Detection of rodent liver carcinogen genotoxicity by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow)

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 391, Issue 3, 1997, Pages 201-214

  Sasaki, Yu F ^a   Izumiyama, Fusako ^a   Nishidate, Emi ^a   Matsusaka, Naonori ^b   Tsuda, Shuji ^b  

^a HACHINOHE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

^b IWATE UNIVERSITY   (Japan)

Author keywords

Alkaline single cell gel electrophoresis (SCG) assay; Comet assay; DNA lesion; Liver; Liver carcinogen; Mouse; Multiple organ

Indexed keywords

2 IMIDAZOLIDINETHIONE; 2,4 DIAMINOTOLUENE; 4 AMINOAZOBENZENE; AURAMINE; CARCINOGEN; DNA; LINDANE; PHENOBARBITAL; STYRENE OXIDE;

ALKALINITY; ANIMAL EXPERIMENT; ARTICLE; BONE MARROW; BONE MARROW CELL; CONTROLLED STUDY; DNA DAMAGE; GEL ELECTROPHORESIS; GENOTOXICITY; HUMAN TISSUE; INTRAPERITONEAL DRUG ADMINISTRATION; KIDNEY; LIVER; LIVER CARCINOGENESIS; LUNG; MALE; MICRONUCLEUS TEST; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN; TECHNIQUE; TISSUE HOMOGENATE;

ANIMALS; BENZOPHENONEIDUM; BONE MARROW; CARCINOGENS; CHLOROBENZENES; ELECTROPHORESIS; EPOXY COMPOUNDS; ETHYLENETHIOUREA; KIDNEY; LINDANE; LIVER; LUNG; MALE; MICE; MICE, INBRED STRAINS; MICRONUCLEUS TESTS; MUTAGENICITY TESTS; ORGAN SPECIFICITY; P-AMINOAZOBENZENE; PHENOBARBITAL; PHENYLENEDIAMINES; SPLEEN;

ANIMALIA; RODENTIA;

EID: 0030816769     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(97)00072-7     Document Type: Article

References (37)

