Fluorescence microscopic demonstration of cathepsin K activity as the major lysosomal cysteine proteinase in osteoclasts

Journal of Biochemistry

Volumn 123, Issue 4, 1998, Pages 752-759

  Kamiya, Takeshi ^a   Kobayashi, Yasuhiro ^a   Kanaoka, Kazuhiro ^a   Nakashima, Tomoki ^a   Kato, Yuzo ^a   Mizuno, Akio ^a   Sakai, Hideaki ^a  

^a NAGASAKI UNIVERSITY   (Japan)

Author keywords

Activity staining; Cathepsin K; Cysteine proteinase; Osteoclast

Indexed keywords

5 NITROSALICYLIC ACID; ALOXISTATIN; ANTIBODY; BAFILOMYCIN A1; CATHEPSIN; CHLOROQUINE; CYSTEINE PROTEINASE; CYSTEINE PROTEINASE INHIBITOR; LEUPEPTIN;

ANIMAL CELL; ARTICLE; BONE DEFECT; BONE MATRIX; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; FLUORESCENCE MICROSCOPY; HYDROLYSIS; IMMUNOADSORPTION; MOUSE; NEWBORN; NONHUMAN; OSTEOCLAST; PERITONEUM MACROPHAGE; PROTEIN DEGRADATION; RABBIT;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0031978083     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022001     Document Type: Article

References (40)

