Rapid quantitation of gene therapy specific CFTR expression using the amplification refractory mutation system

BioTechniques

Volumn 27, Issue 1, 1999, Pages 122-127

  Thorpe, Peter H ^a   Porteous, David J ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSMEMBRANE CONDUCTANCE REGULATOR;

ARTICLE; DNA TEMPLATE; GENE AMPLIFICATION; GENE EXPRESSION; GENE MUTATION; GENE THERAPY; HUMAN; POLYMERASE CHAIN REACTION; TRANSGENE;

EID: 0033019734     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99271st05     Document Type: Article

References (15)

1. Alton, E.W.F.W., D.M. Geddes, D.R. Gill, C.F. Higgins, S.C. Hyde, J.A. Innes and D.J. Porteous. 1998. Towards gene therapy for cystic fibrosis: a clinical progress report. Gene Ther. 5:291-292.
2. Bi, W. and P.J. Stambrook. 1998. Detection of known mutation by proof-reading PCR. Nucleic Acids Res. 26:3073-3075.
3. Davies, J.C., D.M. Geddes and E.W.F.W. Alton. 1998. Prospects for gene therapy for cystic fibrosis. Mol. Med. Today 4:292-299.
4. Ehlen, T. and L. Dubeau. 1989. Detection of ras point mutations by polymerase chain reaction using mutation specific, inosin-containing oligonucleotide primers. Biochem. Biophys. Res. Commun. 160:441-447.
5. Gregory, R.J., S.H. Cheng, D.P. Rich, J. Marshall, S. Paul, K. Hehir, L. Ostedgaard, K.W. Klinger, M.J. Welsh and A.E. Smith. 1990. Expression and characterization of the cystic fibrosis transmembrane regulator. Nature 347:382-386.
6. Hart, S.L., E. Mayall, M. Stern, F.M. Munkonge, A. Frost, L. Huang, M. Vasilliou, R. Williamson, E.W.F.W. Alton and C. Coutelle. 1995. The introduction of two silent mutations into a CFTR cDNA construct allows improved detection of exogenous mRNA in gene transfer experiments. Hum. Mol. Genet. 4:1597-1602.
7. Huang, M.M., N. Amheim and M.F. Goodman. 1992. Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR. Nucleic Acids Res. 20:4567-4573.
8. Kwok, S., D.E. Kellogg, N. McKinney, D. Spasic, L. Goda, C. Levenson and J.J. Sninsky. 1990. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type I model studies. Nucleic Acids Res. 18:999-1005.
9. Lockey, C., E. Otto and Z. Long. Real-time fluorescence detection of a single DNA molecule. BioTechniques 24:744-746.
10. Mayall, E.S. and C. Coutelle. 1997. RT-PCR method specific for the detection of transgenic CFTR mRNA in the presence of transgene plasmid DNA and endogenous CFTR mRNA. Gene Ther. 4:875-878.
11. Newton, C.R., A. Graham, L.E. Heptinstall, S.J. Powell, C. Summers, N. Kalsheker, J.C. Smith and A.F. Markham. 1989. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 17:2503-2516.
12. Okayama, H., D.T. Curiel, M.L. Brantly, M.D. Holmes and R.G. Crystal. 1989. Rapid, nonradioactive detection of mutations in the human genome by allele-specific amplification. J. Lab. Clin. Med. 114:105-113.
13. Ugozzoli, L. and R.B. Wallace. 1991. Allele-specific polymerase chain reaction. Methods: A Companion to Methods Enzymol. 2:42-48.
14. Wittwer, C.T., K.M. Ririe, R.V. Andrew, D.A. David, R.A. Gundry and U.J. Balis. 1997. The LightCycler™: a microvolume multisample fluorimeter with rapid temperature control. BioTechniques 22:176-181.
15. Wu, D.Y., L. Ugozzoli, B.J. Pal and R.B. Wallace. 1989. Allele-specific enzymatic amplification of β-globin genomic DNA for diagnosis of sickle cell anemia. Proc. Natl. Acad. Sci. USA 86:2757-2760.