The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision

Journal of Bacteriology

Volumn 181, Issue 17, 1999, Pages 5414-5418

  Marra, Diana ^a,b   Pethel, Beth ^b   Churchward, Gordon G ^b   Scott, June R ^b  

^a UNIVERSITY OF COLORADO   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; EXCISIONASE; INTEGRASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM CONJUGATION; CONCENTRATION RESPONSE; DNA CONFORMATION; DNA DAMAGE; DNA TRANSPOSITION; ENTEROCOCCUS FAECALIS; GENE EXPRESSION; GENE OVEREXPRESSION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; TRANSPOSON;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; POSIBACTERIA; PROKARYOTA;

EID: 0032881672     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.17.5414-5418.1999     Document Type: Article

References (25)

1. Bringel, F., G. L. Van Alstine, and J. R. Scott. 1991. A host factor absent from Lactococcus lactis subspecies lactis MG1363 is required for conjugative transposition. Mol. Microbiol. 5:2983-2993.
2. Bringel, F. G., G. L. Van Alstine, and J. R. Scott 1992. Conjugative transposition of Tn916: the transposon int gene is required only in the donor. J. Bacteriol. 174:4036-4041.
3. Caparon, M. G., and J. R. Scott. 1989. Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism. Cell 59:1027-1034.
4. Celli, J., and P. Trieu-Cuot. 1998. Circularization of Tn916 is required for expression of the transposon-encoded transfer functions: characterization of long tetracycline-inducible transcripts reading through the attachment site. Mol. Microbiol. 28:103-117.
5. Chang, A. C., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
6. Clewell, D. B., S. E. Flannagan, and D. D. Jaworski. 1995. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol. 3:229-236.
7. Evans, R. P. J., and F. L. Macrina. 1983. Streptococcal R plasmid pIP501: endonuclease site map, resistance determinant location, and construction of novel derivatives. J. Bacteriol. 154:1347-1355.
8. Flannagan, S. E., L. A. Zitzow, Y. A. Su, and D. B. Clewell. 1994. Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis. Plasmid 32:350-354.
9. Franke, A. E., and D. B. Clewell. 1981. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of conjugative transfer in the absence of a conjugative plasmid. J. Bacteriol. 145:494-502.
10. Horaud, T., G. de Cespedes, D. Clermont, F. David, and F. Delbos. 1991. Variability of chromosomal genetic elements in streptococci, p. 16-20. In G. M. Dunny, P. P. Cleary, and L. L. McKay (ed.), Genetics and molecular biology of streptococci, lactococci, and enterococci. American Society for Microbiology, Washington, D.C.
11. Jaworski, D. D., and D. B. Clewell. 1994. Evidence that coupling sequences play a frequency-determining role in conjugative transposition of Tn916 in Enterococcus faecalis. J. Bacteriol. 176:3328-3335.
12. Jaworski, D. D., and D. B. Clewell. 1995. A functional origin of transfer (oriT) on the conjugative transposon Tn916. J. Bacteriol. 177:6644-6651.
13. Leffers, G. G., and S. Gottesman. 1998. Lambda Xis degradation in vivo by Lon and FtsH. J. Bacteriol. 180:1573-1577.
14. Lu, F., and G. Churchward. 1995. Tn916 target DNA sequences bind the C-terminal domain of integrase protein with different affinities that correlate with transposon insertion frequency. J. Bacteriol. 177:1938-1946.
15. Manganelli, R., S. Ricci, and G. Pozzi. 1996. Conjugative transposon Tn916: evidence for excision with formation of 5′-protruding termini. J. Bacteriol. 178:5813-5816.
16. Manganelli, R., L. Romano, S. Ricci, M. Zazzi, and G. Pozzi. 1995. Dosage of Tn916 circular intermediates in Enterococcus faecalis. J. Bacteriol. 34:48-57.
17. Marra, D., and J. R. Scott. 1999. Regulation of excision of the conjugative transposon Tn916. Mol. Microbiol. 31:609-622.
18. Perez-Casal, J., M. G. Caparon, and J. R. Scott. 1991. Mry, a trans-acting positive regulator of the M protein gene of Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems. J. Bacteriol. 173:2617-2624.
19. Poyart-Salmeron, C., P. Trieu-Cuot, C. Carlier, and P. Courvalin. 1989. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J. 8:2425-2433.
20. Rudy, C. K., J. R. Scott, and G. Churchward. 1997. DNA binding by the Xis protein of the conjugative transposon Tn916. J. Bacteriol. 179:2567-2572.
21. Scott, J. R., F. Bringel, D. Marra, G. Van Alstine, and C. K. Rudy. 1994. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol. Microbiol. 11:1099-1108.
22. Scott, J. R., and G. G. Churchward. 1995. Conjugative transposition. Annu. Rev. Microbiol. 49:367-397.
23. Scott, J. R., P. A. Kirchman, and M. G. Caparon. 1988. An intermediate in the transposition of the conjugative transposon Tn916. Proc. Natl. Acad. Sci. USA 85:4809-4813.
24. Storrs, M. J., C. Poyart-Salmeron, P. Trieu-Cuot, and P. Courvalin. 1991. Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase. J. Bacteriol. 173:4347-4352.
25. Taylor, K. L., and G. G. Churchward. 1997. Specific DNA cleavage mediated by the integrase of conjugative transposon Tn916. J. Bacteriol. 179:1117-1125.