High-resolution probing of local conformational changes in proteins by the use of multiple labeling: Unfolding and self-assembly of human carbonic anhydrase II monitored by spin, fluorescent, and chemical reactivity probes

Biophysical Journal

Volumn 80, Issue 6, 2001, Pages 2867-2885

  Hammarström, Per ^a,b   Owenius, Rikard ^a   Mårtensson, Lars Göran ^a   Carlsson, Uno ^a   Lindgren, Mikael ^a,c  

^a LINKÖPING UNIVERSITY   (Sweden)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c SWEDISH DEFENCE RESEARCH AGENCY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

(1 OXYL 2,2,5,5 TETRAMETHYLPYRROLINE 3 METHYL)METHANETHIOSULFONATE; 5 [[[(2 IODOACETYL)AMINO]ETHYL]AMINO]NAPHTHALENE 1 SULFONIC ACID; 6 BROMOACETYL 2 DIMETHYLAMINONAPHTHALENE; CARBONATE DEHYDRATASE II; FLUORESCENT DYE; N (1 OXYL 2,2,5,5 TETRAMETHYL 3 PYRROLIDINYL)IODOACETAMIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANISOTROPY; ARTICLE; CHEMICAL LABELING; ENZYME DENATURATION; ENZYME STRUCTURE; MEASUREMENT; PHASE TRANSITION; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN FOLDING; SPIN LABELING; STRUCTURE ANALYSIS;

EID: 0035004520     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)76253-4     Document Type: Article

References (35)

1. Engineering the hydrophobic pocket of carbonic anhydrase II
2. Purification and properties of human erythrocyte carbonic anhydrases
3. Introduction of internal cysteines as conformational probes in yeast phosphoglycerate kinase
4. Probes for the conformational transitions of phosphorylase b. Effect of ligands studied by proton relaxation enhancement, fluorescence and chemical reactivities
5. Paramagnetic and fluorescent probes attached to "buried " sulfhydryl groups in human carbonic anhydrases. Application to inhibitor binding, denaturation and refolding
6. Analytical molecular surface calculation
7. Refined structure of human carbonic anhydrase II at 2.0 Å resolution
8. The GroES and GroEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures
9. Functional consequences of engineering the hydrophobic pocket of carbonic anhydrase II
10. Folding around the C-terminus of human carbonic anhydrase II. Kinetic characterization by use of a chemically reactive SH-group introduced by protein engineering
11. Assignment of the contribution of the tryptophan residues to the circular dichroism spectrum of human carbonic anhydrase II
12. Estimating slowmotional rotational times for nitroxides by electron spin resonance
13. Structure of native and apo carbonic anhydrase II and structure of some of its anion-ligand complexes
14. Pyrene excimer fluorescence as a proximity probe for investigation of residual structure in the unfolded state of human carbonic anhydrase II
15. Protein compactness measured by FRET: Human carbonic anhydrase II is considerably expanded by the interaction of GroEL
16. Structural mapping of an aggregation nucleation site in a molten-globule intermediate
17. A new approach to analysis and display of local lipophilicity/hydrophilicity mapped on molecular surfaces
18. Recent advances in site-directed spin labeling of proteins
19. Carbon-13 nuclear magnetic resonance probe of active-site ionizations in human carbonic anhydrase B
20. Diffusible, nonfibrillar ligands derived from A1-42are potent central nervous system neurotoxins
21. Probing local mobility in carbonic anhydrase. EPR of spin-labeled SH groups introduced by site-directed mutagenesis
22. Characterization of a folding intermediate of human carbonic anhydrase II: Probing local mobility by electron paramagnetic resonance
23. Determination of protein secondary structure and solvent accessibility using site-directed fluorescence labeling. Studies of T4 lysozyme using the fluorescent probe monobromobimane
24. Characterization of folding intermediates of human carbonic anhydrase II: Probing substructure by chemical labeling of SH groups introduced by site-directed mutagenesis
25. Contribution of individual tryptophan residues to the fluorescence spectrum of native and denatured forms of human carbonic anhydrase II
26. Electron spin labels
27. The preparation of guanidine hydrochloride
28. Amino acid composition of various forms of bovine and human erythrocyte carbonic anhydrase
29. Properties of spin and fluorescent labels at a receptor-ligand interface
30. The anatomy and taxonomy of protein structure
31. Calculating slow motional magnetic resonance spectra
32. Mapping the folding intermediate of human carbonic anhydrase II. Probing substructure by chemical reactivity and spin and fluorescence labeling of engineered cysteine residues