The structure of apo protein-tyrosine phosphatase 1b c215S mutant: More than just an S → O change

Protein Science

Volumn 10, Issue 8, 2001, Pages 1596-1605

  Scapin, Giovanna ^a   Patel, Sangita ^a   Patel, Vira ^b   Kennedy, Brian ^b   Asante Appiah, Ernest ^b  

^a MERCK RESEARCH LABORATORIES   (United States)

^b MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Conformational change; Crystal structure; Loop flexibility; Substrate trapping mutant

Indexed keywords

APOPROTEIN; PHOSPHATASE; PHOSPHOTYROSINE; PROTEIN TYROSINE PHOSPHATASE;

ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; COVALENT BOND; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME CONFORMATION; HYDROGEN BOND; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CALORIMETRY; CRYSTALLOGRAPHY, X-RAY; HUMANS; LIGANDS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN-TYROSINE-PHOSPHATASE; THERMODYNAMICS;

EID: 0034926053     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.11001     Document Type: Article

References (53)

1. Cross-validated maximum likelihood enhances crystallographic simulated annealing refinement
2. 2-(oxalylamino)-benzoic acid is a general, competitive inhibitor of protein-tyrosine phosphatases
3. Crystal structure of human protein tyrosine phosphatase 1B
4. Protein phosphatases
5. Probing the combining site of an anti-carbohydrate antibody by saturation-mutagenesis: Role of the heavy-chain CDR3 residues
6. The free R value: A novel statistical quantity for assessing the accuracy of crystal structures
7. Crystallography and NMR system: A new software suite for macromolecular structure determination
8. Ribbons
9. The CCP4 suite: Programs for protein crystallography
10. Isolation and structural elucidation of a novel phosphocysteine intermediate in the LAR protein tyrosine phosphatase enzymatic pathway
11. Stabilization of charges and protonation studies in the active site of protein tyrosine phosphatases: A computational study
12. The x-ray crystal structure of Yersinia tyrosine phosphatase with bound tungstate and nitrate
13. X-ray crystal structure and characterization of halide-binding sites of human myeloperoxidase at 1.8 Å Resolution
14. Protein tyrosine phosphatases: A diverse family of intracellular and transmembrane enzymes
15. Development of "substrate-trapping" mutants to identify physiological substrates of protein tyrosine phosphatases
16. Hydrogen bonds involving sulfur atoms in proteins
17. Structural basis for inhibition of the protein tyrosine phosphatase 1B by phosphotyrosine peptide mimetics
18. Evidence for protein-tyrosine-phosphatase catalysis proceeding via a cysteine-phosphate intermediate
19. Nature of the transition state of the protein-tyrosine phosphatase-catalyzed reaction
20. Does positive charge at the active sites of phosphatases cause a change in mechanism? The effect of the conserved arginine on the transition state for phosphoryl transfer in the protein-tyrosine phosphatase from Yersinia
21. Data processing in macromolecular crystallography
22. Protein kinases and phosphatases: The yin and yang of protein phosphorylation and signaling
23. Effects of substitutions of closely related amino acids at the contact surface in an antigen-antibody complex on thermodynamic parameters
24. Structure-based design of a low molecular weight, nonphosphorus, nonpeptide, and highly selective inhibitor of protein-tyrosine phosphatase 1B
25. Structural basis for phosphotyrosine peptide recognition by protein tyrosine phosphatase 1B
26. Electron-density map interpretation
27. Rapid loop dynamics of Yersinia protein-tyrosine phosphatase
28. Thermodynamic analysis of an antibody functional epitope
29. Checking your imagination: Applications of the free R value
30. PROCHECK: A programme to check the stereochemical quality of protein structure coordinates
31. Location of bromide ions in tetragonal lysozyme Crystals
32. Roles of aspartic acid-181 and serine-222 in intermediate formation and hydrolysis of the mammalian protein-tyrosine-phosphatase PTP1
33. Enthalpy-entropy compensation phenomena in water solutions of proteins and small molecules: A ubiquitous property of water
34. Solvent content of protein crystals
35. AmoRe: An automated package for molecular replacement
36. Protein tyrosine phosphatases in signal transduction
37. Processing of x-ray diffraction data collected in oscillation mode
38. Visualization of the cysteinyl-phosphate intermediate of a protein tyrosine-phosphatase by x-ray crystallography
39. Improved structure refinement through maximum likelihood
40. The covalent and tertiary structure of bovine liver rhodanese
41. Identification of a second aryl phosphate-binding site in protein tyrosine phosphatase 1B: A paradigm for inhibitor design
42. Torsion angle dynamics: Reduced variable conformational sampling enhances crystallographic structure refinement
43. Molecular basis for the dephosphorylation of the activation segment of the insulin receptor by protein tyrosine phosphatase 1B
44. Structural basis of plasticity in protein tyrosine phosphatase 1b substrate recognition
45. A ligand induced conformational change in the Yersinia protein tyrosine phosphatase
46. Crystal structure of the Yersinia protein tyrosine phosphatase at 2.5 Å and the complex with tungstate
47. Crystal structure of Escherichia coli xanthine phosphoribosyltransferase
48. Conformational and dynamic changes of Yersinia protein tyrosine phosphatase induced by ligand binding and active site mutation and revealed by H/D exchange and electrospray ionization fourier transform ion cyclotron resonance mass spectrometry
49. Active site labelling of the Yersinia protein tyrosine phosphatase: The determination of the pKa of the active site cysteine and the function of the conserved histidine 402
50. The Cys(X)5Arg catalytic motif in phosphoester hydrolysis
51. Dissecting the catalytic mechanism of protein-tyrosine phosphatases
52. The single sulfur to oxygen substitution in the active site nucleofile of the Yersinia protein tyrosine phosphatase leads to substantial structural and functional perturbation
53. Thermodynamic study of ligand binding to protein-tyrosine phosphatase 1B and its substrate-trapping mutants