1. N.P. Singh, M.T. McCoy, R.R. Tice, E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell. Res. 175 (1988) 184-191.
2. P. Schmezer, C. Eckert, U.M. Liegibel, Tissue-specific induction of mutations by streptozotocin in vivo, Mutation Res. 307 (1994) 495-499.
3. D.K. Monteith, J. Vanstone, Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the induction of DNA damage, Mutation Res. 345 (1995) 97-103.
4. N.P. Singh, H. Lai, A. Khan, Ethanol-induced single-strand DNA breaks in rat brain cells, Mutation Res. 345 (1995) 191-196.
5. M. Altamirano-Lozano, L. Alvarez-Barrera, F. Basurto-Alcanyara, M. Valverde, E. Rojas, Reprotoxic and genotoxic studies of vanadium pentaoxide in male mice, Teratog. Carcinog. Mutag. 16 (1996) 7-17.
6. D.W. Fairbairn, P.L. Olive, K.L. O'Neill, The comet assay: a comprehensive review, Mutation Res. 339 (1995) 37-59.
7. Y.F. Sasaki, Y.F., S. Tsuda, F. Izumiyama, E. Nishidate, Detection of chemically induced DNA lesions in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow) using the alkaline single cell gel electrophoresis (Comet) assay, Mutation Res. 388 (1997) 33-44.
8. T. Morita, N. Asano, T. Awogi, Y.F. Sasaki, S. Sato, H. Shimada, S. Sutou, T. Suzuki, A. Wakata, T. Sofuni, M. Hayashi, Evaluation of the rodent micronucleus assay to screen IARC carcinogens (group 1, 2A, and 2B), The summary report of the 6th collaborative study by CSGMT/JEMS.MMS, Mutation Res. 389 (1997) 2-122.
9. Y.F. Sasaki, S. Tsuda, E. Nishidate, F. Izumiyama, Simple detection of chemical mutagens by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow), Mutation Res. 391 (1997) 215-231.
10. A. Goldstein, Biostatistics. An Introductory Text, Macmillan, New York, 1964, pp. 49-51.
11. E. Zeiger, B. Anderson, S. Haworth, T. Lawlor, K. Mortelmans, Salmonella mutagenicity tests: V. Results from the testing of 311 chemicals, Environ. Mol. Mutagen. 21 (Suppl. 19) (1992) 2-141.
12. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 7, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, p. 390.
13. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 6, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 83-85.
14. M. Ishidate Jr., M.C. Harnois, T. Sofuni, A comparative analysis of data on the clastogenicity of 951 chemical substances tested in mammalian cell cultures, Mutation Res. 195 (1988) 151-213.
15. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 7, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 118-119.
16. J. Ashby, R.W. Tennant, Definitive relationships among chemical structure, carcinogenicity and mutagenicity for 301 chemicals tested by the US NTP, Mutation Res. 257 (1991) 229-306.
17. K.S. Loveday, B.E. Anderson, M.A. Resnick, E. Zeiger, Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro: II. Results with 20 chemicals, Environ. Mol. Mutagen. 16 (1990) 272-303.
18. W.J. Coppinger, S.A. Brennan, J.H. Carver, E.D. Thompson, Locus specificity of mutagenicity of 2,4-diaminotoluene in both L5178Y mouse lymphoma and AT3-2 Chinese hamster ovary cells, Mutation Res. 135 (1984) 115-123.
19. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Vol. 16, International Agency for Research on Cancer, Lyon, 1978, pp. 83-91.
20. E. Zeiger, J.K. Haseman, M.D. Shelby, B.H. Margolin, R.W. Tennant, Evaluation of four in vitro genetic toxicity tests for predicting rodent carcinogenicity: confirmation of earlier results with 41 additional chemicals, Environ. Mol. Mutagen. 16 (Suppl. 18) (1990) 1-14.
21. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 6, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 224-224.
22. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 7, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 192-193,
23. L.S. Gold, T.H. Slone, B.R. Stern, L. Bernstein, Comparison of target organs of carcinogenicity for mutagenic and non-mutagenic chemicals, Mutation Res. 286 (1993) 75-100.
24. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 6, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 304-307.
25. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 7, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 207-208.
26. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 6, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 455-458.
27. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Suppl. 7, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 313-316.
28. IARC, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Vol. 60, International Agency for Research on Cancer, Lyon, 1994, pp. 321-346.
29. H. Suzuki, C. Watanabe, S. Nakane, 1,2,3,4,5,6-Hexachlorocyclohexane does not induce micronucleus in mice, MMS Commun. 2 (1994) 41-43.
30. IARC, IARC monographs on the evaluation of carcinogenic risks to humans, Suppl. 6, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 333-335.
31. IARC, IARC monographs on the evaluation of carcinogenic risks to humans, Suppl. 7, Overall Evaluation of Carcinogenicity, An Updating of IARC Monographs from Vols. 1-42, International Agency for Research on Cancer, Lyon, 1987, pp. 220-222.
32. S. Gad, A. Smith, A. Cramp, F. Gavigan, M. Derelanko, Innovative designs and practicies for acute systemic toxicity studies, Drug Chem. Toxicol. 7 (1984) 423-434.
33. J.O. Macnamara, Drugs effective in the therapy of the epilepsies, in: Hardman, J.G. and Limbird, L.E. (Eds.), Goodman and Gilman's the Pharmacological Basis of Therapeutics, 9th edn., McGraw-Hill, New York, 1996, pp. 461-486.
34. G.B. Corcoran, L. Fix, D.P. Jones, M.T. Moslen, P. Nicotera, F.A. Oberhammer, R. Buttyan, Apoptosis: molecular control point in toxicity, Toxicol. Appl. Pharmacol. 128 (1994) 169-181.
35. Z. Gregus, C.D. Klaassen, Mechanims of toxicity, in: C.D. Klaassen (Ed.), Casarett and Doull's Toxicology, The Basic Science of Poisons, 5th edn., McGraw-Hill, New York, 1996, pp. 35-74.
36. R. Vijayalaxmi, R. Tice, G.H.S. Strauss, Assessment of radiation-induced DNA damage in human lymphocytes using single cell gel electrophoresis technique, Mutation Res. 271 (1992) 243-252.
37. M. Hayashi, S. Sutou, H. Shimada, S. Sato, Y.F. Sasaki, A. Wakata, Difference between intraperitoneal and oral gavage application in the micronucleus test, Mutation Res. 223 (1989) 329-344.