Properties of spin and fluorescent labels at a receptor-ligand interface

Biophysical Journal

Volumn 77, Issue 4, 1999, Pages 2237-2250

  Owenius, Rikard ^a   Österlund, Maria ^a   Lindgren, Mikael ^a   Svensson, Magdalena ^a   Olsen, Ole H ^b   Persson, Egon ^b   Freskgård, Per Ola ^b   Carlsson, Uno ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

^b NOVO NORDISK A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 7A; FLUORESCENT DYE; LIGAND; RECEPTOR; THROMBOPLASTIN;

ARTICLE; BLOOD CLOTTING; CIRCULAR DICHROISM; COVALENT BOND; ELECTRON SPIN RESONANCE; FLUORESCENCE ANALYSIS; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; MOLECULAR MODEL; PHYSICAL CHEMISTRY; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING; SIMULATION; SPIN LABELING;

EID: 0032850543     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)77064-5     Document Type: Article

References (35)

1. Banner, D. W., A. D'Arcy, C. Chène, F. K. Winkler, A. Guha, W. H. Konigsberg, Y. Nemerson, and D. Kirchhofer. 1996. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor. Nature. 380:41-46.
2. Brooks, B. R. III, R. E. Bruccoleri, B. D. Olafsen, D. J. States, S. Swaminthan, and M. Karplus. 1983. CHARMM: a program for macromolecular energy, minimization, and dynamics calculations. J. Comp. Chem. 4:187-217.
3. Cantor, C. R., and P. R. Schimmel. 1980. Biophysical Chemistry II: Techniques for the Study of Biological Structure and Function. W. H. Freeman and Company, San Francisco.
4. Connolly, M. L. 1983. Analytical molecular surface calculation. J. Appl. Crystallogr. 16:548-558.
5. Davie, E. W., K. Fujikawa, and W. Kisiel. 1991. The coagulation cascade: initiation, maintenance, and regulation. Biochemistry. 30:10363-10370.
6. 2+ and tissue factor studied using circular dichroism spectroscopy. Protein Sci. 5:1531-1540.
7. Gibbs, C. S., S. N. McCurdy, L. L. K. Leung, and L. R. Paborsky. 1994. Identification of the factor VIIa binding site on tissue factor by homologous loop swap and alanine scanning mutagenesis. Biochemistry. 33: 14003-14010.
8. Gill, S. C., and P. H. von. Hippel. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182:319-326.
9. Goldman, S. A., G. V. Bruno, and J. H. Freed. 1972. Estimating slow-motional rotational times for nitroxides by electron spin resonance. J. Phys. Chem. 76:1858-1860.
10. Hammarström, P., B. Kalman, B.-H. Jonsson, and U. Carlsson. 1998. Pyrene excimer fluorescence as a proximity probe for investigation of residual structure in the unfolded state of human carbonic anhydrase II. FEBS Lett. 420:63-68.
11. Harlos, K., D. M. A. Martin, D. P. O'Brien, E. Y. Jones, D. I. Stuart, I. Polikarpov, A. Miller, E. G. D. Tuddenham, and C. W. G. Boys. 1994. Crystal structure of the extracellular region of human tissue factor. Nature. 370:662-666.
12. Heiden, W., G. Moeckel, and J. Brickmann. 1993. A new approach to analysis and display of local lipophilicity/hydrophilicity mapped on molecular surfaces. J. Comp.-Aided Mol. Design. 7:503-514.
13. Hubbell, W. L., A. Gross, R. Langen, and M. A. Lietzow. 1998. Recent advances in site-directed spin labeling of proteins. Curr. Opin. Struct. Biol. 8:649-656.
14. Kelley, R. F., K. E. Costas, M. P. O'Connell, and R. A. Lazarus. 1995. Analysis of the factor VIIa binding site on human tissue factor: effects of tissue factor mutations on the kinetics and thermodynamics of binding. Biochemistry. 34:10383-10392.
15. Klug, C. S., S. S. Eaton, G. R. Eaton, and J. B. Feix. 1998. Ligand-induced conformational change in the ferric enterobactin receptor FepA as studied by site-directed spin labeling and time-domain ESR. Biochemistry. 37:9016-9023.
16. Likhtenshtein, G. I. 1993. Biophysical Labeling Methods in Molecular Biology. Cambridge University Press, New York.
17. Lindgren, M., M. Svensson, P.-O. Freskgård, U. Carlsson, B.-H. Jonsson, L.-G. Mårtensson, and P. Jonasson. 1993. Probing local mobility in carbonic anhydrase: EPR of spin-labelled SH groups introduced by site-directed mutagenesis. J. Chem. Soc., Perkin Trans. 2:2003-2008.
