Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense

Molecular and Biochemical Parasitology

Volumn 118, Issue 1, 2001, Pages 61-73

  Caffrey, Conor R ^a   Hansell, Elizabeth ^a   Lucas, Kimberley D ^a   Brinen, Linda S ^a   Alvarez Hernandez, Alejandro ^b   Cheng, Jiamning ^b   Gwaltney II, Stephen L ^b   Roush, William R ^b   Stierhof, York Dieter ^c   Bogyo, Matthew ^d   Steverding, Dietmar ^e   McKerrow, James H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c MAX PLANCK INST FÜR BIOLOGIE   (Germany)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Cathepsin; Recombinant expression; Rhodesain; Sleeping sickness; Trypanosome

Indexed keywords

ALANINE; ARGININE; BRUCIPAIN; CATHEPSIN; CRUZIPAIN; CYSTEINE PROTEINASE; CYSTEINE PROTEINASE INHIBITOR; DIPEPTIDE DERIVATIVE; EPOXIDE; GLUTAMIC ACID; RHODESAIN; SULFONE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME GLYCOSYLATION; ENZYME INHIBITION; ENZYME LOCALIZATION; ENZYME RELEASE; ENZYME STABILITY; LIFE CYCLE; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN MOTIF; TRYPANOSOMA BRUCEI; TRYPANOSOMA RHODESIENSE;

MAMMALIA; PICHIA PASTORIS; TRYPANOSOMA; TRYPANOSOMA BRUCEI RHODESIENSE;

EID: 0034767886     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00368-1     Document Type: Article

References (64)

1. Current public health status of trypanosomiasis and leishmaniasis
2. Measuring the costs of African animal trypanosomosis, the potential benefits of control and returns to research
3. Chemotherapy of human leishmaniasis and trypanosomiasis
4. Innate lack of susceptibility of Ugandan Trypanosoma brucei rhodesiense to DL-alpha-difluoromethylornithine (DFMO)
5. Antimalarial effects in mice of orally administered peptidyl cysteine protease inhibitors
6. Cysteine protease inhibitors cure an experimental Trypanosoma cruzi infection
7. Cysteine protease inhibitors as chemotherapy: Lessons from a parasite target
8. Cysteine protease inhibitors as chemotherapy for parasitic infections
9. Lysosomal and non-lysosomal peptidyl hydrolases of the bloodstream forms of Trypanosoma brucei brucei
10. Trypanosomiasis and trypanotolerance in cattle: A role for congopain
11. Proteases from Trypanosoma brucei brucei. Purification, characterisation and interactions with host regulatory molecules
12. Cysteine proteinase inhibitors kill cultured bloodstream forms of Trypanosoma brucei brucei
13. 2
14. Cysteine proteinases of trypanosome parasites: Novel targets for chemotherapy
15. A cysteine proteinase cDNA from Trypanosoma brucei predicts an enzyme with an unusual C-terminal extension
16. The C-terminal extension of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi
17. Leishmania major: Comparison of the cathepsin L- and B-like cysteine protease genes with those of other trypanosomatids
18. Activity and deletion analysis of recombinant human cathepsin L expressed in Escherichia coli
19. The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi
20. Cloning and sequencing of the cysteine protease cDNA from Trypanosoma brucei rhodesiense
21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
22. Antigenic variation in syringe passed populations of Trypanosoma (Trypanozoon) brucei I. Rationalization of the experimental approach
23. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose
24. Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium
25. Activity and structure relationship of acridine derivatives against African trypanosomes
26. Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers
27. Substrate binding and sequence preference of the proteasome revealed by active-site-directed affinity probes
28. Selective targeting of lysosomal cysteine proteases with radiolabeled electrophilic substrate analogs
29. Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy
30. A new method of preparing gold probes for multiple-labeling cytochemistry
31. Trypanosoma brucei rhodesiense: Membrane glycoproteins localized primarily in endosomes and lysosomes of bloodstream forms
32. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters
33. L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L
34. Theoretical and practical aspects of proteinase inhibition kinetics
35. A target within the target: Probing cruzain's P1′ site to define structural determinants for the Chagas' disease protease
36. Comparative protein modelling by satisfaction of spatial restraints
37. Schistosoma mansoni: Proteinase activity of "hemoglobinase" from the digestive tract of adult worms
38. Human cathepsin F. Molecular cloning, functional expression, tissue localization, and enzymatic characterization
39. Human cathepsin V. Functional expression, tissue distribution, electrostatic surface potential, enzymatic characterization, and chromosomal localization
40. Expression and deletion analysis of the Trypanosoma brucei rhodesiense cysteine protease in Escherichia coli
41. Primary structure and partial characterization of a life-cycle-regulated cysteine protease from Trypanosoma (Nannomonas) congolense
42. Genes for cysteine proteinases from Trypanosoma rangeli
43. Human cathepsins F and W: A new subgroup of cathepsins
44. Two distinct gene subfamilies within the family of cysteine protease genes
45. Proregion structure of members of the papain superfamily. Mode of inhibition of enzymatic activity
46. A developmentally regulated cysteine proteinase gene of Leishmania mexicana
47. The multiple cpb cysteine proteinase genes of Leishmania mexicana encode isoenzymes that differ in their stage regulation and substrate preferences
48. Molecular cloning, characterization and overexpression of two distinct cysteine protease cDNAs from Leishmania donovani chagasi
49. Identification of a developmentally regulated cysteine protease of Trypanosoma brucei
50. Endopeptidase variations among different life-cycle stages of African trypanosomes
51. Characterisation of a cysteine protease from bloodstream forms of Trypanosoma congolense
52. Human liver cathepsin L.
53. Trypanosoma brucei: Properties of extracellularly released proteases
54. Trypanosomatid cysteine protease activity may be enhanced by a kininogen-like moiety from host serum
55. On the size of the active site in proteases. I. Papain
56. Structural determinants of specificity in the cysteine protease cruzain
57. Vinyl sulfonate esters and vinyl sulfonamides: Potent, irreversible inhibitors of cysteine proteases
58. Isolation and characterization of E-64, a new thiol protease inhibitor
59. A new synthesis of peptidyl epoxysuccinates for probing cysteine protease-inhibitor P-3/S-3 binding interactions
60. Design, synthesis and evaluation of D-homophenylalanyl epoxysuccinate inhibitors of the trypanosomal cysteine protease cruzain
61. Refined x-ray structure of papain E-64-c complex at 2.1-Å resolution