Hypoxia-inducible transgene expression in differentiated human NT2N neurons - A cell culture model for gene therapy of postischemic neuronal loss

Gene Therapy

Volumn 8, Issue 17, 2001, Pages 1357-1362

  Cao, Y J ^a,b   Shibata, T ^c   Rainov, N G ^d  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b BAYER PHARMA AG   (Germany)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Apoptosis; Hypoxia; Ischemia; Liposomes; Neuron

Indexed keywords

APOPTOSIS INHIBITOR; LIPOSOME; LUCIFERASE; NEUROTROPHIC FACTOR; PLASMID DNA; PLASMID VECTOR; PROTEIN BCL 2; RETINOIC ACID; VASCULOTROPIN;

APOPTOSIS; ARTICLE; BRAIN ISCHEMIA; CANCER CELL CULTURE; CELL ACTIVITY; CELL CULTURE; CELL LOSS; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA VECTOR; GENE EXPRESSION; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN TISSUE; HYPOXIA; MARKER GENE; MITOSIS; MODEL; NERVE CELL DIFFERENTIATION; NEUROPROTECTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; TRANSGENE;

APOPTOSIS; CELL LINE; ENDOTHELIAL GROWTH FACTORS; GENE EXPRESSION; GENE THERAPY; GENETIC VECTORS; HUMANS; ISCHEMIA; LUCIFERASES; LYMPHOKINES; NEURONS; PLASMIDS; PROTO-ONCOGENE PROTEINS C-BCL-2; TRANSGENES; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS;

CYTOMEGALOVIRUS;

EID: 0034836856     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301536     Document Type: Article

References (30)

1. Ischemic cell death in brain neurons
2. Apoptotic and antiapoptotic mechanisms in stroke
3. Exploiting the hypoxic cancer cell: Mechanisms and therapeutic strategies
4. Targeting gene expression to hypoxic tumor cells
5. Regulated expression of a foreign gene targeted to the ischaemic myocardium
6. Enhancement of gene expression under hypoxic conditions using fragments of the human vascular endothelial growth factor and the erythropoietin genes
7. An adenoviral vector regulated by hypoxia for the treatment of ischaemic disease and cancer
8. Characterization of physiologically regulated vectors for the treatment of ischaemic disease
9. Therapeutic efficacy of the suicide gene driven by the promoter of vascular endothelial growth factor gene against hypoxic tumor cell
10. Development of a hypoxia-responsive vector for tumor specific gene therapy
11. Pure, postmitotic, polarized human neurons derived from Ntera 2 cells provide a system for expressing exogenous proteins in termially differentiated neurons
12. Production of human CNS neurons from embryonal carcinoma cells using a cell aggregation method
13. Effect of barbiturates on hydroxyl radicals, lipid peroxidation, and hypoxic cell death in human NT2-N neurons
14. Cationic lipid-mediated transfection of cells in culture requires mitotic activity
15. Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum
16. Perspectives on oxygen sensing
17. Oxygen sensing and signaling: Impact on the regulation of physiologically important genes
18. Hypoxia-inducible expression of BAX: Application in tumor-targeted gene therapy
19. Adeno-associated virus vector-mediated bcl-2 gene transfer into post-ischemic gerbil brain in vivo: Prospects for gene therapy of ischemia-induced neuronal death
20. Herpes simplex viral vectors expressing Bcl-2 are neuroprotective when delivered after a stroke
21. Antiapoptotic Bcl-2 family protein expression increases with progression of oligodendroglioma
22. Up-regulation of Bcl-2 in microvascular endothelial cells enhances intratumoral angiogenesis and accelerates tumor growth
23. Lipid mediated gene delivery in the adult rat brain: Quantitative analysis of expression
24. Inefficient gene transfer by adenovirus vector to cystic fibrosis airway of mice and humans
25. Intratracheal gene delivery to the mouse airway: Characterisation of plasmid DNA expression and pharmacokinetics
26. Cationic liposome-mediated transfection: Effect of serum on expression and efficiency
27. Liposome-mediated gene transfer into normal and dystrophin-deficient mouse myoblasts
28. Condensation of plasmid DNA with polylysine improves liposome-mediated gene transfer into established and primary muscle cells
29. Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells
30. Interaction of cationic liposomes and their DNA complexes with monocytic leukemia cells