1. Teti, A., Marchisio, P.C., and Zambonin-Zallone, A. (1983) Clear zone in osteoclast function: role of podosomes in regulation of bone-resorbing activity. Am. J. Physiol. 261, C1-C7
2. Baron, R., Neff, L., Brown, W., Courtoy, P.J., Louvard, D., and Farquhar, M.G. (1988) Polarized secretion of lysosomal enzyme: co-distribution of cation-independent mannose-6-phosphate receptors and lysosomal enzymes along the osteoclast exocytic pathway. J. Cell Biol. 106, 1863-1872
3. Delaissé, J.M., Eeckhout, Y., and Vaes, G. (1980) Inhibition of bone resorption in culture by inhibitors of thiol proteinases. Biochem. J. 192, 365-368
4. Delaissé, J.M., Eeckhout, Y., and Vaes, G. (1984) In vivo and in vitro evidence for the involvement of cysteine proteinases in bone resorption. Biochem. Biophys. Res. Commun. 125, 441-447
5. Delaissé, J.M., Boyde, A., Maconnachie, E., Ali, N.N., Sear, C.H.J., Eeckhout, Y., and Vaes, G. (1987) The effects of inhibitors of cysteine-proteinases and collagenase on the resorptive activity of isolated osteoclasts. Bone 8, 305-313
6. Everts, V., Beertsen, W., and Schröder, R. (1988) Effects of the proteinase inhibitors leupeptin and E-64 on osteoclastic bone resorption. Calci. Tissue Int. 43, 172-178
7. Lerner, U.H. and Grubb, A. (1992) Human cystatin C, a cysteine proteinase inhibitor, inhibits bone resorption in vitro stimulated by parathyroid hormone and parathyroid hormone-related peptide of malignancy. J. Bone Miner. Res. 7, 433-440
8. Kakegawa, H., Nikawa, T., Tagami, K., Kamioka, H., Sumitani, K., Kawata, T., Drobnič-Kosorok, M., Lenarčič, B., Turk, V., and Katunuma, N. (1993) Participation of cathepsin L on bone resorption. FEBS Lett. 321, 247-250
9. Goto, T., Tsukuba, T., Kiyoshima, T., Nishimura, Y., Kato, K., Yamamoto, K., and Tanaka, T. (1993) Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast. Histochemistry 99, 411-414
10. Ohsawa, Y., Nitatori, T., Higuchi, S., Kominami, E., and Uchiyama, Y. (1993) Lysosomal cysteine and aspartic proteinases, acid phosphatase, and an endogenous cysteine proteinase inhibitor, cystatin-β, in rat osteoclasts. J. Histochem. Cytochem. 41, 1075-1083
11. Goto, T., Kiyoshima, T., Moroi, R., Tsukuba, T., Nishimura, Y., Himeno, M., Yamamoto, K., and Tanaka, T. (1994) Localization of cathepsins B, D, and L in the rat osteoclast by immuno-light and -electron microscopy. Histochemistry 101, 33-40
12. Tezuka, K., Tezuka, Y., Maejima, A., Sato, T., Nemoto, K., Kamioka, H., Hakeda, Y., and Kumegawa, M. (1994) Molecular cloning of a possible cysteine proteinase predominantly expressed in osteoclasts. J. Biol. Chem. 269, 1106-1109
13. Inaoka, T., Bilbe, G., Ishibashi, O., Tezuka, K., Kumegawa, M., and Kokubo, T. (1995) Molecular cloning of human cDNA for cathepsin K: novel cysteine proteinase predominantly expressed in bone. Biochem. Biophys. Res. Commun. 206, 89-96
14. Brömme, D. and Okamoto, K. (1995) Human cathepsin O2, a novel cysteine protease highly expressed in osteoclastomas and ovary molecular cloning, sequencing and tissue distribution. Biol. Chem. Hoppe-Seyler 376, 379-384
15. Li, Y-P., Alexander, M., Wucherpfenning, A.L., Yelick, P., Chen, W., and Stashenko, P. (1995) Cloning and complete coding sequence of a novel human cathepsin expressed in giant cells of osteoclastomas. J. Bone Miner. Res. 10, 1197-1202
16. Drake, F.H., Dodds, R.A., James, I.E., Connor, J.R., Debouck, C., Richardson, S., Lee-Rykaczewski, E., Coleman, L., Rieman, D., Barthlow, R., Hastings, G., and Gowen, M. (1996) Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts. J. Biol. Chem. 271, 12511-12516
17. Rantakokko, J., Aro, H.T., Savontaus, M., and Vuorio, E. (1996) Mouse cathepsin K: cDNA cloning and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development. FEBS Lett. 393, 307-313
18. Gelb, B.D., Moissoglu, K., Zhang, J., Martignetti, J.A., Brömme, D., and Desnick, R.J. (1996) Cathepsin K: isolation and characterization of the murine cDNA and genomic sequence, the homologue of the human pycnodysostosis gene. Biochem. Mol. Med. 59, 200-206
19. Bossard, M.J., Tomaszek, T.A., Thompson, S.K., Amegadzie, B.Y., Hanning, C.R., Jones, C., Kurdyla, J.T., McNulty, D.E., Darke, F.H., Gowen, M., and Levy, M.A. (1996) Proteolytic activity of human osteoclast cathepsin K. Expression, purification, activation, and substrate identification. J. Biol. Chem. 271, 12517-12524
