Hiv-1 reverse transcriptase-pseudoknot RNA aptamer interaction has a binding affinity in the low picomolar range coupled with high specificity

Journal of Biological Chemistry

Volumn 275, Issue 24, 2000, Pages 18271-18278

  Kensch, Oliver ^a   Connolly, Bernard A ^b   Steinhoff, Heinz Jürgen ^a   McGregor, Alistair ^b   Goody, Roger S ^a   Restle, Tobias ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

RNA DERIVATIVE; RNA DIRECTED DNA POLYMERASE; RNA DIRECTED DNA POLYMERASE INHIBITOR;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DISSOCIATION; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME INHIBITOR INTERACTION; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING;

CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; HIV-1 REVERSE TRANSCRIPTASE; HUMANS; KINETICS; LIGANDS; MICROCHEMISTRY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PROTEIN CONFORMATION; RNA;

HIV 1; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2; RNA VIRUSES;

EID: 0034674431     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M001309200     Document Type: Article

References (52)

1. Jäger, J., and Pata, J. D. (1999) Curr. Opin. Struct. Biol. 9, 21-28
2. De-Clercq, E. (1999) I/Farmaco 54, 26-45
3. Vandamme, A. M., Van Laethem, K., and De-Clercq, E. (1999) Drugs 57, 337-361
4. Gold, L. (1995) J. Biol. Chem. 270, 13581-13584
5. Tuerk, C., and Gold, L. (1990) Science 249, 505-510
6. Tuerk, C., Macdougal, S., and Gold, L. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 6988-6992
7. Green, L., Waugh, S., Binkley, J. P., Hostomska, Z., Hostomsky, Z., and Tuerk, C. (1995) J. Mol. Biol. 247, 60-68
8. Burke, D. H., Scates, L., Andrews, K., and Gold, L. (1996) J. Mol. Biol. 264, 650-666
9. Rietveld, K., Van Poelgeest, R., Pleij, C. W., Van Boom, J. H., and Bosch, L. (1982) Nucleic Acids Res. 10, 1929-1946
10. Studnicka, G. M., Rahn, G. M., Cummings, I. W., and Salser, W. A. (1978) Nucleic Acids Res. 5, 3365-3387
11. Ferre-D'Amare, A. R., Zhou, K. H., and Doudna, J. A. (1998) Nature 395, 567-574
12. Deiman, B. A. L. M., and Pleij, C. W. A. (1997) Semin. Virol. 8, 166-175
13. Puglisi, J. D., Wyatt, J. R., and Tinoco, I. J. (1990) J. Mol Biol. 214, 437-453
14. Shen, L. X., and Tinoco, I. (1995) J. Mol. Biol. 247, 963-978
15. Du, Z. H., Giedroc, D. P., and Hoffman, D. W. (1996) Biochemistry 35, 4187-4198
16. Du, Z. H., Holland, J. A., Hansen, M. R., Giedroc, D. P., and Hoffman, D. W. (1997) J. Mol. Biol. 270, 464-470
17. Kang, H. S., and Tinoco, I. (1997) Nucleic Acids Res. 25, 1943-1949
18. Kolk, M. H., Vandergraaf, M., Wijmenga, S. S., Pleij, C. W. A., Heus, H. A., and Hilbers, C. W. (1998) Science 280, 434-438
19. Su, L., Chen, L. Q., Egli, M., Berger, J. M., and Rich, A. (1999) Nat. Struct. Biol. 6, 285-292
20. Jäger, J., Restle, T., and Steitz, T. A. (1998) EMBO J. 17, 4535-4542
21. Rodgers, D. W., Gamblin, S. J., Harris, B. A., Ray, S., Culp, J. S., Hellmig, B., Woolf, D. J., Debouck, C., and Harrison, S. C. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1222-1226
22. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., and Pease, L. R. (1989) Gene 77, 51-59
23. Müller, B., Restle, T., Weiss, S., Gautel, M., Sczakiel, G., and Goody, R. S. (1989) J. Biol. Chem. 264, 13975-13978
24. Le Grice, S. F., Naas, T., Wohlgensinger, B., and Schatz, O. (1991) EMBO J. 10, 3905-3911
