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At a concentration of 200 μg/ml, a 400-nm liposome contains ∼200 molecules of Sup35p. By comparison, the endogenous concentration of Sup35p is at least 20-fold less than that of ribosomes, which are present at ∼200,000 copies per yeast cell. See (35) for an estimate of ribosomes per yeast cell and (36, 37) for a quantification of Sup35p relative to ribosomes.
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We thank L. Gagne and M. Springer for assistance in liposome preparation and microscopy and F. E. Cohen, P. S. Kim, E. O'Shea, and members of the Lim and Weissman lab for helpful discussions. Supported by the NIH, the Searle Scholars Program, the David and Lucile Packard Foundation, and a postdoctoral fellowship from the European Molecular Biology Organization (H.E.S.).
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