20. Brömme, D., Okamoto, K., Wang, B.B., and Biroc, S. (1996) Human cathepsin O2, a matrix protein-degrading cysteine protease expressed in osteoclasts. Functional expression of human cathepsin O2 in Spodoptera frugiperda and characterization of the enzyme. J. Biol. Chem. 271, 2126-2132
21. Inui, T., Ishibashi, O., Inaoka, T., Inaoka, T., Origane, Y., Kumegawa, M., Kokubo, T., and Yamamura, T. (1997) Cathepsin K antisense oligodeoxynucleotide inhibits osteoclastic bone resorption. J. Biol. Chem. 272, 8109-8112
22. Littlewood, E.A., Kokubo, T., Ishibashi, O., Inaoka, T., Wlodarski, B., Gallagher, J.A., and Bilbe, G. (1997) Localization of cathepsin K in human osteoclasts by in situ hybridization and immunohistochemistry. Bone 20, 81-86
23. Tezuka, K., Sato, T., Kamioka, H., Nijweide, P.J., Tanaka, K., Matsuo, T., Ohta, M., Kurihara, N., Hakeda, Y., and Kumegawa, M. (1992) Identification of osteopontin in isolated rabbit osteoclasts. Biochem. Biophys. Res. Commun. 186, 911-917
24. Takahashi, N., Akatsu, T., Udagawa, N., Sasaki, T., Yamaguchi, A., Moseley, J.M., Martin, T.J., and Suda, T. (1988) Osteoblastic cells are involved in osteoclast formation. Endocrinology 123, 2600-2602
25. Hashimoto, F., Kobayashi, Y., Miyazaki, Y., Kamiya, T., Mataki, S., Kobayashi, K., Kato, Y., and Sakai, H. (1997) Antigenicity of pro-osteocalcin in hard tissue: the authenticity to visualize osteocalcin-producing cells. J. Bone Miner. Metab. 15, 122-131
26. Joseph, L.J., Chang, L.C., Stamenkovich, D., and Sukhatme, V.P. (1988) Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts. J. Clin. Invest. 81, 1621-1629
27. Yoshimori, T., Semba, T., Takemoto, H., Akagi, S., Yamamoto, A., and Tashiro, Y. (1990) Protein disulfide-isomerase in rat exocrine pancreatic cells is exported from the endoplasmic reticulum despite possessing the retention signal. J. Biol. Chem. 265, 15984-15990
28. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275
29. Väänänen, H.K. and Horton, M. (1995) The osteoclast clear zone is a specialized cell-extracellular matrix adhesion structure. J. Cell Sci. 108, 2729-2732
30. Dolbeare, F.A. and Smith, R.E. (1977) Flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde and 4-methoxy-β-naphthylamine derivatives. Clin. Chem. 23, 1485-1491
31. Graf, M., Leemann, U., Ruch, F., and Sträuli, P. (1979) The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts. Histochemistry 64, 319-322
32. Dolbeare, F. and Vanderlaan, M. (1979) A fluorescent assay of proteinases in cultured mammalian cells. J. Histochem. Cytochem. 27, 1493-1495
33. van Noorden, C.J.F., Vogels, I.M.C., Everts, V., and Beertsen, W. (1987) Localization of cathepsin B activity in fibroblasts and chondrocytes by continuous monitoring of the formation of a final fluorescent reaction product using 5-nitrosalicylaldehyde. Histochem. J. 19, 483-487
34. Sakai, K., Nii, Y., Ueyama, A., and Kishino, Y. (1991) Fluorescence demonstration of cathepsin B activity in fractionated alveolar macrophages. Cell. Mol. Biol. 37, 353-358
35. Reddy, V.Y., Zhang, Q-Y., and Weiss, S.J. (1995) Pericellular mobilization of the tissue-destructive cysteine proteinases, cathepsins B, L, and S, by human monocyte-derived macrophages. Proc. Natl. Acad. Sci. USA 92, 3849-3853
36. Kayser, L., Perrild, H., Thomsen, J., and Høyer, P.E. (1996) Microfluorometric kinetic analysis of cathepsin B activity in single human thyroid follicular epithelial cells using image analysis and continuous monitoring. Histochem. J. 28, 257-263
37. Jimi, E., Nakamura, I., Amano, H., Taguchi, Y., Tsurukai, T., Tamura, M., Takahashi, N., and Suda, T. (1996) Osteoclast function is activated by osteoblastic cells through a mechanism involving cell-to-cell contact. Endocrinology 137, 2187-2190
38. +-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells. J. Biol. Chem. 266, 17707-17712
39. Aibe, K., Yazawa, H., Abe, K., Teramura, K., Kumegawa, M., Kawashima, H., and Honda, K. (1996) Substrate specificity of recombinant osteoclast-specific cathepsin K from rabbits. Biol. Pharm. Bull. 19, 1026-1031
40. Kakudo, S., Miyazawa, K., Kameda, T., Mano, H., Mori, Y., Yuasa, T., Nakamaru, Y., Shiokawa, M., Nagahira, K., Tokunaga, S., Hakeda, Y., and Kumegawa, M. (1996) Isolation of highly enriched rabbit osteoclasts from collagen gels: A new assay system for bone-resorbing activity of mature osteoclasts. J. Bone Miner. Metab. 14, 129-136