Development and characterization of a human in vitro resorption assay: Demonstration of utility using novel antiresorptive agents

Journal of Bone and Mineral Research

Volumn 14, Issue 9, 1999, Pages 1562-1569

  James, Ian E ^a   Lark, Michael W ^a   Zembryki, Denise ^a   Lee Rykaczewski, Elizabeth V ^a   Hwang, Shing Mei ^a   Tomaszek, Thaddeus A ^a   Belfiore, Pietro ^b   Gowen, Maxine ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b SmithKline Beecham SpA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE INHIBITOR; CATHEPSIN K; COLLAGEN TYPE 1; LIQUID NITROGEN; PROCOLLAGEN C PROTEINASE; PROCOLLAGEN N PROTEINASE; PROTEIN INHIBITOR; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; SB 240314; SB 268338; SB 270022; SB 271448; UNCLASSIFIED DRUG; VITRONECTIN RECEPTOR;

ANIMAL CELL; ARTICLE; CATTLE; CELL ADHESION; CONTROLLED STUDY; CORTICAL BONE; CRYOPRESERVATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; NONHUMAN; OSTEOCLAST; OSTEOLYSIS;

ADENOSINE TRIPHOSPHATASES; ANIMALS; BONE NEOPLASMS; BONE RESORPTION; CATHEPSINS; CATTLE; CELL ADHESION; CELL SEPARATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; FREEZING; GIANT CELL TUMOR OF BONE; HUMANS; PHENOTYPE; REPRODUCIBILITY OF RESULTS; TUMOR MARKERS, BIOLOGICAL;

EID: 0032831651     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/jbmr.1999.14.9.1562     Document Type: Article

References (39)

