Identification of two glutamic acid residues essential for catalysis in the β-glycosidase from the thermoacidophilic archaeon Sulfolobus solfataricus

Protein Engineering

Volumn 9, Issue 12, 1996, Pages 1191-1195

  Moracci, Marco ^a   Capalbo, Luisa ^a,b   Ciaramella, Maria ^a   Rossi, Mosé ^c  

^a Inst Protein Biochem Enzymol C   (Italy)

^b UNIVERSITY OF UTAH   (United States)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Chimeric enzymes; Glycosyl hydrolase active site; Site directed mutagenesis; Sulfolobus solfataricus; glucosidase

Indexed keywords

ARCHAEA; SCHISTOSOMA JAPONICUM; SULFOLOBUS SOLFATARICUS;

AMINO ACID; CHIMERIC PROTEIN; GLUTAMIC ACID; GLYCOSIDASE; MUTANT PROTEIN; THROMBIN; TRANSFERASE;

AMINO ACID SEQUENCE; ARCHAEBACTERIUM; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; HYDROLYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PURIFICATION; SCHISTOSOMA JAPONICUM; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY;

AMINO ACID SEQUENCE; BINDING SITES; ESCHERICHIA COLI; GLUCOSIDASES; GLUCOSIDES; GLUTAMIC ACID; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STEREOISOMERISM; SUBSTRATE SPECIFICITY; SULFOLOBUS;

EID: 0030459862     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.12.1191     Document Type: Article

References (24)

1. Barret, T., Suresh, C.G., Tolley, S.P., Dodson, E.J. and Hughes, M.A. (1995) Structure, 3, 951-960.
2. Bradford, M.M. (1976) Anal. Biochem., 72, 248-254.
3. Chen, L., Garret, T.P.J., Fincher, G.B. and Høj, P.B. (1995) J. Biol. Chem., 270, 8093-8101.
4. Davies, G. and Henrissat, B. (1995) Structure, 3, 853-859.
5. Davoodi, J., Wakarchuk, W.W., Campbell, R.L., Carey, P.R. and Surewicz, W.K. (1995) Eur. J. Biochem., 232, 839-843.
6. Henrissat, B (1991) Binchem. J., 280, 309-316.
7. Henrissat, B., Callebaut, I., Fabrega, S., Lehn, P., Mornon, J.P. and Davies, G. (1995) Proc. Natl Acad. Sci. USA, 92, 7090-7094.
8. Jenkins, J., Lo Leggio, L., Harris, G. and Pickersgill, R. (1995) FEBS Lett., 362, 281-285.
9. Kuipers, O.P., Boot, H.J. and de Vos, W.M. (1991) Nucleic Acids Res., 19, 4558.
10. Leatherbarrow, R.J. (1992) GraFit. Version 3.0, Erithacus Software Ltd, Staines, UK.
11. MacLeod, A.M., Lindhorst, T., Withers, S.G. and Warren, R.A.J. (1994) Biachemistry, 33, 6371-6376.
12. Mikaelian, I. and Sergeant, A. (1992) Nucleic Acids Res., 20, 376.
13. Moracci, M. Ciaramella, M., Nucci, R., Pearl, L.H., Sanderson, I., Trincone, A. and Rossi, M. (1994) Biocatalysis, 11, 89-103.
14. Moracci, M., Nucci, R., Febbraio, F., Vaccaro, C., Vespa, N., La Cara, F. and Rossi, M. (1995a) Enzyme Microbiol. Technol., 17, 992-997.
15. Moracci, M., Capalbo, L., De Rosa, M., La Montagna, R., Morana, A., Nucci, R., Ciaramella, M. and Rossi, M. (1995b) In Petersen, S.B., Svensson, B. and Pedersen, S. (eds), Carbohydrate Bioengineering. Elsevier Science BV, Amsterdam, The Netherlands, pp. 77-84.
16. Namchuk, M.N. and Withers, S.G. (1995) Biochemistry, 34, 16194-16202.
17. Nucci, R., Moracci, M., Vaccaro, C., Vespa, N. and Rossi, M. (1993) Biotechnol. Appl. Biochem., 17, 239-250.
18. Pearl, L.H., Hemmings, A.M., Nucci, R. and Rossi, M. (1993) J. Mol. Biol., 229, 558-560.
19. Sinnot, M.L. (1990) Chem. Rev., 90, 1171-1202.
20. Voorhost, W.G.B., Eggen, R.I.L., Luesink, E.J. and deVos, W.M. (1995) J. Bacteriol., 177, 7105-7111.
21. Wang, Q., Trimbur, D., Graham, R., Warren, R.A.J. and Withers, S.G. (1995) Biochemistry, 34, 14554-14562.
22. Wiesmann, C., Beste, G., Hengstenberg, W. and Schulz, G.E. (1995) Structure, 3, 961-968.
23. Withers, S.G., Warren, R.A.J., Street, I.P., Rupitz, K., Kempton, J.B. and Aebersold, R. (1990) J. Am. Chem. Soc., 112, 5887-5889.
24. Witt, E., Frank, R. and Hengstenberg, W. (1993) Protein Engng, 6, 913-920.