Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin α-Sarcin

Proteins: Structure, Function and Genetics

Volumn 41, Issue 3, 2000, Pages 350-361

  De Antonio, Carmen ^a   Del Pozo, Alvaro Martinez ^a   Mancheno, Jose M ^a   Onaderra, Mercedes ^a   Lacadena, Javier ^a   Martinez Ruiz, Antonio ^a   Perez Canadillas, Jose M ^b   Bruix, Marta ^b   Gavilanes, Jose G ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b INSTITUTO DE ESTRUCTURA DE LA MATERIA   (Spain)

Author keywords

Aspergillus protein; Cytotoxin; Protein spectroscopy; Ribosome inactivating protein; Site directed mutagenesis

Indexed keywords

ALPHA SARCIN; RNA; TRYPTOPHAN;

ARTICLE; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS;

ANTHRACENES; CIRCULAR DICHROISM; CYTOTOXINS; ENDORIBONUCLEASES; FLUORESCENCE POLARIZATION; FUNGAL PROTEINS; HEAT; MODELS, MOLECULAR; MOLECULAR PROBES; MUTATION; MYCOTOXINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHATIDYLGLYCEROLS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN SYNTHESIS INHIBITORS; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; TRYPTOPHAN;

ASPERGILLUS; ASPERGILLUS GIGANTEUS;

EID: 0034669878     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0134(20001115)41:3<350::AID-PROT70>3.0.CO;2-V     Document Type: Article

References (48)

1. Ribosome inactivating proteins from plants: Present status and future prospects
2. Determination of the 28S ribosomal RNA identity element (G4319) for α-sarcin and the relationship of recognition to the selection of the catalytic site
3. Specific cleavage of ribosomal RNA caused by α-sarcin
4. Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818
5. Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed
6. Fungal ribotoxins: A family of naturally engineered targeted toxins?
7. Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein α-sarcin
8. A single amino acid substitution in ribonucleolytic toxin restrictocin abolishes its specific substrate recognition activity
9. Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin α-sarcin
10. 15N nuclear magnetic resonance assignment and secondary structure of the cytotoxic ribonuclease α-sarcin
11. Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease α-sarcin
12. Insights into specificity of cleavage and mechanism of cell entry from the crystal structure of the highly specific Aspergillus ribotoxin, restrictocin
13. Predictive study of the conformation of the cytotoxic protein α-sarcin: A structural model to explain α-sarcin-membrane interaction
14. Fusion of phospholipid vesicles produced by the anti-tumour protein α-sarcin
15. Study of the interaction between the antitumour protein α-sarcin and phospholipid vesicles
16. Translocation of α-sarcin across the lipid bilayer of asolectin vesicles
17. Kinetic study of the cytotoxic effect of α-sarcin, a ribosome inactivating protein from Aspergillus giganteus, on tumour cell lines: Protein biosynthesis inhibition and cell binding
18. Molecular interactions involved in the passage of the cytotoxic protein α-sarcin across membranes
19. Spectroscopic characterization of the alkylated α-sarcin cytotoxin: Analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction
20. Overproduction and purification of biologically active native fungal α-sarcin in Escherichia coli
21. Rapid and efficient site-specific mutagenesis without phenotypic selection
22. Use of T7 RNA polymerase to direct expression of cloned genes
23. Secretion cloning vectors in Escherichia coli
24. pEMBL: A new family of single stranded plasmids for sequencing DNA
25. α-Sarcin, a new antitumor agent. I. Isolation, purification, chemical composition and the identity of a new amino acid
26. Kinetic study of the aggregation and lipid-mixing produced by α-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: Stopped-flow light scattering and fluorescence energy transfer measurements
27. Membrane interaction of a β-structure forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein α-sarcin
28. Colorimetric assay methods for free and phosphorylated glyceric acids
29. Use of resonance energy transfer to monitor membrane fusion
30. Interaction of dipalmitoylphosphatidylcholine with calf thymus histone H1
31. How to measure and predict the molar absorption coefficient of a protein
32. Characterization of pKa values and titration shifts in the cytotoxic ribonuclease α-sarcin by NMR. Relationship between electrostatic interactions, structure and catalytic function
33. Thermal unfolding of the cytotoxin α-sarcin: Phospholipid binding induces destabilization of the protein structure
34. Effect of the antitumour protein α-sarcin on the thermotropic behaviour of acid phospholipid vesicles
35. Aromatic side-chain contributions to the far ultraviolet circular dichroism of peptides and proteins
36. Convex constraint analysis: A natural deconvolution of circular dichroism curves
37. The cation-π interaction
38. Quenching of tryptophan fluorescence by the active-site disulfide bridge in the DsbA protein from Escherichia coli
39. Tryptophan fluorescence study of conformational transitions of the oxidized and reduced form of thioredoxin
40. Fluorescence analysis of tryptophan-containing variants of the LamB signal sequence upon insertion into a lipid bilayer
41. Acrylamide quenching of the intrinsic fluorescence of tryptophan residues genetically engineered into the soluble colicin-E1 channel peptide. Structural characterization of the insertion-competent state
42. Fluorescence of membrane-bound tryptophan octyl ester: A model for studying intrinsic fluorescence of protein-membrane interactions
43. Structure-function studies of tryptophan mutants of equinatoxin II, a sea anemone pore-forming protein
44. Interaction of the lantibiotic nisin with membrane revealed by fluorescence quenching of an introduced tryptophan
45. Investigation on the interactions of peptides in the assembly of liposome and peptide by fluorescence
46. MOLMOL: A program for display and analysis of macromolecular structures