Poly(A)-binding proteins regulate both mRNA deadenylation and decapping in yeast cytoplasmic extracts

RNA

Volumn 7, Issue 10, 2001, Pages 1416-1424

  Wilusz, Carol J ^a   Gao, Min ^b   Jones, Charles L ^a   Wilusz, Jeffrey ^b   Peltz, Stuart W ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

elF4E; mRNA decay; Poly(A) tail; Translation

Indexed keywords

BINDING PROTEIN; MESSENGER RNA; POLYADENYLIC ACID;

ADENYLATION; ARTICLE; CYTOPLASM; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; REGULATORY MECHANISM; RNA ANALYSIS; RNA DEGRADATION; RNA TRANSLATION; SACCHAROMYCES CEREVISIAE; YEAST;

ADENINE; BASE SEQUENCE; CYTOPLASM; DNA PRIMERS; POLY(A)-BINDING PROTEINS; RNA CAPS; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0034761729     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Altered mRNA cap recognition activity of initiation factor 4E in the yeast cell cycle division mutant cdc33
2. The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5
3. An essential component of the decapping enzyme required for normal rates of mRNA turnover
4. The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p
5. Messenger RNA turnover in cell-free extracts
6. Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation
7. PAN3 encodes a subunit of the Pablp-dependent poly(A) nuclease in Saccharomyces cerevisiae
8. Inhibition of mRNA turnover in yeast by an xrnl mutation enhances the requirement for elF4E binding to elF4G and for proper capping of transcripts by Ceg1p
9. Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast
10. AU-rich elements: Characterization and importance in m RNA degradation
11. A turnover pathway for both stable and unstable mRNAs in yeast: Evidence for a requirement for deadenylation
12. Cap-dependent deadenylation of mRNA
13. The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif
14. ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system
15. An in vitro system using HeLa cytoplasmic extracts that reproduces regulated mRNA stability
16. Interaction between a poly (A)-specific ribonuclease and the 5′ cap influences mRNA deadenylation rates in vitro
17. A novel mRNA decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements
18. The 3′ to 5′ degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SK12 DEVH box protein and 3′ to 5′ exonucleases of the exosome complex
19. RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G
20. The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes
21. Isolation and characterization of Dcpl p, the yeast mRNA decapping enzyme
22. Yeast mRNA splicing in vitro
23. The yeast RNA gene products are essential for mRNA splicing in vitro
24. The yeast poly(A)-binding protein Pablp stimulates in vitro poly(A)-dependent and cap-dependent translation by distinct mechanisms
25. Measurement of mRNA decay rates in Saccharomyces cerevisiae
26. A mutation in the tRNA nucleotidyltransferase gene promotes stabilization of mRNAs in Saccharomyces cerevisiae
27. A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: Domain structure and expression
28. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation
29. Mutations in translation initiation factors lead to increased rates of deadenylation and decapping of mRNAs in Saccharomyces cerevisiae
30. mRNA decapping in yeast requires dissociation of the cap binding protein, eukaryotic translation initiation factor 4E
31. Yeast Sm-like proteins function in mRNA decapping and decay
32. The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae
33. The eukaryotic mRNA decapping protein dcpl interacts physically and functionally with the elF4F translation initiation complex
34. An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro
35. Circularization of mRNA by eukaryotic translation initiation factors
36. The cap-to-tail guide to mRNA turnover
37. Mutations in VPS16 and MRT1 stabilize mRNAs by activating an inhibitor of the decapping enzyme
38. Monitoring mRNA decapping activity