Wortmannin sensitizes human glioblastoma cell lines carrying mutant and wild type TP53 gene to radiation

Cancer Letters

Volumn 161, Issue 2, 2000, Pages 141-147

  Kubota, N ^a   Okada, S ^a   Inada, T ^a   Ohnishi, K ^b   Ohnishi, T ^b  

^a IBARAKI PREFECTURAL UNIVERSITY OF HEALTH SCIENCES   (Japan)

^b NARA MEDICAL UNIVERSITY   (Japan)

Author keywords

DNA PK; Radiosensitization; TP53; Wortmannin

Indexed keywords

PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN P53; WORTMANNIN;

ARTICLE; CONTROLLED STUDY; GLIOBLASTOMA; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IONIZING RADIATION; MUTANT; PRIORITY JOURNAL; RADIOSENSITIZATION; SIGNAL TRANSDUCTION; TUMOR SUPPRESSOR GENE;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANDROSTADIENES; BLOTTING, WESTERN; CELL SURVIVAL; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLINS; DOSE-RESPONSE RELATIONSHIP, RADIATION; GENES, P53; GLIOBLASTOMA; HUMANS; MUTATION; RADIATION TOLERANCE; RADIATION, IONIZING; RADIATION-SENSITIZING AGENTS; SIGNAL TRANSDUCTION; TIME FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P53; UP-REGULATION; X-RAYS;

EID: 0034695171     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3835(00)00614-5     Document Type: Article

References (34)

1. In vitro radiobiological parameters of human sarcoma cell lines
2. DNA double-strand break repair and V(D)J combination: Involvement of DNA-PK
3. Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line
4. Loss of catalytic subunit of the DNA-dependent protein kinase in DNA double-strand break repair mutant mammalian cells
5. The nuclear serine/threonine protein kinase DNA-PK
6. A binding of Ku protein to DNA: Measurement of affinity for ends and demonstrating of binding to niks
7. DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product
8. Wortmannin is a potent inhibitor of DNA double strand break but not single strand break repair in Chinese hamster ovary cells
9. The phosphatidylinositol 3-kinase inhibitor wortmannin sensitizes quiescent but not proliferating MG-63 human osteosarcoma cells to radiation
10. Wortmannin inhibits repair of DNA double-strand breaks in irradiated normal human cells
11. A single ataxia telangiectasia gene with a product similar to PI3-kinase
12. Induction of a particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells
13. Ataxia telangiectasia: A mutation with abnormal radiation sensitivity
14. Inhibition of phosphoinositide 3-kinase related kinases by the radiosensitizing agent wortmannin
15. A mammalian cell cycle checkpoint utilizing p53 and gadd45 is defective in ataxia telangiectasia
16. Enhanced phosphorylation of p53 by ATM in response to DNA damage
17. Activation of the ATM kinase by ionizing radiation and phophorylation of p53
18. Fixation and repair of radiation-induced potentially mutagenic damage sensitive to hypertonic treatment in human diploid fibroblasts
19. Bifunctional effects of a protein kinase inhibitor (H-7) on heat-induced p53-dependent WAF1 accumulation
20. Action of x-rays on mammalian cells
21. Human wild-type p53 adopts a unique conformational and phosphorylation state in vivo during growth arrest of glioblastoma cells
22. The phosphatidylinositol 3-kinase inhibitor wortmannin sensitizes murine fibroblasts and human tumor cells to radiation and blocks induction of p53 following DNA damage
23. Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction
24. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect
25. DNA-PK: At the cross roads of biochemistry and genetics
26. Wortmannin sensitizes mammalian cells to radiation by inhibiting the DNA-dependent protein kinase mediated rejoining of double-strand breaks
27. Cell cycle arrest and radiosensitivity of human tumor cell line: Dependence on wild-type p53 for radiosensitivity
28. Wild-type p53 is a cell cycle checkpoint determinant following irradiation
29. DNA-dependent protein kinase-independent activation of p53 in response to DNA damage
30. DNA-dependent protein kinase is not required for the p53-dependent response to DNA damage
31. A phosphatidylinositol 3-kinase inhibitor wortmannin induces radioresistant DNA synthesis and sensitizes cells to bleomycin and ionizing radiation
32. Effect of protein kinase inhibitors on the accumulation kinetics of p53 protein in normal human embryo cells following X-irradiation
33. WAF1, a potential mediator of p53 tumor suppression
34. Radiosensitization of human tumor cells by the phosphatidylinositol 3-kinase inhibitor wortmannin and LY294002 correlates with inhibition of DNA-dependent protein kinase and prolonged G2-M delay