Xis protein of the conjugative transposon Tn916 plays dual opposing roles in transposon excision

Molecular Microbiology

Volumn 41, Issue 6, 2001, Pages 1459-1467

  Hinerfeld, D ^a   Churchward, G ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; GUANINE; NUCLEASE; PROTEIN; UNCLASSIFIED DRUG; XIS PROTEIN;

ARTICLE; BACTERIUM CONJUGATION; BINDING AFFINITY; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; EXCISION; GEL MOBILITY SHIFT ASSAY; GENE MUTATION; METHYLATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; TRANSPOSON;

BASE SEQUENCE; BINDING SITES; CONJUGATION, GENETIC; DNA NUCLEOTIDYLTRANSFERASES; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; MUTATION; STAPHYLOCOCCUS PHAGES; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); PROKARYOTA;

EID: 0034786513     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02626.x     Document Type: Article

References (43)

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3. Conjugative transposition of Tn916: The transposon int gene is required only in the donor
4. Heterogeneity of tetracycline resistance determinants in Streptococcus
5. Control of directionality in lambda site-specific recombination
6. Determinants of directionality in lambda site-specific recombination
7. Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism
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12. Site-specific DNA binding using a variation of the double stranded RNA binding motif
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14. Conjugative transfer of Tn916 in Enterococcus faecalis: Trans activation of homologous transposons
15. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of conjugative transfer in the absence of a conjugative plasmid
16. A transposon in Streptococcus faecalis with fertility properties
17. The lambda phage att site: Functional limits and interaction with Int protein
18. A functional origin of transfer (oriT) on the conjugative transposon Tn916
19. Interactions of the integrase protein of the conjugative transposon Tn916 with its specific DNA binding sites
20. Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR
21. Mapping of a higher order protein-DNA complex: Two kinds of long range interaction in lambda attL
22. Lambda Xis degradation invivoby Lon and FtsH
23. Conjugative transposition: Tn916 integrase contains two independent DNA binding domains that recognize different DNA sequences
24. Regulation of excision of the conjugative transposon Tn916
25. The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision
26. A switch in the formation of alternative DNA loops modulates lambda site-specific recombination
27. Autonomous DNA binding domains of lambda integrase recognize two different sequence families
28. Suicide recombination substrates yield covalent lambda integrase-DNA complexes and lead to identification of the active site tyrosine
29. Step-arrest mutants of Flp recombinase: Implications for the catalytic mechanism of DNA recombination
30. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: Homologies with other site-specific recombinases
31. Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916
32. DNA binding by the Xis protein of the conjugative transposon Tn916
33. Conjugative transposition
34. An intermediate in the transposition of the conjugative transposon Tn916
35. Conjugative transposition of Tn916: Preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate
36. Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase
37. Characterization of the left 4 kb of conjugative transposon Tn916: Determinants involved in excision
38. Specific DNA cleavage mediated by the Integrase of conjugative transposon Tn916
39. Empirical estimation of protein-induced DNA bending angles: Applications to lambda site-specific recombination complexes
40. Empirical estimation of protein-induced DNA bending angles: Applications to λ site-specific recombination complexes
41. Protein-protein interactions in a higher-order structure direct lambda site-specific recombination
42. Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector
43. Interaction of the lambda site-specific recombination protein Xis with attachment site DNA