Hammerhead ribozyme as a therapeutic agent for hyperlipidemia: Production of truncated apolipoprotein B and hypolipidemic effects in a dyslipidemia murine model

Human Gene Therapy

Volumn 11, Issue 17, 2000, Pages 2415-2430

  Enjoji, M ^a,b   Wang, F ^c   Nakamuta, M ^a,b   Chan, L ^a   Teng, B B ^a,c  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b KYUSHU UNIVERSITY   (Japan)

^c UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APOLIPOPROTEIN B; CHOLESTEROL; HAMMERHEAD RIBOZYME; MESSENGER RNA; RIBOZYME; TRIACYLGLYCEROL; UNCLASSIFIED DRUG; VIRUS VECTOR; APOLIPOPROTEIN B100; LIPOPROTEIN; RECOMBINANT PROTEIN;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; HYPERLIPIDEMIA; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; ANIMAL; BLOOD; DISEASE MODEL; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; HUMAN; LIVER; METABOLISM; METHODOLOGY; MOUSE MUTANT; TRANSGENIC MOUSE;

ADENOVIRIDAE; ANIMALIA; MURINAE;

ANIMALS; APOLIPOPROTEIN B-100; APOLIPOPROTEINS B; CHOLESTEROL; DISEASE MODELS, ANIMAL; GENE EXPRESSION REGULATION; HUMANS; HYPERLIPIDEMIAS; LIPOPROTEINS; LIVER; MICE; MICE, MUTANT STRAINS; MICE, TRANSGENIC; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RNA, CATALYTIC; TRIGLYCERIDES;

EID: 0034694055     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/104303400750038516     Document Type: Article

References (67)

