antibodies
7. Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells
8. Identification of a sister gene to P-glycoprotein
9. An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene
10. P-glycoprotein epitope mapping. I. Identification of a linear human-specific epitope in the fourth loop of the P-glycoprotein extracellular domain by MM4.17 murine monoclonal antibody to human multi-drug-resistant cells
11. Localization of the iodomycin binding site in hamster P-glycoprotein
12. ATP-dependent transport systems in bacteria and humans: Relevance to cystic fibrosis and multidrug resistance
13. Topology of P-glycoprotein as determined by epitope mapping of MRK-16 monoclonal antibody
14. Homology between P-glycoprotein and a bacterial haemolysin transport protein suggests a model for multidrug resistance
15. Biochemistry of multidrug resistance mediated by the multidrug transporter
16. Major photoaffinity drug labeling sites for iodoaryl azidoprazosin in P-glycoprotein are within or immediately C-terminal to, transmembrane domains 6 and 12
17. Mammalian multidrug resistance gene: Complete cDNA sequence indicates strong homology to bacterial transport proteins
18. Mechanism involved in generating the carboxyl-terminal half topology of P-glycoprotein
19. Membrane orientation of carboxyl-terminal half P-glycoprotein: Topogenesis of transmembrane segments
20. Predicting the orientation of eukaryotic membrane-spanning proteins
21. ABC transporters: From microorganisms to man
22. A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants
23. P-glycoprotein: Multidrug-resistance and a superfamily of membrane-associated transport proteins
24. Membrane topology of P-glycoprotein as determined by epitope insertion: Transmembrane organization of the N-terminal domain of mdr3
25. Transmembrane organization of mouse P-glycoprotein determined by epitope insertion and immunofluorescence
26. An inventory of the human ABC proteins
27. Structural requirements for membrane assembly of proteins spanning the membrane several times
28. Site-directed fluorescence labeling of P-glycoprotein on cysteine residues in the nucleotide binding domains
29. Fluorescence studies on the nucleotide binding domains of the P-glycoprotein multidrug transporter
30. Proximity of the nucleotide binding domains of the P-glycoprotein multidrug transporter to the membrane surface: A resonance energy transfer study
31. Intrinsic fluorescence of the P-glycoprotein multidrug transporter: Sensitivity of tryptophan residues to binding of drugs and nucleotides
32. Functional consequences of phenylalanine mutations in the predicted transmembrane domain of P-glycoprotein
33. Functional consequences of proline mutations in the predicted transmembrane domain of P-glycoprotein
34. Membrane topology of a cysteine-less mutant of human P-glycoprotein
35. Identification of residues in the drug-binding site of human P-glycoprotein using a thiol-reactive substrate
36. The glycosylation and orientation in the membrane of the third cytoplasmic loop of human P-glycoprotein is affected by mutations and substrates
37. Identification of residues in the drug-binding domain of human P-glycoprotein. Analysis of transmembrane segment 11 by cysteine-scanning mutagenesis and inhibition by dibromobimane
38. Identification of residues within the drug-binding domain of the human multidrug resistance P-glycoprotein by cysteine-scanning mutagenesis and reaction with dibromobimane
39. P-glycoprotein function involves conformational transitions detectable by differential immunoreactivity
40. Localization of the forskolin labeling sites to both halves of P-glycoprotein: Similarity of the sites labeled by forskolin and prazosin
41. Identification of members of the P-glycoprotein multigene family
42. Transmembrane aromatic amino acid distribution in P-glycoprotein
43. Stability and covalent modification of P-glycoprotein in multidrug-resistant KB cells
44. Lower electrical membrane potential and altered pHi homeostasis in multidrug-resistant (MDR) cells: Further characterization of a series of MDR cell lines expressing different levels of P-glycoprotein
45. Epitope mapping of the monoclonal antibody MM12.10 to external MDR1 P-glycoprotein domain by synthetic peptide scanning and phage display technologies
46. Structure of the multidrug resistance P-glycoprotein to 2.5 nm resolution determined by electron microscopy and image analysis
47. Phosphatidylcholine translocase: A physiological role for the mdr2 gene
48. A general pattern for substrate recognition by P-glycoprotein
49. The catalytic cycle of P-glycoprotein
50. P-glycoprotein-mediated Hoechst 33342 transport out of the lipid bilayer
51. Characterization and epitope mapping of several new anti-P-glycoprotein monoclonal antibodies
52. Insights into the structure and substrate interactions of the P-glycoprotein multidrug transporter from spectroscopic studies
53. Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli
54. Amino-terminal assembly of human P-glycoprotein at the endoplasmic reticulum is directed by cooperative actions of two internal sequences
55. Transmembrane orientation and topogenesis of the third and fourth membrane-spanning regions of human P-glycoprotein (MDR1)
56. Evidence for an alternate model of human P-glycoprotein structure and biogenesis
57. Homozygous disruption of the murine mdr2 P-glycoprotein gene leads to a complete absence of phospholipid from bile and to liver disease
58. Secondary and tertiary structure changes of reconstituted P-glycoprotein. A Fourier transform attenuated total reflection infrared spectroscopy analysis
59. Ligand-mediated tertiary structure changes of reconstituted P-glycoprotein. A tryptophan fluorescence quenching analysis
60. Expression of a full-length cDNA for the human 'MDR1' gene confers resistance to colchicine, doxorubicin, and vinblastine
61. Role of ribosomes in reinitiation of membrane insertion of intemal transmembrane segments in a polytopic membrane protein
62. Dissection of drug-binding-induced conformational changes in P-glycoprotein
63. Conformational changes of P-glycoprotein by nucleotide binding
64. Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence
65. Cytoplasmic orientation and two-domain structure of the multidrug transporter, P-glycoprotein, demonstrated with sequence-specific antibodies
66. Study of membrane orientation and glycosylated extracellular loops of mouse P-glycoprotein by in vitro translation
67. Membrane orientation of transmembrane segments 11 and 12 of MDR- and non-MDR-associated P-glycoproteins
68. Involvement of cytoplasmic factors regulating the membrane orientation of P-glycoprotein sequences
69. Dissection of de novo membrane insertion activities of internal transmembrane segments of ATP-binding-cassette transporters: Toward understanding topological rules for membrane assembly of polytopic membrane proteins
70. Membrane topology of the N-terminal half of the hamster P-glycoprotein molecule
71. Role of the ribosome in sequence-specific regulation of membrane targeting and translocation of P-glycoprotein signal-anchor transmembrane segments
72. Topological determinants of internal transmembrane segments in P-glycoprotein sequences
73. Topological folding and proteolysis profile of P-glycoprotein in membranes of multidrug-resistant cells: Implications for the drug-transport mechanism