Application of proteomics to the study of cardiovascular biology

Trends in Cardiovascular Medicine

Volumn 11, Issue 2, 2001, Pages 66-75

  Macri, Joseph ^a   Rapundalo, Stephen T ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANAZOLENE SODIUM; FLUORESCENT DYE; PROTEOME; SILVER;

CARDIOVASCULAR DISEASE; DATA BASE; ELECTROPHORESIS; GENE EXPRESSION; HEART FAILURE; HEART INFARCTION; HUMAN; IMAGING; INFORMATION PROCESSING; INTERNET; NONHUMAN; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; REVIEW; SAMPLING; SIGNAL TRANSDUCTION; TECHNIQUE; TECHNOLOGY;

CARDIOVASCULAR DISEASES; FORECASTING; HUMANS; PROTEINS; PROTEOME; SEQUENCE ANALYSIS, PROTEIN;

EID: 0034890063     PISSN: 10501738     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1050-1738(01)00088-3     Document Type: Review

References (96)

1. A comparison of selected mRNA and protein abundances in human liver
2. Proteome and proteomics; new technologies, new concepts, and new words
3. An integrated approach to proteome analysis; identification of proteins associated with cardiac hypertrophy
4. A human myocardial two-dimensional electrophoresis database; protein characterisation by microsequencing and immunoblotting
5. Isoelectric focusing in immobilized pH gradients; principle, methodology and some applications
6. New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis
7. A comparison of three commercially available isoelectric focusing units for proteome analysis; the multiphor, the IPGphor and the protean IEF cell
8. The human myocardial two-dimensional gel protein database; update 1994
9. Coelectrophoresis of cardiac tissue from human, dog, rat and mouse. Towards the establishment of an integrated two-dimensional protein database
10. Cardiac protein abnormalities in dilated cardiomyopathy detected by two-dimensional polyacryamide gel electrophoresis
11. Identification of proteins by mass spectrometry
12. Two-dimensional gel electrophoresis of proteins
13. Construction of HSC-2DPAGE; a two-dimensional gel electrophoresis database of heart proteins
14. More on target with protein phosphorylation; conferring specificity by location
15. Advantages of immobilized pH gradients
16. Inappropriate ubiquitin conjugation; a proposed mechanism contributing to heart failure
17. Analysis of protein fractions by micropreparative capillary isoelectric focusing and matrix-assisted laser desorption time-of-flight mass spectrometry
18. Analysis of galectin 1-mediated cell signaling by combined precipitation and electrophoresis techniques
19. Protein identification methods in proteomics
20. Quantitation of human leukocyte proteins after silver staining; a study with two-dimensional electrophoresis
21. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt); the state of the art and the controversy of vertical versus horizontal systems
22. The current state of two-dimensional electrophoresis with immobilized pH gradients
23. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
24. One dimensional polyacrylamide gel electrophoresis
25. Biomedical applications of two-dimensional electrophoresis using immobilized pH gradients; current status
26. Proteomics; capacity versus utility
27. Glycosylation
28. Proteome analysis; biological assay or data archive?
29. Protein changes observed in pacing-induced heart failure using two-dimensional electrophoresis
30. Changes in myocardial protein expression in pacing-induced canine heart failure
31. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases
32. Advances in protein solubilisation for two-dimensional electrophoresis
33. Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent
34. Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining
35. Mapping cells and sub-cellular organelles on 2-D gels; 'new tricks for an old horse.'
36. Proteome research; complementarity and limitations with respect to the RNA and DNA worlds
37. Glycoproteins of rat skeletal muscle sarcolemma; characterization by two-dimensional gel electrophoresis and effect of denervation
38. Genome prospecting
39. Identification of human myocard proteins separated by two-dimensional electrophoresis
40. Protein composition of the human heart; the construction of a myocardial two-dimensional electrophoresis database
41. Enrichment of human brain proteins by heparin chromatography
42. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals
43. Genotypes and phenotypes
44. The major protein expression profile and two-dimensional protein database of human heart
45. Comparing 2-D electrophoretic gels across internet databases
46. A two-dimensional electrophoresis database of rat heart proteins
47. Improved two-dimensional gel electrophoresis representation of serum proteins by using ProtoClear
48. Advantages of carrier ampholyte IEF
49. Proteome analysis. I. Gene products are where the biologocal action is
50. Better approaches to finding the needle in a haystack; optimizing proteome analysis through automation
51. Characterization of the protein profile associated with endothelin-induced hypertrophy in neonatal rat myocytes
52. Cardiac sarcoplasmic reticulum and sarcolemmal proteins separated by two-dimensional electrophoresis; surfactant effects on membrane solubilization
53. Combination of electrophoretic techniques for comprehensive analysis of complex protein systems
54. Quantitative proteomics?
55. Human genome project; sequencers endorse plan for a draft in 1 year
56. Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level
57. Multisite phosphorylation and integration of stress signals at p53
58. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis
59. Two-dimensional electrophoresis with carrier ampholytes
60. High-performance human myocardial two-dimensional electrophoresis database; edition 1996
61. High resolution two-dimensional electrophoresis of proteins
62. Identification of human myocardial proteins separated by two-dimensional electrophoresis using an effective sample preparation for mass spectrometry
63. Proteomics; a major new technology for the drug discovery process
64. Protein profiling in cardiac tissue in response to the chronic effects of alcohol
65. Mass spectrometric approaches for the identification of gel-separated proteins
66. Proteome analysis. II. Protein subcellular redistribution; linking physiology to genomics via the proteome and separation technologies involved
67. A thousand points of light; the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics
68. The construction of the World-Wide Web-accessible myocardial two-dimensional gel electrophoresis protein database 'HEART-2DPAGE'; a pratical approach
69. Towards design and comparison of World Wide Web-accessible myocardial two-dimensional gel electrophoresis protein databases
70. Proteomics and automation
71. A comparison between low background silver diammine and silver nitrate protein stains
72. Solubilization of proteins for electrophoretic analyses
73. Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis
74. Solubilization of proteins in two-dimensional electrophoresis
75. Signal transduction pathways that regulate eukaryotic protein synthesis
76. A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms
77. Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients
78. Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels
79. Functional proteomics analysis of signal transduction pathways of the platelet-derived growth factor beta receptor
80. Applications of SYPRO orange and SYPRO red protein gel stains
81. Electronic imaging of electrophorectic gels and blots
82. Identification of myocardial proteins from two-dimensional gels by peptide mass fingerprinting
83. The analysis of myocardial proteins by infrared and ultraviolet laser desorption mass spectrometry
84. The genomic tree as revealed from whole proteome comparisons
85. Two-dimensional electrophoretic analysis of myocardial proteins from lead-exposed rabbits
86. Difference gel electrophoresis; a single gel method for detecting changes in protein extracts
87. Tantalizing transcriptomes-SAGE and its use in global gene expression analysis
88. The sequence of the human genome
89. Two-dimensional electrophoresis of cerebrospinal fluid and ventricular fluid proteins, identification of enriched and unique proteins, and comparison with serum
90. Bovine dilated cardiomyopathy; proteomic analysis of an animal model of human dilated cardiomyopathy
91. Progress with proteome projects; why all proteins expressed by a genome should be identified and how to do it
92. Introduction to the proteome
93. Mass spectrometry. From genomics to proteomics
94. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database