Mutations of the C-terminal end of cathepsin K affect proenzyme secretion and intracellular maturation

Biochemical and Biophysical Research Communications

Volumn 281, Issue 2, 2001, Pages 551-557

  Claveau, D ^a   Riendeau, D ^a  

^a MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Cathepsin K; Cysteine protease; Glycosylation; Intracellular activation; Targeting

Indexed keywords

BREFELDIN A; CATHEPSIN K; COMPLEMENTARY DNA; ENZYME PRECURSOR; MONENSIN; TUNICAMYCIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL ACTIVATION; CELL PH; CHO CELL; CONTROLLED STUDY; ENZYME GLYCOSYLATION; ENZYME RELEASE; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; IMMUNOFLUORESCENCE TEST; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING;

EID: 0034808706     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.4394     Document Type: Article

References (30)

1. Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts
2. Localization of cathepsin K in human osteoclasts by in situ hybridization and immunohistochemistry
3. Fluorescence microscopic demonstration of cathepsin K activity as the major lysosomal cysteine proteinase in osteoclasts
4. Study of immunoelectron microscopic localization of cathepsin K in osteoclasts and other bone cells in the mouse femur
5. Localization of rat cathepsin K in osteoclasts and resorption pits: Inhibition of bone resorption and cathepsin K-activity by peptidyl vinyl sulfones
6. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma
7. Expression of the elastolytic cathepsins S and K in human atheroma and regulation of their production in smooth muscle cells
8. Human cathepsin O2, a matrix protein-degrading cysteine protease expressed in osteoclasts. Functional expression of human cathepsin O2 in Spodoptera frugiperda and characterization of the enzyme
9. The collagenolytic activity of cathepsin K is unique among mammalian proteinases
10. Trafficking of matrix collagens through bone resorbing osteoclasts: A role for cathepsin K?
11. Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice
12. Mutations of CTSK result in pycnodysostosis via a reduction in cathepsin K protein
13. Characterization of novel cathepsin K mutations in the pro and mature polypeptide regions causing pycnodysostosis
14. Design of potent and selective human cathepsin K inhibitors that span the active site
15. Development and characterization of a human in vitro resorption assay: Demonstration of utility using novel antiresorptive agents
16. Structure-based development of pyridoxal propionate derivatives as specific inhibitors of cathepsin K in vitro and in vivo
17. Cathepsin K antisense oligodeoxynucleotide inhibits osteoclastic bone resorption
18. Cathepsin K knockout mice develop osteopetrosis due to a deficit in matrix degradation but not demineralization
19. Proteolytic activity of human osteoclast cathepsin K. Expression, purification, activation, and substrate identification
20. Autocatalytic activation of human cathepsin K
21. Expression, maturation and rhodamine based fluorescence assay of human cathepsin K expressed in CHO cells
22. Crystal structure of human cathepsin K complexed with a potent inhibitor
23. Processing and intracellular transport of cathepsin G and neutrophil elastase in the leukemic myeloid cell line U-937-modulation by brefeldin A, ammonium chloride, and monensin
24. Involvement of carboxy-terminal amino acids in secretion of human lysosomal protease cathepsin L
25. Effect of carbohydrate position on lysosomal transport of procathepsin L
26. Expression of functional recombinant human procathepsin B in mammalian cells
27. Sorting of non-glycosylated human procathepsin S in mammalian cells
28. Inhibitor studies indicate that active cathepsin L is probably essential to its own processing in cultured fibroblasts
29. Proteolytic processing and glycosylation of cathepsin B. The role of the primary structure of the latent precursor and of the carbohydrate moiety for cell-type-specific molecular forms of the enzyme
30. Molecular cloning of human cathepsin O, a novel endoproteinase and homologue of rabbit OC2