Enzymology of type IV macromolecule secretion systems: The conjugative transfer regions of plasmids RP4 and R388 and the cag pathogenicity island of Helicobacter pylori encode structurally and functionally related nucleoside triphosphate hydrolases

Journal of Bacteriology

Volumn 182, Issue 10, 2000, Pages 2761-2770

  Krause, Sabine ^a   Pansegrau, Werner ^b,d   Lurz, Rudi ^a   De La Cruz, Fernando ^c   Lanka, Erich ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^b LEIDEN UNIVERSITY   (Netherlands)

^c UNIVERSITY OF CANTABRIA   (Spain)

^d CHIRON VACCINES   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROLASE; NUCLEOSIDE TRIPHOSPHATE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYMOLOGY; GENE MUTATION; HELICOBACTER PYLORI; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN TRANSPORT;

ACID ANHYDRIDE HYDROLASES; ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CONJUGATION, GENETIC; DNA, BACTERIAL; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HELICOBACTER PYLORI; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEOSIDE-TRIPHOSPHATASE; OLIGOPEPTIDES; PHOSPHOLIPIDS; PROTEIN CONFORMATION; R FACTORS; SOLUBILITY;

BACTERIA (MICROORGANISMS); EUKARYOTA; HELICOBACTER PYLORI; PROKARYOTA;

EID: 0034112006     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.10.2761-2770.2000     Document Type: Article

References (45)

