Creating a protein-based element of inheritance

Science

Volumn 287, Issue 5453, 2000, Pages 661-664

  Li, Liming ^a   Lindquist, Susan ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOCORTICOID RECEPTOR; HYBRID PROTEIN; PRION PROTEIN; TRANSLATION TERMINATION FACTOR;

ARTICLE; BIOENGINEERING; GENETIC TRANSFORMATION; INHERITANCE; NONHUMAN; PHENOTYPE; PRION DISEASE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RECOMBINANT DNA TECHNOLOGY; YEAST;

ANIMALS; FUNGAL PROTEINS; GENES, REPORTER; GUANIDINE; HEAT-SHOCK PROTEINS; PHENOTYPE; PRIONS; RATS; RECEPTORS, GLUCOCORTICOID; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC;

FUNGI; MYXOGASTRIA;

EID: 0034723391     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5453.661     Document Type: Article

References (30)

1. R. B. Wickner, Science 264, 566 (1994).
2. S. B. Prusiner, Proc. Natl. Acad. Sci. U.S.A. 95, 13363 (1998).
3. B. S. Cox, Heredity 24, 505 (1965).
4. F. Lacroute, J. Bacteriol. 106, 519 (1971).
5. I. Stansfield et al., EMBO J. 14, 4365 (1995).
6. M. M. Patino, J.-J. Liu, J. R. Glover, S. Lindquist, Science 273, 622 (1996).
7. J. R. Glover et al., Cell 89, 811 (1997).
8. C. Y. King et al., Proc. Natl. Acad. Sci. U.S.A. 94, 6618 (1997).
9. V. V. Kushnirov et al., Gene 66, 45 (1988).
10. M. D. Ter-Avanesyan et al., Mol. Microbiol. 7, 683 (1993).
11. Y. O. Chernoff, I. L. Derkach, S. G. Inge-Vechtomov, Curr. Genet. 24, 268 (1993).
12. M. D. Ter-Avanesyan, A. R. Dagkesamanskaya, V. V. Kushnirov, V. N. Smirnov, Genetics 137, 671 (1994).
13. I. L. Derkatch, Y. O. Chernoff, V. V. Kushnirov, S. G. Inge-Vechtomov, S. W. Liebman, Genetics 144, 1375 (1996).
14. J.-J. Liu and S. Lindquist, unpublished data.
15. C. Zhouravleva et al., EMBO J. 14, 4065 (1995).
16. M. Schena and K. R. Yamamoto, Science 241, 965 (1988).
17. 526 DNA fragment in a PCR reaction using pG1-NMGR as template. The PCR product was then digested with Bam HI, gel-purified, and subcloned into the Bam HI site of pG1.
18. L. Li and S. Lindquist, data not shown.
19. The ΔNMSUP35 74D-694 strain (gt12) is a gift from Y. O. Chernoff.
20. M. F. Tuite, C. R. Mundy, B. S. Cox, Genetics 98, 691 (1981).
21. Y. O. Chernoff, S. L. Lindquist, B. Ono, S. C. Inge-Vechtomov, S. W. Liebman, Science 268, 880 (1995).
22. The Hsp104 plasmid was eliminated by repetitively restreaking cells to nonselective media. Plasmid loss was confirmed by testing auxotrophy and by immunoblotting.
23. D. C. Masison, M. L. Maddelein, R. B. Wickner, Proc. Natl. Acad. Sci. U.S.A. 94, 12503 (1997).
24. M. M. Patino and S. Lindquist, unpublished data.
25. J.-J. Liu and S. Lindquist, Nature 400, 573 (1999).
26. D. J. Thiele, Mol. Cell. Biol. 8, 2745 (1988).
27. 4 to the concentrations as indicated in the Fig. 1 legend. Cells were harvested by centrifugation after overnight induction. Extracts were prepared by suspending the cell pellet (from a 3-ml culture) with 200 μl of lysis buffer containing 0.1 M potassium phosphate buffer (pH 7.8), 20% glycerol (v/v), 1 mM dithiothreitol, 2 μg/ml leupeptin, 2 mM benzamidine, and 1 mM phenylmethylsulfonyl fluoride. The cell suspension was mixed by vortexing 4 min at 4°C. After centrifugation at 1600g for 5 min, 5 μl of the lysate was used to measure β-galactosidase activity, using the Galacto-Light kit from TROPIX (Bedford, MA).
28. Use of a ΔNMSUP35 strain that already contains the reporter plasmid eliminates a low background of white colonies that arise from problems with two-plasmid cotransformation.
29. B. Gametchu and R. W. Harrison, Endocrinology 114, 274 (1984).
30. We thank K. Yamamoto for BuGR2 antibody; S. Liebman for the N expression plasmid, pEMBL-Δbal2, and the control plasmid, pEMBL-Yex4; M. Patino for the primers of NMGR; Lindquist lab members for comments on the manuscript; and J.-J. Liu for assistance in manuscript preparation. Supported by the Howard Hughes Medical Institute and NIH.