Co-expression of human cytochrome P4501A1 (CYP1A1) variants and human NADPH-cytochrome P450 reductase in the baculovirus/insect cell system

Xenobiotica

Volumn 31, Issue 6, 2001, Pages 345-356

  Schwarz, D ^a   Kisselev, P ^a   Honeck, H ^a   Cascorbi, I ^a   Schunck, W H ^a   Roots, I ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BENZO[A]PYRENE; CYTOCHROME P450 1A1; ETHOXYRESORUFIN DEETHYLASE; GENE PRODUCT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; DNA POLYMORPHISM; ELECTRON TRANSPORT; ENZYME ACTIVITY; HUMAN; INSECT; MICROSOME; MOLECULAR STABILITY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; TRANSLATION REGULATION;

ANIMALS; BACULOVIRIDAE; BENZO(A)PYRENE; CARCINOGENS; CYTOCHROME P-450 CYP1A1; GENE EXPRESSION; GENETIC VECTORS; HUMANS; KINETICS; MICROSOMES; MUTAGENESIS; NADPH-FERRIHEMOPROTEIN REDUCTASE; SPODOPTERA; SUBSTRATE SPECIFICITY; TRANSFECTION;

ANIMALIA; INSECTA; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS;

EID: 0034913574     PISSN: 00498254     EISSN: None     Source Type: Journal    
DOI: 10.1080/00498250110055947     Document Type: Article

References (31)

1. Oxidation of benzo[a]pyrene by recombinant human cytochrome P450 enzymes
2. Inherent specificities of purified cytochromes P450 and P448 toward biphenyl hydroxylation and ethoxyresorufin deethylation
3. cDNA-directed expression of human cytochrome P450 CYP1A1 using baculovirus
4. A C4887A polymorphism in exon 7 of human CYP1A1: Population frequency, mutation linkages, and impact on lung cancer susceptibility
5. Coexpression of cytochrome P4502A6 and human NADPH-P450 oxidoreductase in the baculovirus system
6. Contribution of human cytochrome P450 to benzo[a]pyrene and benzo[a]pyrene-7,8-dihydrodiol metabolism, as predicted from heterologous expression in yeast
7. Cytochromes P450 expression systems
8. Activation of procarcinogens by human cytochrome P450 enzymes
9. Genetic linkage of lung cancer-associated MspI polymorphisms with amino acid replacement in the heme binding region of the human cytochrome P450IA1 gene
10. Effects of inducers and epoxide hydrolase on the metabolism of benzo[a]pyrene by liver microsomes and a reconstituted system: Analysis by high-pressure liquid chromatography
11. CYP1A1 polymorphisms and lung cancer risk: A meta-analysis
12. Human cytochrome P450 (CYP) genes: Recommendations for the nomenclature of alleles
13. 1-450: Complementary DNA and amino acid sequence
14. Metabolism of benzo[a]pyrene and benzo[a]pyrene-7,8-diol by human cytochrome P450 1B1
15. Genetic epidemiology of environmental toxicity and cancer susceptibility: Human allelic polymorphisms in drug-metabolizing enzyme genes, their functional importance, and nomenclature issues
16. Baculovirus-mediated expression of cytochrome P4502C8 and human NADPH-cytochrome P450 reductase: Optimization of protein expression
17. Functional high level expression of cytochrome P4502D6 using baculoviral expression systems
18. In vitro kinetics of two human CYP1A1 variant enzymes suggested to be associated with interindividual differences in cancer susceptibility
19. Genetic polymorphism of drug-metabolizing enzymes and susceptibility to oral cancer
20. Allelic variants of human cytochrome P450 1A1 (CYP1A1): Effect of T461N and I462V substitutions on steroid hydroxylase specificity
21. Benzo[a]pyrene metabolites: Efficient and rapid separation by high-pressure liquid chromatography
22. Roles of individual human cytochrome P450 enzymes in the bioactivation of benzo[a]pyrene, 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene, and other dihydrodiol derivatives of polycyclic aromatic hydrocarbons
23. The role of 12 cDNA-expressed human, rodent, and rabbit cytochromes P450 in the metabolism of benzo[a]pyrene and benzo[a]pyrene trans-7,8-dihydrodiol
24. Baculovirus-mediated expression and functional characterization of human NADPH-P450 oxidoreductase
25. Competitive interactions between cytochromes P450 2A6 and 2E1 for NADPH-cytochrome P450 oxidoreductase in the microsomal membranes produced by a baculovirus expression system
26. Expression and coupling of human cytochrome P450 2E1 and NADPH-cytochrome P450 oxidoreductase in dual expression and co-infection systems with baculovirus in insect cells
27. Posttranslational regulation of cytochromes P4501A1 and 1A2
28. Some properties of a detergent-solubilized NADPH-cytochrome c (cytochrome P-450) reductase purified by biospecific affinity chromatography
29. 462: Assessment of a role for the rare allele in cancerogenesis
30. Relationship between cytochrome P450 catalytic cycling and stability: Fast degradation of ethanol-inducible cytochrome P450 2E1 (CYP2E1) in hepatoma cells is abolished by inactivation of its electron donor NADPH-cytochrome P450 reductase