1. Andersson, S. G. E., A. Zomorodipour, J. O. Andersson, T. Sicheritz-Pontén, U. C. M. Alsmark, R. M. Podowski, and C. G. Kurland. 1998. The genome sequence of Rickettsia prowazekii and the origin of mitochondria. Nature 396:133-140.
2. Balzer, D., W. Pansegrau, and E. Lanka. 1994. Essential motifs of relaxase (TraI) and TraG protein involved in conjugative transfer of plasmid RP4. J. Bacteriol. 176:4285-4295.
3. Balzer, D., G. Ziegelin, W. Pansegrau, V. Kruft, and E. Lanka. 1992. KorB protein of promiscuous plasmid RP4 recognizes inverted sequence repetitions in regions essential for conjugative plasmid transfer. Nucleic Acids Res. 20:1851-1858.
4. Binet, R., S. Letoffe, J. M. Ghigo, P. Delepelaire, and C. Wandersman. 1997. Protein secretion by Gram-negative bacterial ABC exporters - a review. Gene 192:7-11.
5. Bordo, D., and P. Argos. 1991. Suggestions for "safe" residue substitutions in site-directed mutageneses. J. Mol. Biol. 217:721-729.
6. Boyer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
7. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-154.
8. Burns, D. L. 1999. Biochemistry of type IV secretion. Curr. Opin. Microbiol. 2:25-29.
9. Censini, S., C. Lange, Z. Xiang, J. E. Crabtree, P. Ghiara, M. Borodovsky, R. Rappuoli, and A. Covacci. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factor. Proc. Natl. Acad. Sci. USA 93:14648-14653.
10. Christie, P. J., J. E. Ward, Jr., M. P. Gordon, and E. W. Nester. 1989. A gene required for transfer of T-DNA to plants encodes an ATPase with autophosphorylating activity. Proc. Natl. Acad. Sci. USA 86:9677-9681.
11. Covacci, A., J. L. Telford, G. D. Giudice, J. Parsonnet, and R. Rappuoli. 1999. Helicobacter pylori virulence and genetic geography. Science 284:1328-1333.
12. Crowther, R. A., and L. A. Amos. 1971. Harmonic analysis of electron microscope images with rotational symmetry. J. Mol. Biol. 60:123-130.
13. Daugelavičius, R., J. K. H. Bamford, A. M. Grahn, E. Lanka, and D. H. Bamford. 1997. IncP plasmid encoded cell envelope-associated DNA transfer complex increases the cell permeability. J. Bacteriol. 179:5195-5202.
14. de la Cruz, F., and E. Lanka. 1998. Function of the Ti-plasmid Vir proteins: T-complex formation and transfer to the plant cell, p. 281-301. In H. P. Spaink, A. Kondorosi, and P. J. J. Hooykaas (ed.), The Rhizobiaceae. Kluwer Academic Publishers, Boston, Mass.
15. Dowhan, W. 1997. CDP-diacylglycerol synthase of microorganisms. Annu. Rev. Biochem. 66:199-232.
16. Grahn, A. M., J. Haase, D. H. Bamford, and E. Lanka. 2000. Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and outer membranes of donor cells: implications for related macromolecule transport systems. J. Bacteriol. 182:1564-1574.
17. Haase, J., R. Lurz, A. M. Grahn, D. H. Bamford, and E. Lanka. 1995. Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex. J. Bacteriol. 177:4779-4791.
18. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
19. Hingorani, M. M., and M. O'Donnell. 1998. Toroidal proteins: running rings around DNA. Curr. Biol. 8:R83-R86.
20. Hueck, C. J. 1998. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol. Mol. Biol. Rev. 62:379-433.
21. Hwang, B. J., K. M. Woo, A. L. Goldberg, and C. H. Chung. 1988. Protease Ti, a new ATP-dependent protease in Escherichia coli, contains protein-activated ATPase and proteolytic functions in distinct subunits. J. Biol. Chem. 263:8727-8734.
22. Johnston, R. F., S. C. Pickett, and D. L. Barker. 1990. Autoradiography using storage phosphor technology. Electrophoresis 11:355-360.
23. Krause, S., M. Bárcena, W. Pansegrau, R. Lurz, J. M. Carazo, and E. Lanka. Sequence related protein export NTPases encoded by the conjugative transfer region of RP4 and by the cag pathogenicity island of Helicobacter pylori share similar hexameric ring structures. Proc. Natl. Acad. Sci. USA, in press.
24. Lakowicz, J. R. 1983. Principles of fluorescence spectroscopy. Plenum Press, New York, N.Y.
25. Lessl, M., D. Balzer, K. Weyrauch, and E. Lanka. 1993. The mating pair formation system of plasmid RP4 denned by RSF1010 mobilization and donor-specific phage propagation. J. Bacteriol. 175:6415-6425.
26. Lory, S. 1998. Secretion of proteins and assembly of bacterial surface organelles: shared pathways of extracellular protein targeting. Curr. Opin. Microbiol. 1:27-35.
27. Miller, J. H. 1972. Experiments in molecular genetics, p. 431-433. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Motallebi-Veshareh, M., D. Balzer, E. Lanka, G. Jagura-Burdzy, and C. M. Thomas. 1992. Conjugative transfer functions of broad-host-range plasmid RK2 are coregulated with vegetative replication. Mol. Microbiol. 6:907-920.
29. Pansegrau, W., and E. Lanka. 1996. Enzymology of DNA transfer by conjugative mechanisms. Prog. Nucleic Acid Res. Mol. Biol. 54:197-251.
30. Pansegrau, W., E. Lanka, P. T. Barth, D. H. Figurski, D. G. Guiney, D. Haas, D. R. Helinski, H. Schwab, V. A. Stanisich, and C. M. Thomas. 1994. Complete nucleotide sequence of Birmingham IncPα plasmids: compilation and comparative analysis. J. Mol. Biol. 239:623-663.
31. Possot, O., and A. P. Pugsley. 1994. Molecular characterization of PulE, a protein required for pullulanase secretion. Mol. Microbiol. 12:287-299.
32. Rashkova, S., G. M. Spudich, and P. J. Christie. 1998. Characterization of membrane and protein interaction determinants of the Agrobacterium tumefaciens VirB11 ATPase. J. Bacteriol. 179:583-591.
33. Rivas, S., S. Bolland, E. Cabezón, F. M. Goni, and F. de la Cruz. 1997. TrwD, a protein encoded by the IncW plasmid R388, displays an ATP hydrolyse activity essential for bacterial conjugation. J. Biol. Chem. 272:25583-25590.
34. Salmond, G. P. C. 1994. Secretion of extracellular virulence factors by plant pathogenetic bacteria. Annu. Rev. Phytopathol. 32:181-200.
35. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
36. Sandkvist, M., M. Bagdasarin, S. P. Howard, and V. J. DiRita. 1995. Interaction between the autokinase EspE and EspL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO J. 14:1664-1673.
37. Scherzinger, E., G. Ziegelin, M. Bárcena, J. M. Carazo, R. Lurz, and E. Lanka. 1997. The RepA protein of plasmid RSF1010 is a replicative DNA helicase. J. Biol. Chem. 272:30228-30236.
38. Segal, E. D., J. Cha, J. L. S. Falkow, and L. S. Tompkins. 1999. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc. Natl. Acad. Sci. USA 96:14559-14564.
39. Segal, G., M. Purcell, and H. A. Shuman. 1998. Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of genes of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA 95:1669-1674.
40. Stein, M., R. Rappuoli, and A. Covacci. 2000. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc. Natl. Acad. Sci. USA 97:1263-1268.
41. Stephens, K. M., C. Roush, and E. Nester. 1995. Agrobacterium tumefaciens VirB11 protein requires a consensus nucleotide-binding site for function in virulence. J. Bacteriol. 177:27-36.
42. Turner, L. R., J. W. Olson, and S. Lory. 1997. The XcpR protein of Pseudomonas aeruginosa dimerizes via its N-terminus. Mol. Microbiol. 26:877-887.
43. Vogel, P. J., H. L. Andrews, S. K. Wong, and R. R. Isberg. 1998. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279:873-875.
44. Winans, S. C., D. L. Burns, and P. J. Christie. 1996. Adaptation of a conjugal transfer system for the export of pathogenic macromolecules. Trends Microbiol. 4:64-68.
45. Zylicz, M., J. H. LeBowitz, R. McMacken, and C. Georgopoulos. 1983. The DnaK-protein of Escherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system. Proc. Natl. Acad. Sci. USA 80:6431-6435.