18. Lindgren, M., M. Svensson, P-O. Freskgård, U. Carlsson, P. Jonasson, L.-G. Mårtensson, and B.-H. Jonsson. 1995. Characterization of a folding intermediate of human carbonic anhydrase II: Probing local mobility by electron paramagnetic resonance. Biophys. J. 69:202-213.
19. Mann, K. G., M. E. Nesheim, W. R. Church, P. Haley, and S. Krishnaswamy. 1990. Surface-dependent reactions of the vitamin K-dependent enzyme complexes. Blood. 76:1-16.
20. Mchaourab, H. S., T. Kálai, K. Hideg, and W. L. Hubbell. 1999. Motion of spin-labeled side chains in T4 Lysozyme: effect of side chain structure. Biochemistry. 38:2947-2955.
21. Muller, Y. A., M. H. Ultsch, R. F. Kelley, and A. M. d. Vos. 1994. Structure of the extracellular domain of human tissue factor: location of the factor VIIa binding site. Biochemistry. 33:10864-10870.
22. Muller, Y. A., M. H. Ultsch, and A. M. d. Vos. 1996. The crystal structure of the extracellular domain of human tissue factor refined to 1.7 Å resolution. J. Mol. Biol. 256:144-159.
23. Nicholls, A., K. Sharp, and B. Honig. 1991. Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins: Struct., Fund., Genet. 11:281-296.
24. Persson, M., P. Hammarström, M. Lindgren, B.-H. Jonsson, M. Svensson, and U. Carlsson. 1999. EPR mapping of interactions between spin-labeled variants of human carbonic anhydrase II and GroEL: evidence for increased flexibility of the hydrophobic core by the interaction. Biochemistry. 38:432-441.
25. 2+ binding to the first epidermal growth factor-like domain of factor VIIa increases amidolytic activity and tissue factor affinity. J. Biol. Chem. 272:19919-19924.
26. Richardson, J. S. 1981. The anatomy and taxonomy of protein structure. Adv. Protein Chem. 34:167-339.
27. Riddles, P. W., R. L. Blakely, and B. Zemer. 1983. Reassessment of Ellman's reagent. Methods Enzymol. 91:49-60.
28. Ruf, W., C. R. Kelly, J. R. Schullek, D. M. A. Martin, I. Polikarpov, C. W. G. Boys, E. G. D. Tuddenham, and T. S. Edgington. 1995. Energetic contributions and topographical organization of ligand binding residues of tissue factor. Biochemistry. 34:6310-6315.
29. Ruf, W., J. R. Schullek, M. J. Stone, and T. S. Edgington. 1994. Mutational mapping of functional residues in tissue factor: identification of factor VII determinants in both structural modules of the predicted cytokine receptor homology domain. Biochemistry. 33:1565-1572.
30. Schneider, D. J., and J. H. Freed. 1989. Calculating slow motional magnetic resonance spectra. In Biological Magnetic Resonance, Vol. 8: Spin Labeling - Theory and Applications. L. J. Berliner and J. Reuben, editors. Plenum Press, New York. 1-76.
31. Schullek, J. R., W. Ruf, and T. S. Edgington. 1994. Key ligand interface residues in tissue factor contribute independently to factor VIIa binding. J. Biol. Chem. 269:19399-19403.
32. Stone, M. J., W. Ruf, D. J. Miles, T. S. Edgington, and P. E. Wright. 1995. Recombinant soluble human tissue factor secreted by Saccharomyces cerevisiae and refolded from Escherichia coli inclusion bodies: glycosylation of mutants, activity and physical characterization. Biochem. J. 310:605-614.
33. Svensson, M., P. Jonasson, P.-O. Freskgård, B.-H. Jonsson, M. Lindgren, L.-G. Mårtensson, M. Gentile, K. Borén, and U. Carlsson. 1995. Mapping the folding intermediate of human carbonic anhydrase II. Probing substructure by chemical reactivity and spin and fluorescence labeling of engineered cysteine residues. Biochemistry. 34:8606-8620.
34. Thim, L., S. Bjoern, M. Christensen, E. M. Nicolaisen, T. Lund-Hansen, A. H. Pedersen, and U. Hedner. 1988. Amino acid sequence and post-translational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells. Biochemistry. 27:7785-7793.
35. Zhang, E., R. St. Charles, and A. Tulinsky. 1999. Structure of extracellular tissue factor complexed with factor VIIa inhibited with a BPTI mutant. J. Mol. Biol. 285:2089-2104.