Galactosyl transfer catalyzed by thermostable β-glycosidases from Sulfolobus solfataricus and Pyrococcus furiosus: Kinetic studies of the reactions of galactosylated enzyme intermediates with a range of nucleophiles

Journal of Biochemistry

Volumn 130, Issue 3, 2001, Pages 341-349

  Petzelbauer, I ^a   Splechtna, B ^a   Nidetzky, B ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Binding; Kinetic partitioning; Nucleophile; Reactivity; Transgalactosylation

Indexed keywords

2 NITROPHENOL; 2 NITROPHENYL BETA DEXTRO GALACTOPYRANOSIDE; ALCOHOL DERIVATIVE; AZIDE; BETA GALACTOSIDE; BUTANOL; GALACTOSE; GLUCOSE; GLYCOSIDASE; HYDROXYL GROUP; LACTOSE(4 O BETA DEXTRO GALACTOPYRANOSYL DEXTRO GLUCOPYRANOSE); METHANOL; OCTANOL; PHENOL; PYRANOSIDE; UNCLASSIFIED DRUG; WATER;

ARTICLE; CATALYST; CHEMICAL REACTION KINETICS; ENZYME BINDING; ENZYME KINETICS; ENZYME SUBSTRATE; NONHUMAN; PROTEIN INTERACTION; PYROCOCCUS FURIOSUS; SULFOLOBUS SOLFATARICUS; SYNTHESIS; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PYROCOCCUS FURIOSUS; SULFOLOBUS SOLFATARICUS;

EID: 0034848317     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002992     Document Type: Article

References (38)

1. Thermostable β-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties
2. Purification and characterization of an extremely thermostable β-glucosidase from hyperthermophilic archaeon Pyrococcus furiosus
3. An extremely thermostable β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus; a comparison with other glycosidases
4. Expression and extensive characterization of a β-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus in Escherichia coli: Authenticity of the recombinant enzyme
5. Exo-glucosidase activity and substrate specificity of the β-glycosidase isolated from the extreme thermophile Sulfolobus solfataricus
6. Characterization of the celB gene coding for β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus and its expression and site-directed mutation in Escherichia coli
7. Glycosyl transfer
8. Mutagenesis of glycosidases
9. Structural and sequence-based classification of glycoside hydrolases
10. Identification of two glutamic acid residues essential for catalysis in the β-glycosidase from the thermoacidophilic archaeon Sulfolobus solfataricus
11. Identification of the active site nucleophile in the thermostable β-glycosidase from the archaeon Sulfolobus solfataricus expressed in Escherichia coli
12. β-Galactosidase
13. Enzymatic synthesis of polyol- and masked polyol-glycosides using β-glycosidase of Sulfolobus solfataricus
14. Catalytic potency of β-glucosidase from the extremophile Pyrococcus furiosus in glucoconjugate synthesis
15. A novel thermophilic glycosynthase that effects branching glycosylation
16. Restoration of the activity of active-site mutants of the hyperthermophilic β-glycosidase from Sulfolobus solfataricus: Dependence of the mechanism on the action of external nucleophiles
17. Structure-reactivity relationships for β-galactosidase (Escherichia coli, lac Z). 2. Reactions of the galactosyl-enzyme intermediate with alcohols and azide ion
18. Mechanism of Agrobacterium β-glycosidase: Kinetic analysis of the role of noncovalent enzyme/substrate interactions
19. Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) β-galactosidase
20. The family 1 β-glucosidases from Pyrococcus furiosus and Agrobacterium faecalis share a common catalytic mechanism
21. Design and synthesis of 2′-deoxy-2′-fluorodisaccharide as mechanism-based glycosidase inhibitors that exploit aglycon specificity
22. Crystal structure of the β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: Resilience as a key factor in thermostability
23. Comparative structural analysis and substrate specificity engineering of the hyperthermostable β-glucosidase CelB from Pyrococcus furiosus
24. Transgalactosylation by thermostable β-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus
25. Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. II. Oligosaccharide formation by two thermostable β-glycosidases
26. Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable β-glycosidases
27. β-Glucosidase CelB from Pyrococcus furiosus: Production by Escherichia coli, purification, and in vitro evolution
28. Conformational adaptability of the active site of β-galactosidase
29. Partition analysis and the concept of net rate constants as tools in enzyme kinetics
30. Nomenclature for sugar-binding subsites in glycosyl hydrolases
31. The effect of methanol and dioxan on the β-galactosidase-catalyzed hydrolyses of some β-D-galactopyranosides: Rate-limiting degalactosylation
32. Nucleophilic competition in some β-galactosidase-catalyzed reactions
33. Structure-reactivity relationships for β-galactosidase (Escherichia coli, lac Z). 4. Mechanism for reaction of nudeophiles with the galactosyl-enzyme intermediates of E461G and E461Q β-galactosidases
34. β-Galactosidase of Escherichia coli with substitutions for Glu-461 can be activated by nucleophiles and can form β-galactosyl adducts
35. β-Glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: Structure and activity in the presence of alcohols
36. Hydrophobic parameter: Measurement and calculation