Insertion-duplication mutagenesis of Neisseria: Use in characterization of DNA transfer genes in the gonococcal genetic island

Journal of Bacteriology

Volumn 183, Issue 16, 2001, Pages 4718-4726

  Hamilton, H L ^a   Schwartz, K J ^a   Dillard, J P ^a  

^a NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM TRANSFORMATION; CHROMOSOME MAP; DNA SEQUENCE; DNA TRANSFER; GENE DUPLICATION; GENE INSERTION; GENETIC RECOMBINATION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; MUTAGENESIS; NEISSERIA GONORRHOEAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION;

CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; CROSSING OVER, GENETIC; DNA, BACTERIAL; DRUG RESISTANCE, MICROBIAL; ERYTHROMYCIN; GENETIC VECTORS; METHYLTRANSFERASES; MODELS, GENETIC; MUTAGENESIS, INSERTIONAL; NEISSERIA GONORRHOEAE; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; RESTRICTION MAPPING; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA; NEISSERIA GONORRHOEAE;

EID: 0034903167     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.16.4718-4726.2001     Document Type: Article

References (46)

1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
2. Factors affecting genetic transformation of Neisseria gonorrhoeae
3. Shuttle mutagenesis: Two mini-transposons for gene mapping and for lacZ transcriptional fusions in Neisseria gonorrhoeae
4. Biochemistry of type IV secretion
5. Bacterial type IV secretion: Conjugation systems adapted to deliver effector molecules to host cells
6. A peptidoglycan hydrolase similar to bacteriophage endolysins acts as an autolysin in Neisseria gonorrhoeae
7. A variable genetic island specific for Neisseria gonorrhoeae is involved in providing DNA for natural transformation and is found more often in disseminated infection isolates
8. Genetic and molecular characterization of capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 3
9. Mechanism of integrating foreign DNA during transformation of Bacillus subtilis
10. Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence
11. Analysis of the sequence and gene products of the transfer region of the F sex factor
12. Genome plasticity in Neisseria gonorrhoeae
13. Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae
14. Use of a nonselective transformation technique to construct a multiply restriction/modification-deficient mutant of Neisseria gonorrhoeae
15. Rapid procedure for detection and isolation of large and small plasmids
16. Neisseria gonorrhoeae. Virulence genetically linked to clonal variation
17. Cloning of the recA gene of Neisseria gonorrhoeae and construction of gonococcal recA mutants
18. Enzymology of type IV macromolecule secretion systems: The conjugative transfer regions of plasmids RP4 and R388 and the cag pathogenicity island of Helicobacter pylori encode structurally and functionally related nucleoside triphosphate hydrolases
19. Insertion-duplication mutagenesis in Streptococcus pneumoniae: Targeting fragment length is a critical parameter in use as a random insertion tool
20. Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis
21. Single-crossover integration in the Lactobacillus sake chromosome and insertional inactivation of the ptsl and lacL genes
22. Selection of bacterial virulence genes that are specifically induced in host tissues
23. A homologue of the recombination-dependent growth gene, rdgC, is involved in gonococcal pilin antigenic variation
24. Intermolecular recombination during transformation of Bacillus subtilis competent cells by monomeric and dimeric plasmids
25. Isolation of transformation-deficient Streptococcus pneumoniae mutants defective in control of competence, using insertion-duplication mutagenesis with the erythromycin resistance determinant of pAMβ1
26. Factors affecting autolysis of Neisseria gonorrhoeae
27. Insertional mutagenesis in Bacillus subtilis: Mechanism and use in gene cloning
28. Genetic basis for colonial variation in Neisseria gonorrhoeae
29. Large-scale identification of virulence genes from Streptococcus pneumoniae
30. Insertional inactivation of the gene for the meningococcal lactoferrin binding protein
31. Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila
32. DNA transformation leads to pilin antigenic variation in Neisseria gonorrhoeae
33. Shuttle mutagenesis of Neisseria gonorrhoeae: Pilin null mutations lower DNA transformation competence
34. Shuttle mutagenesis: A method of transposon mutagenesis for Saccharomyces cerevisiae
35. Genetic mechanisms of bacterial antigenic variation
36. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance
37. Transformation of Neisseria gonorrhoeae: Physical requirements of the transforming DNA
38. Transposon-facilitated DNA sequencing
39. Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control
40. Identification of several rod loci and cloning of the rodD locus of Streptococcus mutans
41. Fluorescence-based isolation of bacterial genes expressed within host cells
42. Conjugative transfer by the virulence system of Legionella pneumophila
43. A conserved DNA sequence is required for efficient gonococcal pilin antigenic variation
44. The comP locus of Neisseria gonorrhoeae encodes a type IV prepilin that is dispensable for pilus biogenesis but essential for natural transformation
45. Plasmids with erythromycin resistance and catechol 2,3-dioxygenase- or beta-galactosidase-encoding gene cassettes for use in Neisseria spp.