Identification of human myocardial proteins separated by two-dimensional electrophoresis using an effective sample preparation for mass spectrometry

Electrophoresis

Volumn 17, Issue 10, 1996, Pages 1643-1650

  Otto, Albrecht ^a   Thiede, Bernd ^a   Müller, Eva Christina ^a   Scheler, Christian ^c   Wittmann Liebold, Brigitte ^a,c   Jungblut, Peter ^a,b,c,d  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^b Inst F Transfusionsmedizin I   (Germany)

^c Wittmann Inst Technol Anal B   (Germany)

^d Proteinanalytik   (Germany)

Author keywords

Enzymatic in gel digestion; Matrix assisted laser desorption ionization mass spectrometry; Myocardial proteins; Peptide mass mapping; Two dimensional gel electrophoresis

Indexed keywords

BODY FLUIDS; ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PALMPRINT RECOGNITION; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

ENZYMATIC IN-GEL DIGESTION; INGEL DIGESTION; MASS MAPPING; MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; MYOCARDIAL PROTEIN; PEPTIDE MASS FINGERPRINTING; PEPTIDE MASS MAPPING; SAMPLE PREPARATION; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

PEPTIDES;

ACETYL COENZYME A ACYLTRANSFERASE; BOVINE SERUM ALBUMIN; CREATINE KINASE; CREATINE KINASE MB; CYTOCHROME C OXIDASE; FRUCTOSE BISPHOSPHATE ALDOLASE; ISOCITRATE DEHYDROGENASE; MYOGLOBIN; MYOSIN LIGHT CHAIN; PHOSPHOGLYCERATE KINASE;

ARTICLE; CONTROLLED STUDY; GEL ELECTROPHORESIS; HEART DILATATION; HEART MUSCLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; MASS SPECTROMETRY; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PURIFICATION; TECHNIQUE;

EID: 0029854465     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150171027     Document Type: Article

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