Detection of caspase-9 activation in the cell death of the Bcl-x- deficient mouse embryo nervous system by cleavage sites-directed antisera

Developmental Brain Research

Volumn 122, Issue 2, 2000, Pages 135-147

  Fujita, Eriko ^a   Urase, Koko ^a   Egashira, Jun ^a   Miho, Yasuko ^a   Isahara, Kyoko ^b   Uchiyama, Yasuo ^b   Isoai, Atushi ^c   Kumagai, Hiroshi ^c   Kuida, Keisuke ^d   Motoyama, Noboru ^e   Momoi, Takashi ^a  

^a NATIONAL INSTITUTE OF NEUROSCIENCE   (Japan)

^b OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

^c ASAHI GLASS CO LTD   (Japan)

^d VERTEX PHARMACEUTICALS   (United States)

^e NATIONAL CENTER FOR GERIATRICS AND GERONTOLOGY   (Japan)

Author keywords

Apoptosis; Bcl xL; Caspase 9; Ced 3; Ced 9; Cytochrome c; Neuronal development

Indexed keywords

ANTISERUM; CASPASE 3; CASPASE 9; CYTOCHROME C OXIDASE; PROTEIN BCL 2; PROTEIN BCL X;

ANIMAL CELL; APOPTOSIS; ARTICLE; BRAIN REGION; CELL REGENERATION; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVATION; ENZYME DEFECT; FIBROBLAST; MOUSE; NERVE CELL NECROSIS; NERVOUS SYSTEM DEVELOPMENT; NEUROPROTECTION; NONHUMAN; PATHOPHYSIOLOGY; PRIORITY JOURNAL;

ANIMALS; BCL-X PROTEIN; CASPASE 9; CASPASES; CATTLE; CELL DEATH; CELLS, CULTURED; COS CELLS; CYTOCHROME C GROUP; EMBRYO; ENZYME ACTIVATION; EPITHELIAL CELLS; FETAL BLOOD; FIBROBLAST GROWTH FACTOR 2; FIBROBLASTS; GANGLIA, SPINAL; IMMUNE SERA; IN SITU NICK-END LABELING; MICE; MICE, KNOCKOUT; MICE, MUTANT STRAINS; NERVOUS SYSTEM; PEPTIDE FRAGMENTS; PROTO-ONCOGENE PROTEINS C-BCL-2; RHOMBENCEPHALON;

ANIMALIA; MAMMALIA;

EID: 0034734269     PISSN: 01653806     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-3806(00)00066-3     Document Type: Article

References (31)

