Purification of a model substrate for transcription factor phosphorylation by ERK2

Protein Expression and Purification

Volumn 23, Issue 1, 2001, Pages 191-197

  Waas, William F ^a   Dalby, Kevin N ^a,b  

^a Departments of Medicinal Chemistry   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; CELL GROWTH; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE TAGGING; HISTIDINE; MICHAELIS MENTEN KINETICS; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TEMPERATURE; TRANSCRIPTION FACTOR;

EID: 0034811241     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1491     Document Type: Article

References (28)

1. Protein-protein interactions in signaling cascades
2. Signaling from Ras to Rac and beyond: Not just a matter of GEFs
3. The causes of cancer: Implications for prevention and treatment
4. ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF
5. MAP kinase pathways
6. The Ras family of GTPases in cancer cell invasion
7. The Ras-RasGAP complex: Structural basis for GTPase activation and its loss in oncogenic Ras mutants
8. Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy
9. Studies on the structure and mechanism of H-ras p21
10. Targeting the Ras signaling pathway: A rational, mechanism-based treatment for hematologic malignancies?
11. Inhibition of ras oncogene: A novel approach to antineoplastic therapy
12. The role of Raf kinases in development and growth of tumors
13. raf oncogenes in carcinogenesis
14. PD98059 is an equipotent antagonist of the aryl hydrocarbon receptor and inhibitor of mitogen-activated protein kinase kinase
15. Purification of myelin basic protein from bovine brain
16. Catalytic reaction pathway for the mitogen-activated protein kinase ERK2
17. Conserved mechanisms of Ras regulation of evolutionary related transcription factors, Ets1 and Pointed P2
18. Ras-mediated phosphorylation of a conserved threonine residue enhances the transactivation activities of c-Ets1 and c-Ets2
19. Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site
20. Multiple docking sites on substrate proteins form a modular system that mediates recognition by ERK MAP kinase
21. Meaningful relationships: The regulation of the Ras/Raf/MEK/ERK pathway by protein interactions
22. Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases
23. Calculation of protein extinction coefficients from amino acid sequence data
24. Development of a method for analysis of free amino acids from physiological samples using a 420A ABI/PE amino acid analyzer
25. The kinetic mechanism of the dual phosphorylation of the ATF2 transcription factor by p38 mitogen-activated protein (MAP) kinase α
26. Synthetic hexapeptide substrates and inhibitors of 3′:5′-cyclic AMP-dependent protein kinase
27. Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK
28. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding