Support for the prion hypothesis for inheritance of a phenotypic trait in yeast

Science

Volumn 273, Issue 5275, 1996, Pages 622-626

  Patino, Maria M ^a   Liu, Jia Jia ^a   Glover, John R ^a   Lindquist, Susan ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 104; PROTEIN SUP 35; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; MICROSCOPIC ANATOMY; PHENOTYPE; PRION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN ISOLATION; REVIEW; YEAST;

EID: 0029780647     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5275.622     Document Type: Review

References (37)

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33. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E. Glu; F, Phe; G. Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
34. 125I-conjugated protein A (ICN Pharmaceuticals), and then exposed to a Phosphorlmager screen (Molecular Dynamics).
35. +] aggregates have special properties, but we do not yet know if they form an ordered structure.
36. In preliminary experiments, GFP-marked prions do not disaggregate rapidly when Hsp104 is overexpressed (J.-J. Liu, unpublished data). Given their size, this is not surprising. However, because the prion assay relies on colony formation, and because Hsp104 is long-lived, it is possible that overexpression of Hsp104 simply prevents new prion conformers from joining the prion while preexisting prions are diluted by cell division
37. We thank Y. Chernoff, S. Liebman, I. Kerkatch, and M. F. Tuite for helpful discussions; M. F. Tuite for antibodies to Sup45; J. Warner for antibodies to ribosomal protein L3; M. D. Ter-Avananesyan and V. V. Kushnirov for plasmids and polyspecific Sup35 antisera; N. Patel for help with figures; and M. Singer, S. Rutherford, and S. K. DebBurman for comments on the manuscript. Supported by a grant from the National Institute of General Medical Sciences (NIH grant GM25874), and the Howard Hughes Medical Institute. J.-J.L. was supported by The Markey Program.