Stable expression of human 5α-reductase type II in COS1 cells due to chromosomal gene integration: A novel tool for inhibitor identification

Journal of Steroid Biochemistry and Molecular Biology

Volumn 78, Issue 3, 2001, Pages 275-284

  Reichert, Wolfgang ^a   Michel, Armin ^a   Hartmann, Rolf W ^a   Jose, Joachim ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

5 reductase; COS1; Hyperplasia; Plasmid; Stable expression

Indexed keywords

17BETA (N ADAMANTOYLCARBAMOYL)ANDROST 4 ENE 3,6 DIONE; 20 HYDROXYIMINOPREGN 4 EN 3 ONE; 21 HYDROXYIMINO 21 METHYLPREGN 4 EN 3,6 DIONE; 21 HYDROXYIMINOPREGN 4 EN 3 ONE; 6 [3 (N,N DICYCLOHEXYLCARBAMOYL)PHENYL] 3,4 DIHYDRONAPHTHALENE 2 CARBOXYLIC ACID; ANTIBIOTIC G 418; CARBOXYLIC ACID DERIVATIVE; EB 33; ENZYME INHIBITOR; FP 12; FP 7; ISOENZYME; MH 49; MH 54; MH 62; MH 65; N (DICYCLOHEXYL)ACETYLPIPERIDINE 4 (BENZYL 4 CARBOXYLIC ACID); N (DICYCLOHEXYL)ACETYLPIPERIDINE 4 (BENZYLIDENE 4 CARBOXYLIC ACID); STEROID; STEROID REDUCTASE; SULFATE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; ASSAY; CELL ACTIVITY; CELL LINE; CHROMOSOME STRUCTURE; CONTROLLED STUDY; DNA VECTOR; DRUG EFFECT; DRUG SCREENING; ENZYME ACTIVITY; FLUORESCENCE IN SITU HYBRIDIZATION; IC 50; MOLECULAR CLONING; MOLECULAR STABILITY; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; SOUTHERN BLOTTING;

ANDROSTENEDIONE; ANIMALS; BASE SEQUENCE; CHOLESTENONE 5 ALPHA-REDUCTASE; CHROMOSOME MAPPING; COS CELLS; DIHYDROTESTOSTERONE; DNA PRIMERS; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; GENE EXPRESSION; HUMANS; ISOENZYMES; MALE; OXIDOREDUCTASES; PLASMIDS; PROSTATIC HYPERPLASIA; RECOMBINANT PROTEINS; TRANSFECTION;

CERCOPITHECUS AETHIOPS;

EID: 0034797880     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-0760(01)00092-9     Document Type: Article

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