1. Walker DT 1973 Osteoporosis cured by temporary parabiosis. Science 180:275.
2. Kahn AJ, Simmons DJ 1975 Investigation of cell lineage in bone using a chimaera of chick and quail embryonic tissue. Nature 258:325-327.
3. Murrills RJ, Dempster DW, Amett TR 1998 Isolation and culture of osteoclasts and osteoclast resorption assays. In: Arnett TR, Henderson B (eds.) Methods in Bone Biology, 1st Ed. Chapman & Hall, Ltd., London, U.K, pp. 64-105.
4. Collin-Osdoby P, Oursler MJ, Webber D, Osdoby P 1991 Osteoclast-specific monoclonal antibodies coupled to magnetic beads provide a rapid and efficient method of purifying avian osteoclasts. J Bone Miner Res 6:1353-1365.
5. Flanagan AM, Sarma U 1998 In vitro models for osteoclast recruitment. In: Amett TR, Henderson B (eds.) Methods in Bone Biology, 1st Ed. Chapman & Hall, Ltd., London, U.K, pp. 64-105.
6. Yoneda T, Alsina MM, Garcia JL, Mundy GR 1991 Differentiation of HL-60 cells into cells with the osteoclast phenotype. Endocrinology 9:683-689.
7. Gattei V, Bernabei PA, Pinto A, Bezzini R, Ringressi A, Formigli L, Tanini A, Attadia V, Brandi ML 1992 Phorbol ester induced osteoclast-like differentiation of a novel human leukemic cell line (FLG 29.1). J Cell Biol 116:437-447.
8. Grano M, Colucci S, De Bellis M, Zigrino P, Argentino L, Zambonin G, Serra M, Scotlandi K, Teti A, Zambonin Zallone A 1994 New model for bone resorption study in vitro: Human osteoclast-like cells from giant cell tumors of bone. J Bone Miner Res 9:1013-1020.
9. Takada Y, Kusuda M, Hiura K, Sato T, Mochizuki H, Nagao Y, Tomura M, Yahiro M, Hakeda Y, Kawashima H, Kumegawa M 1992 A simple method to assess osteoclast-mediated bone resorption using unfractionated bone cells. Bone Miner 17:347-359.
10. Hata K, Kukita T, Akamine A, Kukita A, Kurisu K 1992 Trypsinized osteoclast-like cells formed in rat bone marrow cultures efficiently form resorption lacunae on dentine. Bone 13:139-146.
11. Chambers TJ, Revell PA, Fuller K, Athanasou NA 1984 Resorption of bone by isolated rabbit osteoclasts. J Cell Sci 66:383-399.
12. Zambonin-Zallone A, Teti A, Primavera MV 1982 Isolated osteoclasts in primary culture: First observations on structure and survival in culture media. Anat Embryol 165:405-413.
13. James IE, Walsh S, Dodds RA, Gowen M 1991 Production ans characterization of osteoclast-selective monoclonal antibodies that distinguish between multinucleated cells derived from different human tissues. J Histochem Cytochem 39:905-914.
14. James IE, Dodds RA, Lee-Rykaczewski E, Eichman CF, Connor JR, Hart TK, Maleeff BE, Lackman RD, Gowen M 1996 Purification and characterization of fully functional human osteoclast precursors. J Bone Miner Res 11:1608-1618.
15. Blair HC, Teitelbaum SL, Ghiselli R, Gluck S 1989 Osteoclastic bone resorption by a polarized vacuolar proton pump. Science 245:855-857.
16. Goldring SR, Roelke MS, Petrison KK, Bhan AK 1987 Human giant cell tumors of bone: Identification and characterization of cell types. J Cell Invest 79:483-491.
17. Chambers TJ, Fuller K, McSheehy PMJ 1985 The effects of calcium regulating hormones on bone resorption by isolated human osteoclastoma cells. J Pathol 145:297-305.
18. Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M 1989 The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitro-nectin receptor. J Cell Biol 109:1817-1826.
19. Drake FH, Dodds RA, James IE, Connor JR, Debouck C, Richardson S, Lee-Rykaczewski E, Colman L, Rieman D, Barthlow R, Hastings G, Gowen M 1996 Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts. J Biol Chem 271:12511-12516.
20. Foged NT, Delaisse J-M, Hou P, Lou H, Sato T, Winding B, Bonde M 1996 Quantification of the collagenolytic activity of isolated osteoclasts by Enzyme-linked immunosorbent assay. J Bone Miner Res 11:226-237.
21. Apone S, Lee M, Eyre D 1997 Osteoclasts generate cross-linked collagen N-telopeptides (NTx) but not free-pyrodinolines when cultured on human bone. Bone 21:129-136.
22. Lark MW, Miller WH, Stroup GB, Hwang SM, James IE, Drake FH, Bradbeer J, Bondinell WE, Cousins RD, Erhard KF, Haltiwanger RC, Jakas DR, Keenan RM, Ku TW, Mathur A, Newlander KA, Ross ST, Adams PC, Azzarano LM, Smith BR, Huffman WH, Gowen M 1999 Design and characterization of an orally active Arg-Gly-Asp peptidomimetic vitronectin receptor antagonist, SB 265123, for the prevention of bone loss in osteoporosis. J Pharm Exp Therap (in press).
23. +-ATPase of osteoclasts. J Med Chem 41:1568-1573.
24. Thompson SK, Halbert SM, Bossard MJ, Tomaszek TA, Levy MA, Zhaou B, Smith WW, Abdel-Meguid SS, Janson CA, D'Alessio KJ, McQueney MS, Amegadzie BY, Hanning CR, DesJarlais RL, Briand J, Sarkar SK, Huddleston MJ, Ijames CF, Carr SA, Garnes KT, Shu A, Heys JR, Bradbeer J, Zembryki D, Lee-Rykaczewski L, James IE, Lark MW, Drake FH, Gowen M, Gleason JG, Veber DF 1997 Design of potent and selective human cathepsin K inhibitors which span the active site. Proc Natl Acad Sci USA 94:14249-14254.
25. Yamashita DS, Smith WW, Zhao B, Janson CA, Tomaszek TA, Bossard MJ, Levy MA, Oh H-J, Thompson SK, Ijames CF, Carr SA, McQueney M, D'Alessio KJ, Amegadzie BY, Hanning CR, Abdel-Meguid S, DesJarlais RL, Gleason JG, Veber DF 1997 Structure and design of potent and selective cathepsin K inhibitors. J Am Chem Soc 119:11351-11352.
26. Boyde A, Jones SJ 1984 Resorption of dentine by isolated osteoclasts in vitro. Br Dent J 156:216-220.
27. Jones SJ, Boyde A, Piper K 1992 Confocal microscopic mapping of osteoclastic resorption. Microscopy Anal 7:18-20.
28. Kumar CS, James IE, Wong A, Mwangi V, Field JA, Nuthulaganti P, Connor JR, Eichman C, Ali F, Hwang S-M, Rieman DJ, Drake FH, Gowen M 1996 Cloning and characterization of a novel integrin β3 subunit. J Biol Chem 272:16390-16397.
29. Väänänen HK, Karhukorpi E-K, Sundquist K, Wallmark B, Roininen I, Hentunen T, Tuukkanen J, Lakkakorpi P 1990 Evidence for the presence of a proton pump of the vavuolar H+ ATPase type in the ruffled borders of osteoclasts. J Cell Biol 111:1305-1311.
30. 2+-stimulated ATPase activity. Anal Biochem 157:375-380.
31. Votta BJ, Levy MA, Badger A, Bradbeer J, Dodds RA, James IE, Thompson S, Bossard MJ, Carr T, Connor JR, Tomaszek TA, Szewczuk L, Drake FH, Veber DF, Gowen M 1997 Peptide aldehyde inhibitors of cathepsin K inhibit bone resorption both in vitro and in vivo. J Bone Miner Res 12:1396-1406.
32. 3 potentiate bone resorption. J Biol Chem 268:9901-9907.
33. Sato M, Sardana MK, Grasser WA, Garsky VM, Murray JM, Gould RJ 1990 Echistatin is a potent inhibitor of bone resorption in culture. J Cell Biol 111:1713-1723.
34. 3) antagonists. J Med Chem 40:2289-2292.
35. Breuil V, Cosman F, Stein L, Horbert W, Nieves J, Shen V, Lindsay R, Dempster DW 1998 Human osteoclast formation and activity in vitro: Effects of alendronate. J Bone Miner Res 13:1721-1729.
36. Boyde A, Jones SJ 1991 Pitfalls in pit measurement. Calcif Tissue Int 49:65-70.
37. James IE, Zembryki D, Lee-Rykaczewski E, Foged NT, Keenan R, Miller WH, Hwang S-M, Gowen M 1996 Nonpeptide vitronectin receptor antagonists inhibit bone resorption by human osteoclasts. J Bone Miner Res 11:S288.
38. Atley LM, Mort JS, Lalumiere M, Eyre DR 1997 Quantitative release by cathepsin K of immunoreactive cross-linked N-telopeptides from bone type I collagen. J Bone Miner Res 12:S417.
39. Akisaka T, Gay CV 1986 Ultracytochemical evidence for a proton-pump adenosine triphosphate in chick osteoclasts. Cell Tissue Res 245:507-512.