1. Adams J.M., Cory S. The Bcl-2 protein family: arbiters of cell survival. Science. 281:1998;1322-1326.
2. Bossy-Wetzel E., Newmeyer D.D., Green D.R. Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization. EMBO J. 17:1998;37-49.
3. Devereaux Q.L., Takahashi R., Salvesen G.S., Reed J.C. X-linked IAP is a direct inhibitor of cell-death proteases. Nature. 388:1997;300-304.
4. Duan H., Orth K., Chinnaiyan A.M., Poirier G.G., Froelich C.J., He W.W., Dixit V.M. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271:1996;16720-16724.
5. Fujita E., Kouroku Y., Miho Y., Tsukahara T., Ishiura S., Momoi T. Wortmannin enhances activation of CPP32 (Caspase-3) induced by TNF or anti-Fas. Cell Death Differ. 5:1998;289-297.
6. Fujita E., Jinbo A., Matuzaki H., Konishi H., Kikkawa U., Momoi T. Akt motif found in human caspase-9 is absent in the mouse caspase-9. Biochem. Biophys. Res. Commun. 264:1999;550-555.
7. Gavrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:1992;493-501.
8. Graham F.L., van der Eb A.J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 52:1973;456-467.
9. Hakem R., Hakem A., Duncan G.S., Henderson J.T., Woo M., Soengas M.S., Wlia A., Pompa J.L., Kagi D., Khoo W., Potter J., Yoshida R., Kaufman S.A., Lowe S.W., Penninger J.M., Mak T.W. Differential requirement for caspase-9 in apoptostic pathway in vivo. Cell. 94:1998;339-352.
10. Hengartner M.O., Ellis R.E., Horvitz H.R. C. elegans cell survival gene ced-9 protects cells form programmed cell death. Nature. 356:1992;494-499.
11. Hu Y., Benedict M.A., Wu D., Inohara N., Nunez G. Bcl-xL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc. Natl. Acad. Sci. USA. 95:1998;4386-4391.
12. Keane R.W., Srinivasan A., Foster L.M., Testa M.P., Ord T., Nonner D., Wang H.G., Reed J.C., Bredesen D.E., Kayalar C. Activation of CPP32 during apoptosis of neurons and astrocytes. J. Neurosci. Res. 48:1997;168-180.
13. Kitani H., Shiurba R., Sakakura T., Tomooka Y. Isolation and characterization of mouse neural precursor cells in primary culture. In Vitro Cell Dev. Biol. 27A:1991;615-624.
14. Kouroku Y., Urase K., Fujita E., Isahara K., Ohsawa Y., Uchiyama Y., Momoi M.Y., Momoi T. Detection activated caspase-3 by a cleavage site-directed antiserum during naturally occurring DRG neurons apoptosis. Biochem. Biophys. Res. Commum. 247:1998;780-784.
15. Kuida K., Zheng T.S., Na S., Kuan C., Yang D., Karasuyama H., Rakic P., Flavell R.A. Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature. 384:1996;368-372.
16. Kuida K., Hayda T.F., Kuan C., Gu Y., Yaya C., Karasuyama H., Su M.S., Rakic P., Flavell R.A. Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase-9. Cell. 94:1998;325-337.
17. Li P., Nijhawan D., Budihardjo I., Srinivasula S.M., Ahmad M., Alnemri E.S., Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 91:1997;479-489.
18. Liston P., Roy N., Tamai K., Lefebvre C., Baird S., Cherton-Horvat G., Farahani R., McLean M., Ikeda J.E., MacKenzie A., Korneluk R.G. Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes. Nature. 379:1996;349-353.
19. Motoyama N., Wang F., Roth K.A., Sawa H., Nakayama K.-I., Nakayama K., Negishi I., Senju S., Zhang Q., Fujii S., Loh D.Y. Massive cell death of immature hematopoietic cells and neurons in Bcl-x-deficient mice. Science. 267:1995;1506-1510.
20. Mukasa T., Urase K., Momoi M.Y., Kimura I., Momoi T. Specific expression of CPP32 in sensory neurons of mouse embryos and activation of CPP32 in the apoptosis induced by a withdrawal of NGF. Biochem. Biophys. Res. Commun. 231:1997;770-774.
21. Nicholson D.W., Ali A., Thornberry N.A., Vaillancourt J.P., Ding C.K., Gallant M., Gareau Y., Griffin P.R., Labelle M., Lazebnik Y.A., Munday N.A., Raju S.M., Smulson M.E., Yamin T.-T., Yu V.L., Miller D.K. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 376:1995;37-43.
22. Pan G., O'Rourke K., Dixit V.M. Caspase-9, Bcl-xL, and Apaf-1 form a ternary complex. J. Biol. Chem. 273:1998;5841-5845.
23. Sakamaki K., Tsukumo S., Yonehara S. Molecular cloning and characterization of mouse caspase-8. Eur. J. Biochem. 253:1998;399-405.
24. Slee E.A., Harte M.T., Kluck R.M., Wolf B.B., Casiano C.A., Newmeyer D.D., Wang H.G., Reed J.C., Nicholson D.W., Alnemri E.S., Green D.R., Martin S.J. Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J. Cell Biol. 144:1999;281-292.
25. Srinivasula S.M., Fernandes-Alnemri T., Zangrilli J., Robertson N., Armstrong R.C., Wang L., Trapani J.A., Tomaselli K.J., Litwack G., Alnemri E.S. Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2 alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 271:1996;27099-27106.
26. Thornberry N.A., Lazebnik Y. Caspases: Enemies within. Science. 281:1998;1312-1316.
27. Urase K., Fujita E., Miho Y., Kouroku Y., Mukasa T., Yagi Y., Momoi M.Y., Momoi T. Detection of activated Caspase-3 (CPP32) in the vertebrate nervous system during development by a cleavage site-directed antiserum. Brain Res. Dev. Brain Res. 111:1998;77-87.
28. Urase K., Momoi T., Fujita E., Isahara K., Uchiyama Y., Tokunaga A., Nakayama K., Motoyama N. Bcl-xL is a negative regulator of caspase-3 activation in immature neurons during development. Brain Res. Dev. Brain Res. 116:1999;69-78.
29. Yuan J.Y., Horvitz H.R. The Caenorhabditis elegans genes ced-3 and ced-4 act cell autonomously to cause programmed cell death. Dev. Biol. 138:1990;33-41.
30. Yuan J.Y., Horvitz H.R. The Caenorhabditis elegans cell death gene ced-4 encodes a novel protein and is expressed during the period of extensive programmed cell death. Development. 116:1992;309-320.
31. Zou H., Henzel W.J., Liu X., Lutschg A., Wang X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell. 90:1997;405-413.