Synthesis of ligand-specific phage-display ScFv against the herbicide picloram by direct cloning from hyperimmunized mouse

Journal of Agricultural and Food Chemistry

Volumn 49, Issue 8, 2001, Pages 3628-3637

  Tout, N L ^a   Yau, K Y F ^a   Trevors, J T ^a   Lee, H ^a   Hall, J C ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

ELISA; Herbicides; Hyperimmunized mouse; Library; Panning; PCR; Phage display; Picloram; ScFv; Spleen

Indexed keywords

COAT PROTEIN; HERBICIDE; IMMUNOGLOBULIN; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN FV; OVALBUMIN; PICLORAM; RECOMBINANT ANTIBODY; SERUM ALBUMIN;

ANIMAL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIOPHAGE; CONTROLLED STUDY; DNA LIBRARY; DNA VECTOR; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE ISOLATION; GENE LIBRARY; GENETICS; IMMUNOGLOBULIN GENE; IMMUNOLOGY; LIGAND BINDING; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; MOUSE; MOUSE STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SOMATIC MUTATION; SPLEEN CELL; SYNTHESIS;

ANIMALS; BACTERIOPHAGES; BASE SEQUENCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE LIBRARY; GENETIC VECTORS; HERBICIDES; IMMUNOGLOBULIN FRAGMENTS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PICLORAM; POLYMERASE CHAIN REACTION;

ANIMALIA; BOVINAE;

EID: 0034838360     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf010136w     Document Type: Article

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