1. Polymorphic DNA region adjacent to the 5' end of the human insulin gene
2. Anti-HIV therapeutic hammerhead ribozymes: Targeting strategies and optimization of intracellular function
3. The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization
4. The structure, function and application of the hammerhead ribozyme
5. Identification of the low density lipoprotein receptor-binding site in apolipoprotein B 100 and the modulation of its binding activity by the carboxyl terminus in familial defective apoB-100
6. Expression of human apolipoprotein B and assembly of lipoprotein (a) in transgenic mice
7. Ribozyme cleaves rex/tax mRNA and inhibits bovin leukemia virus expression
8. Apolipoprotein B-48 is the product of a mRNA with an organ-specific in-frame stop codon
9. New variation on the translocation of proteins during early biogenesis of apolipoprotein B
10. FPLC
11. Ribozyme mediated destruction of RNA in vivo
12. Regulation of hepatic secretion of apolipoprotein B-containing lipoproteins: Information obtained from cultured liver cells
13. Ribozyme-mediated attenuation of pancreatic B-cell glucokinase expression in transgenic mice results in impaired glucose-induced insulin secretion
14. Phenotypic analysis of mice expressing exclusively apolipoprotein B48 or apolipoprotein B 100
15. Neoplastic reversion accomplished by high efficiency adenoviral-mediated delivery of an anti-ras ribozyme
16. Chimeric DNA-RNA hammerhead ribozyme to proliferating cell nuclear antigen reduces stent-induced stenosis in a porcine coronary model
17. Enzymatic and antisense effects of a specific anti-Ki-ras ribozyme in vitro and in cell culture
18. Synthesis and secretion of apolipoprotein B from cultured liver cells
19. Hepatic gene transfer of the catalytic subunit of the apolipoprotein B mRNA editing enzyme results in a reduction of plasma LDL levels in normal and Watanabe heritable hyperlipidemic rabbits
20. Ribozyme-mediated inhibition of expression of leukocyte-type 12-lipoxygenase in porcine aortic vascular smooth muscle cells
21. Simple RNA enzymes with new and highly specific endoribonuclease activities
22. Numbering system for the hammerhead
23. Targeted modification of the apolipoprotein B gene results in hypobetalipoproteinemia and developmental abnormalities in mice
24. Gene transfer of cytidine deaminase apobec 1 lowers lipoprotein(a) in transgenic mice and induces apolipoprotein B editing in rabbits
25. Apolipoprotein B100 destined for lipoprotein assembly and intracellular degradation undergoes efficient translation across the endoplasmic reticulum membrane
26. Inhibition of vascular smooth muscle cell proliferation by ribozymes that cleave c-myb mRNA
27. HER-2/neu is rate-limiting for ovarian cancer growth: Conditional depletion of Her-2/neu by ribozyme targeting
28. Apolipoprotein B: Structural and metabolic heterogeneity
29. Towards an RNA-based therapy for Marfan syndrome
30. A gene-targeted mouse model for familial hypohetalipoproteinemia. Low levels of apolipoprotein B mRNA in association with a nonsense mutation in exon 26 of the apolipoprotein B gene
31. Dual mechanism for the low plasma levels of truncated apolipoprotein B proteins in familial hypobetalipoproteinemia
32. Hepatic expression of the catalytic subunit of the apolipoprotein B mRNA editing enzyme (apobec) ameliorates hypercholesterolemia in LDL receptor-deficient rabbits
33. 2-microglobulin mRNA levels in transgenic mice expressing a designed hammerhead ribozyme
34. Ribozyme rescue of photoreceptor cells in a transgenic rat model of autosomal dominant retinitis pigmentosa
35. Efficient and specific ribozyme-mediated reduction of bovine α-lactalbumin expression in double transgenic mice. Proc. Natl. Acad. Sci. U.S.A
36. Gene therapy targeting cord blood-derived CD38+ cells from HIV-exposed infants: Preclinical studies
37. Adenovirus-mediated expression of ribozymes in mice
38. Complete phenotypic characterization of apobec-1 knockout mice with a wild-type genetic background and a human apolipoprotein B mRNA editing by somatic gene transfer of apobec1
39. Suppression of the malignant phenotype of melanoma cells by anti-oncogene ribozymes
40. Use of bacterial expression cloning to localized the epitopes for a series of monoclonal antibodies against apolipoprotein B100
41. Hammerhead ribozymes targeted to the FBN mRNA can discriminate a single base mismatch between ribozyme and target
42. A novel form of tissue-specific RNA processing produces apolipoprotein B48 in intestine
43. A mouse model of human familial hypercholesterolemia: Markedly elevated low density lipoprotein cholesterol levels and severe atherosclerosis on a lowfat chow diet
44. The apolipoprotein B gene is constitutively expressed in HepG2 cells: Regulation of secretion by oleic acids, albumin, and insulin, and measurement of the mRNA half-life
45. Low expression of the apolipoprotein B mRNA editing transgene in mice reduces LDL but does not cause liver dysplasia or tumors
46. Intracellular cleavage of hepatitis C virus RNA and inhibition of viral protein translation by hammerhead ribozymes
47. Specific hammerhead ribozyme-mediated cleavage of mutant N-ras mRNA in vitro and ex vivo
48. Effective reversal of a transformed phenotype by retrovirus-mediated transfer of a ribozyme directed against mutant N-ras
49. Association of coronary atherosclerosis with hyperapobetalipoproteinemia (increased protein but normal cholesterol levels in human plasma low density lipoprotein)
50. Molecular bases of low production rates of apolipoprotein B-100 and truncated apoB-82 in a mutant HepG2 cell line generated by targeted modification of the apolipoprotein B gene
51. Lipoproteins and atherogenesis
52. Expression of a chimeric ribozyme gene results in endonucleolytic cleavage of target mRNA and a concomitant reduction of gene expression in vivo
53. The use of ribozymes to inhibit HIV replication
54. Small catalytic RNAs
55. Metabolic basis of hyperapobetalipoproteinemia: Turnover of apoB in LDL and its precursors and subfractions compared with normal and familial hypercholesterolemia
56. ApoB mRNA editing is developmentally regulated in pig small intestine: Nucleotide comparison of apoB mRNA editing regions in five species
57. Molecular cloning of an apoB mRNA editing protein
58. Adenovirus-mediated gene transfer of rat apoB mRNA editing protein in mice virtually eliminates apoB-100 and normal LDL production
59. Effective lowering of plasma, LDL, and esterified cholesterol in LDL receptor-knock mice by adenovirus-mediated gene delivery of apoB mRNA editing enzyme (apobec1)
60. A small catalytic oligoribonucleotide
61. Targeted reduction of nucleoside triphosphate hydrolase by antisense RNA inhibits Toxoplasma gondii proliferation
62. Hammerhead ribozyme cleavage of apolipoprotein B mRNA generates a truncated protein
63. A ribozyme-mediated, gene 'knockdown' strategy for the identification of gene function in zebrafish
64. Apolipoprotein B mRNA editing protein induces hepatocellular carcinoma and dysplasia in transgenic animals
65. Synthesis and secretion of hepatic apolipoprotein B-containing lipoproteins
66. Intracellular degradation of newly synthesized apolipoprotein B
67. Generating loss-of-function phenotypes of the fushi tarazu gene with a targeted ribozyme in Drosophila