25. Certa, U., Bannwarth, W., Stüber, D., Gentz, R., Lanzer, M., Legrice, S. F. J., Gulliot, F., Wendler, I., Hunsmann, G., Bujard, H., and Mous, J. (1986) EMBO J. 5, 3051-3056
26. Müller, B., Restle, T., Kühnel, H., and Goody, R. S. (1991) J. Biol. Chem. 266, 14709-14713
27. Souquet, M., Restle, T., Krebs, R., Legrice, S. F. J., Goody, R. S., and Wöhrl, B. M. (1998) Biochemistry 37, 12144-12152
28. Wöhrl, B. M., Krebs, R., Thrall, S. H., Le Grice, S. F. J., Scheidig, A. J., and Goody, R. S. (1997) J. Biol. Chem. 272, 17581-17587
29. Thrall, S. H., Krebs, R., Wöhrl, B. W., Cellai, L., Goody, R. S., and Restle, T. (1998) Biochemistry 37, 13349-13358
30. Krebs, R., Immendorfer, U., Thrall, S. H., Wöhrl, B. M., and Goody, R. S. (1997) Biochemistry 36, 10292-10300
31. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1994) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
32. McGregor, A., Murray, J. B., Adams, C. J., Stockley, P. C., and Connolly, B. A. (1999) J. Biol. Chem. 274, 2255-2262
33. Restle, T., Müller, B., and Goody, R. S. (1990) J. Biol. Chem. 265, 8986-8988
34. Jacques, P. S., Wöhrl, B. M., Ottmann, M., Darlix, J. L., and Le Grice, S. F. J. (1994) J. Biol. Chem. 269, 26472-26478
35. Divita, G., Müller, B., Immendörfer, U., Gautel, M., Rittinger, K., Restle, T., and Goody, R. S. (1993) Biochemistry 32, 7966-7971
36. Müller, B., Restle, T., Reinstein, J., and Goody, R. S. (1991) Biochemistry 30, 3709-3715
37. Steinhoff, H. J., Radzwill, N., Thevis, W., Lenz, V., Brandenburg, D., Antson, A., Dodson, G., and Wollmer, A. (1997) Biophys. J. 73, 3287-3298
38. Steinhoff, H. J., Dombrowsky, O., Karim, C., and Schneiderhahn, C. (1991) Eur. Biophys. J. 20, 293-303
39. Rabenstein, M. D., and Shin, Y. K. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 8239-8243
40. Thorgeirsson, T. E., Xiao, W. Z., Brown, L. S., Needleman, R., Lanyi, J. K., and Shin, Y. K. (1997) J. Mol. Biol. 273, 951-957
41. Mchaourab, H. S., Oh, K. J., Fang, C. J., and Hubbell, W. L. (1997) Biochemistry 36, 307-316
42. Closs, G. L., Forbes, M. D. E., and Piotrowiak, P. (1992). J. Am. Chem. Soc. 114, 3285-3294
43. Fiori, W. H., and Millhauser, G. L. (1995) Biopolymers 37, 243-250
44. Rittinger, K., Divita, G., and Goody, R. S. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 8046-8049
45. Wöhrl, B. M., Krebs, R., Goody, R. S., and Restle, T. (1999) J. Mol. Biol. 292, 333-344
46. Hubbell, W. L., Gross, A., Langen, R., and Lietzow, M. A. (1998) Curr. Opin. Struct. Biol. 8, 649-656
47. Tiebel, B., Radzwill, N., Aung-Hilbrich, L. M., Helbl, V., Steinhoff, H. J., and Hillen, W. (1999) J. Mol. Biol. 290, 229-240
48. Pfeiffer, M., Rink, T., Gerwert, K., Oesterhelt, D., and Steinhoff, H. J. (1999) J. Mol. Biol. 287, 163-171
49. Jacobo-Molina, A., Ding, J., Nanni, R. G., Clark, A. D., Lu, X., Tantillo, C., Williams, R. L., Kamer, G., Ferris, A. L., Clark, P., Hizi, A., Hughes, S. H., and Arnold, E. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 6320-6324
50. Huang, H., Chopra, R., Verdine, G. L., and Harrison, S. C. (1998) Science 282, 1669-1675
51. Hsiou, Y., Ding, J., Das, K., Clark, A. D., Jr., Hughes, S. H., and Arnold, E. (1996) Structure 4, 853-860
52. Patel, D. J., Suri, A. K., Jiang, F., Jiang, L., Kumar, P. F., and Nonin, S. (1997) J. Mol. Biol. 272, 645-664